FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_14.34100000005632.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_14.34100000005632.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500319
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG900.032.4986343
ATGCCGT950.030.78817444
ACACCGT300.005143059430.0017326
ACGCCTA453.48795E-529.99873521
AAACGCC453.48795E-529.99873519
GCGGGAT300.005145573529.9987353
TGCCGTC1001.8189894E-1229.24876645
CGCCTAA507.15251E-526.99886122
CTCGTAT1251.8189894E-1225.19893639
TCGTATG1251.8189894E-1225.19893640
GGTATCA5000.024.761331
ACCTATA551.3624552E-424.5566881
GCCCACG1201.6370905E-1124.37397218
CGAGCCC1450.023.27720615
AAAACGC702.699934E-522.4990518
CCCACGA1305.0931703E-1122.4990519
GTATCAA9650.022.1603971
AATCTCG955.521106E-721.3148936
TAGGACA955.521106E-721.314894
TCTCGTA1503.43789E-1019.49917638
Produced by FastQC (version 0.11.2)