FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_17.34100000005668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_17.34100000005668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282989
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA21940.7752951528151271No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA21600.7632805515408726No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC18120.6403075737926209No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCG17770.6279396018926531No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG16770.5926025393213163No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC15370.543130651721445No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG14130.49931269413298746No Hit
GTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCG12980.4586750721759503No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATACA12250.4328790164988745No Hit
GATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAG10930.3862340939047101No Hit
TATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGAG10350.36573859761333477No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACA9830.34736332507623974No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATA9290.32828131128771787No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATA8900.31449985688489657No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG8610.30425210873920894No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCG8130.28729031870496735No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATA8100.2862302068278272No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTAT7990.28234312994498023No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA7690.2717420111735792No Hit
TGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA7150.25265999738505734No Hit
TATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCCGA6480.22898416546226177No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCACTGTCTCTTATACACATCTCC6290.2222701235737078No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCC6280.22191675294799446No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTAT6190.21873641731657414No Hit
TGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC6190.21873641731657414No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCC6130.21661619356229395No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTATAC6070.21449596980801372No Hit
ATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCC6020.2127291166794469No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCTGTCTCTTATACACATCTCCGA5700.20142125665661917No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT5670.20036114477947906No Hit
ATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA5650.1996544035280523No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCC5580.19718080914805877No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATC5270.18622631975094436No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC5150.181985872242384No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCCTGTCTCTTATACACATCT5060.17880553661096368No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTAT5010.17703868348239685No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA5000.17668531285668349No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAACTGTCTCTTATA4950.17491845972811665No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCT4890.17279823597383645No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.16926452971670278No Hit
TTTTTGCGTTGATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATA4750.1678510472138493No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTA4580.16184374657672207No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACA4550.16078363469958196No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCT4460.15760329906816167No Hit
TTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTAT4450.15724992844244828No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.154069592811028No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATAC4350.1537162221853146No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCTGTCTCTTATACACATCTC4290.15159599843103444No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACA4210.1487690334253275No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.14382184466534034No Hit
ATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGA4040.14276173278820026No Hit
GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3970.1402881384082067No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTC3910.13816791465392647No Hit
TTTTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTA3760.132867355268226No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3750.1325139846425126No Hit
GGTATCAACGCAGAGTACGGGTGGTATCCTGTCTCTTATACACATCTCCGA3710.13110050213965913No Hit
TGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3700.13074713151394576No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAACTGTCTCTTAT3690.1303937608882324No Hit
GATACCACTGCCCGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCG3600.1272134252568121No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATC3570.126153313379672No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAACTGTCTCTTATA3560.12579994275395862No Hit
GTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTC3540.1250932015025319No Hit
TTTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTAT3480.1229729777482517No Hit
GATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA3470.12261960712253833No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCTGTCTCTTATACACATCTCCGA3390.1197926421168314No Hit
GGTATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTC3380.11943927149111802No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAACTGTCTCTT3350.11837915961397792No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCCTGTCTCTTATACACATCTCCG3270.11555219460827099No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAACTGTCTCTTA3260.11519882398255764No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCCTGTCTCTTATACACATCT3260.11519882398255764No Hit
GTTGATACCACTGCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC3220.11378534147970416No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACCTGTCTCTTATACACATCTC3220.11378534147970416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.11307860022827741No Hit
TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAACTGTCTCTTATACAC3130.11060500584828385No Hit
GATACCACTCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG3050.10777804084257693No Hit
GCGTTGATACCACCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT3030.10707129959115018No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCTGTCTCTTATACACAT3020.10671792896543682No Hit
TTTTTGCGTTGATACCACCCGTACTCTGCGTTGATACCCTGTCTCTTATAC2960.10459770521115662No Hit
GATACCACTGCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTC2910.10283085208258977No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAGAAAACTGTCTCTTATA2890.10212411083116305No Hit
TATCAACGCAGAGTACGGGCAGTGGTATCAACCTGTCTCTTATACACATCT2870.10141736957973632No Hit
ATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG2840.10035725770259621No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAACTGTCTCTTAT2840.10035725770259621No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC253.88333E-545.045
TCTCAAC250.002104431436.02
ATGCCGT352.8150406E-432.1428644
CCTGCTA352.8150406E-432.142861
GTAATAC300.005140780529.9999981
GTCGGTT300.005140780529.99999829
CTGCTAC406.15457E-428.1252
TGCTACT406.15457E-428.1253
TGTGTCG406.15457E-428.12526
ACGGGCG608.163062E-626.24999816
CATTTAG608.163062E-626.2499982
GTCTCAA450.001224270425.01
ATATACA450.001224270425.03
TCGTATG450.001224270425.040
GTCTTAT450.001224270425.032
TGTCGGT450.001224270425.028
AGCTACC551.3646462E-424.5454527
CGGGGGG851.8831633E-723.82352817
GCATTTA702.6937012E-522.5000021
GTGTCTT500.002260425422.530
Produced by FastQC (version 0.11.2)