FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_18.34100000005675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_18.34100000005675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422961
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGTA352.8187738E-432.14015619
GGTATCA3300.031.3869721
GTATCAA7350.025.4270691
TCTCCGA4050.020.55625745
TGGAGCG550.003934403420.4528265
CTAAGGT807.553458E-519.6858444
CGGGGGA807.553458E-519.68584417
CGTTTGT706.9774326E-419.28409415
TAGTATG706.9774326E-419.2840945
TCGTTTA600.006507009318.74842312
AACAGCG600.006507009318.7484237
TGTCCGC600.006507009318.74842310
ACGGGGG1851.6370905E-1118.24170916
TAAGGTG1002.0431462E-517.9984875
TATCAAC9900.017.9530372
ATCTCCG7000.017.67917645
CATCTCC14950.017.60929745
ACATCTC22050.017.3475345
ATCAACG10000.017.3235443
CAACGCA9900.017.2712765
Produced by FastQC (version 0.11.2)