FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_2.3410000000550c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_2.3410000000550c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278611
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGTA301.13773014E-437.4998520
GTATACA300.00513623530.0052621
GGTATCA1053.6379788E-1227.8620321
GTATCAA2350.023.940371
GTATTGG1450.023.2799471
TATACAT602.4610726E-422.499915
CCAATCG500.002260391422.49990815
ACAATGC754.5867204E-520.9999168
CCAATAC654.22725E-420.76914835
TCGTTGT550.003929262520.45446419
AGGGTGT550.003929262520.4544646
CATTATA550.003929262520.45446432
TTAGTAG550.003929262520.4544643
GCTCTGA807.526605E-519.6909561
ACGAATT706.965498E-419.28563721
TTCGTAC1302.7499482E-819.03838517
TTAGGTA600.0064985618.74992631
TTAACAG600.0064985618.7499264
ATTATAT600.0064985618.74992633
CAATCGT600.0064985618.74992616
Produced by FastQC (version 0.11.2)