FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_20.3410000000569f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_20.3410000000569f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436921
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCG250.002104736236.00329616
AATCGAC250.002104736236.00329616
TTCGTAC352.8179138E-432.1421217
GTAATAC406.160801E-428.1243573
TTATACT406.160801E-428.1243574
TGTAATA406.160801E-428.1243572
CTACCGA450.001223866625.0051521
GGGGCGG602.464496E-422.49948519
CGGGTAC500.002262681722.49948317
TCTCCGA6250.021.23951345
TGTAAGT654.233092E-420.7687572
GCCTAAC550.003928001520.4587611
AATTCGT550.00393060720.45641915
CTAATCG550.003933213720.45407924
AATCGCC550.003933213720.45407926
CGCCATA550.003933213720.45407929
TAATCGC550.003933213720.45407925
AAGTGTA600.006505047418.749577
ACGGGTA851.20142955E-418.53110716
TAGGACA750.001108896817.9995884
Produced by FastQC (version 0.11.2)