##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 30h_13.34100000005625.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6016 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.09308510638298 38.0 38.0 38.0 32.0 38.0 2 36.100398936170215 38.0 38.0 38.0 32.0 38.0 3 36.10422207446808 38.0 38.0 38.0 32.0 38.0 4 36.24983377659574 38.0 38.0 38.0 32.0 38.0 5 36.29970079787234 38.0 38.0 38.0 32.0 38.0 6 38.355551861702125 40.0 38.0 40.0 38.0 40.0 7 38.102726063829785 40.0 38.0 40.0 38.0 40.0 8 38.01628989361702 40.0 38.0 40.0 32.0 40.0 9 38.146609042553195 40.0 38.0 40.0 38.0 40.0 10 38.092087765957444 40.0 38.0 40.0 38.0 40.0 11 38.13297872340426 40.0 38.0 40.0 38.0 40.0 12 37.823470744680854 40.0 38.0 40.0 32.0 40.0 13 38.00498670212766 40.0 38.0 40.0 32.0 40.0 14 37.83228058510638 40.0 38.0 40.0 32.0 40.0 15 37.963264627659576 40.0 38.0 40.0 32.0 40.0 16 37.73371010638298 40.0 38.0 40.0 32.0 40.0 17 37.584275265957444 40.0 38.0 40.0 32.0 40.0 18 37.548038563829785 40.0 38.0 40.0 32.0 40.0 19 37.6328125 40.0 38.0 40.0 32.0 40.0 20 37.73354388297872 40.0 38.0 40.0 32.0 40.0 21 37.785904255319146 40.0 38.0 40.0 32.0 40.0 22 37.89145611702128 40.0 38.0 40.0 32.0 40.0 23 37.889461436170215 40.0 38.0 40.0 32.0 40.0 24 37.810006648936174 40.0 38.0 40.0 32.0 40.0 25 37.6845079787234 40.0 38.0 40.0 32.0 40.0 26 37.582114361702125 40.0 38.0 40.0 32.0 40.0 27 37.5327460106383 40.0 38.0 40.0 32.0 40.0 28 37.626662234042556 40.0 38.0 40.0 32.0 40.0 29 37.71376329787234 40.0 38.0 40.0 32.0 40.0 30 37.624002659574465 40.0 38.0 40.0 32.0 40.0 31 37.83510638297872 40.0 38.0 40.0 32.0 40.0 32 37.61702127659574 40.0 38.0 40.0 32.0 40.0 33 37.681349734042556 40.0 38.0 40.0 32.0 40.0 34 37.68317819148936 40.0 38.0 40.0 32.0 40.0 35 37.70960771276596 40.0 38.0 40.0 32.0 40.0 36 37.65757978723404 40.0 38.0 40.0 32.0 40.0 37 37.6298204787234 40.0 38.0 40.0 32.0 40.0 38 37.59308510638298 40.0 38.0 40.0 32.0 40.0 39 37.46226728723404 40.0 38.0 40.0 32.0 40.0 40 37.51778590425532 40.0 38.0 40.0 32.0 40.0 41 37.464760638297875 40.0 38.0 40.0 32.0 40.0 42 37.427859042553195 40.0 38.0 40.0 32.0 40.0 43 37.603390957446805 40.0 38.0 40.0 32.0 40.0 44 37.52260638297872 40.0 38.0 40.0 32.0 40.0 45 37.683510638297875 40.0 38.0 40.0 32.0 40.0 46 37.367686170212764 40.0 38.0 40.0 32.0 40.0 47 37.40990691489362 40.0 38.0 40.0 32.0 40.0 48 37.335438829787236 40.0 38.0 40.0 32.0 40.0 49 37.150099734042556 40.0 38.0 40.0 32.0 40.0 50 37.09591090425532 38.0 38.0 40.0 32.0 40.0 51 36.539561170212764 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.2379389273920651 2201 2 -4.1363001148157394 2201 3 -2.302373971514598 2201 4 -0.307432194541569 2201 5 -0.3465713539932267 2201 6 -2.208884778416042 2201 7 0.4391395063270096 2201 8 -2.531065722471972 2201 9 -1.1576427045177056 2201 10 0.17576017732269378 2201 11 -2.027403325840801 2201 12 -0.7409034975441173 2201 13 0.6536140075202681 2201 14 0.6918589223276754 2201 15 0.9290229735542255 2201 16 1.389899596930853 2201 17 -2.4063811622405424 2201 18 0.967975123834492 2201 19 1.0703711652930465 2201 20 -2.619632537601305 2201 21 0.1389414838633698 2201 22 -0.3232367892524337 2201 23 -0.15228452103452383 2201 24 -0.043561603327212595 2201 25 -0.7076068462787077 2201 26 -1.4425725749944505 2201 27 -0.7431113591269778 2201 28 -0.12367489281550803 2201 29 -1.2277766070734586 2201 30 -2.5547379270035293 2201 31 -5.402861288017526 2201 32 -3.081634511322001 2201 33 -6.088953494422228 2201 34 0.1321499247280684 2201 35 1.7643283799533833 2201 36 1.5952106053668658 2201 37 1.0757201565795356 2201 38 -2.5053405795593306 2201 39 -2.078961750055491 2201 40 -0.0958665032883772 2201 41 1.4904242025335748 2201 42 1.8391705516705557 2201 43 0.1536575837357148 2201 44 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0.7504515449827949 2202 16 0.7113281683594224 2202 17 0.07576169490231166 2202 18 0.18226083812020732 2202 19 0.28465687957876185 2202 20 -2.994632537601305 2202 21 -1.878915658993776 2202 22 0.212477496461851 2202 23 -0.45585594960595444 2202 24 -4.204275889041497 2202 25 -2.1183211319929924 2202 26 -1.1390011464230199 2202 27 -0.2966827876984084 2202 28 0.28703939289877667 2202 29 1.4150805357836873 2202 30 -1.215452212717814 2202 31 -1.7957184308746719 2202 32 -0.40306308275057035 2202 33 -2.1425249229936583 2202 34 -1.6357072181290775 2202 35 -1.6285287629037626 2202 36 -0.22621796606170363 2202 37 -3.4242798434204644 2202 38 -0.30891200813076125 2202 39 -0.1325331786269217 2202 40 -0.0422950747169466 2202 41 1.3832813453907207 2202 42 0.8748848373848404 2202 43 1.8322290123071454 2202 44 -1.7693033095376762 2202 45 -3.7026606206293664 2202 46 -2.646117901196014 2202 47 -3.518827396561761 2202 48 -0.5851960973054702 2202 49 -0.5559435789904512 2202 50 0.7853603427822051 2202 51 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37 2.004291585150966 1102 38 1.5482308490121 1102 39 -2.1325331786269217 1102 40 0.2434192109973381 1102 41 1.6689956311050054 1102 42 -1.839400876900875 1102 43 -0.8820567019785699 1102 44 -2.055017595251961 1102 45 -1.8455177634865123 1102 46 -1.78897504405316 1102 47 -0.5188273965617611 1102 48 0.12908961698024513 1102 49 0.15834213529526409 1102 50 0.6425031999250663 1102 51 1.0128377698690159 2203 1 1.130796070249211 2203 2 -2.7434429719585935 2203 3 1.340483171342548 2203 4 0.1032820911727157 2203 5 1.7784286460067733 2203 6 0.23754379301252726 2203 7 -0.3465747793872751 2203 8 0.9510771346708822 2203 9 0.28878586691086383 2203 10 0.8186173201798397 2203 11 -0.15240332584080107 2203 12 0.9912393595987368 2203 13 1.5821854360916987 2203 14 0.7990017794705295 2203 15 1.8933086878399408 2203 16 0.9970424540737071 2203 17 0.790047409188027 2203 18 -0.24631059045122328 2203 19 -3.001057406135523 2203 20 0.5767960338272644 2203 21 1.121084341006224 2203 22 1.0696203536047122 2203 23 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46 1.1395963845182706 1103 47 1.7311726034382389 1103 48 1.4148039026945298 1103 49 1.7297707067238335 1103 50 1.678217485639351 1103 51 2.977123484154731 2204 1 1.3212722607254008 2204 2 1.4470332185175963 2204 3 1.5309593618187378 2204 4 0.4842344721250953 2204 5 1.111761979340109 2204 6 0.9994485549172936 2204 7 1.7169172841047882 2204 8 1.8717120553058066 2204 9 1.495135073260073 2204 10 1.231315732878251 2204 11 1.847596674159199 2204 12 1.6896520580114398 2204 13 1.9313917852980467 2204 14 1.2751922556610111 2204 15 1.5123563068875612 2204 16 1.4732329302641887 2204 17 1.7741743933150147 2204 18 2.2457529016122706 2204 19 1.9037044986263822 2204 20 1.7831452401764736 2204 21 2.0417192616411484 2204 22 1.7045409885253449 2204 23 1.3060488122988119 2204 24 1.6369939522283445 2204 25 1.9451709314990708 2204 26 1.9879829805611067 2204 27 1.9096664186508008 2204 28 1.556880662740049 2204 29 1.8277789484820985 2204 30 1.8480398507742493 2204 31 1.9026942675380312 2204 32 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0.7481050633394517 1214 25 0.6673931537212923 1214 26 1.7657607583388852 1214 27 1.465221974206358 1214 28 1.6679917738511563 1214 29 1.9388900595932057 1214 30 1.736928739663135 1214 31 2.0138053786491383 1214 32 0.8350321553446634 1214 33 1.7860465055777723 1214 34 1.8404832580614041 1214 35 1.847661713286719 1214 36 1.6785439387002015 1214 37 1.7423868232461999 1214 38 1.7863260871073336 1214 39 1.296038249944509 1214 40 -0.5541998366217129 1214 41 1.407090869200239 1214 42 2.0891705516705557 1214 43 1.070324250402379 1214 44 1.040220499986134 1214 45 -0.2859939539627021 1214 46 0.05626305118493491 1214 47 -0.9354940632284254 1214 48 0.4981372360278655 1214 49 2.2297707067238335 1214 50 1.928217485639351 1214 51 2.060456817488067 1215 1 0.4879389273920651 1215 2 1.1136998851842606 1215 3 0.19762602848540212 1215 4 0.817567805458431 1215 5 1.2784286460067733 1215 6 0.6661152215839579 1215 7 1.2724728396603453 1215 8 1.093934277528028 1215 9 0.8840239621489587 1215 10 1.1757601773226938 1215 11 1.5142633408258632 1215 12 -0.19923683087745303 1215 13 1.3202806741869324 1215 14 -0.39147441100566027 1215 15 -3.737643693112439 1215 16 0.30656626359751726 1215 17 -0.28138116224054244 1215 18 0.051308457167827726 1215 19 0.32037116529304654 1215 20 1.3387007957320307 1215 21 1.0972748171967055 1215 22 1.260096544080902 1215 23 1.3060488122988119 1215 24 0.9147717300061231 1215 25 1.000726487054628 1215 26 1.0990940916722138 1215 27 1.6318886408730222 1215 28 1.1679917738511563 1215 29 1.4388900595932057 1215 30 1.5702620729964707 1215 31 -0.23619462135086167 1215 32 1.3350321553446634 1215 33 1.4527131722444366 1215 34 1.8404832580614041 1215 35 1.6809950466200476 1215 36 2.01187727203353 1215 37 1.9090534899128713 1215 38 -0.6303405795593306 1215 39 1.296038249944509 1215 40 1.8624668300449585 1215 41 1.5737575358669105 1215 42 2.25583721833722 1215 43 1.2369909170690505 1215 44 1.3735538333194697 1215 45 1.464006046037298 1215 46 1.306263051184935 1215 47 1.4811726034382389 1215 48 1.4148039026945298 1215 49 1.2297707067238335 1215 50 0.7615508189726867 1215 51 0.31045681748806686 1212 1 0.6546055940587294 1212 2 0.11369988518426055 1212 3 1.5309593618187378 1212 4 0.4842344721250953 1212 5 0.4450953126734376 1212 6 0.33278188825062216 1212 7 -1.5053049381174333 1212 8 -2.2393990558053076 1212 9 -1.7270871489621484 1212 10 -0.5464620448995277 1212 11 -0.5968477702852439 1212 12 -1.199236830877453 1212 13 -2.401941548035289 1212 14 0.8307478112165612 1212 15 1.5123563068875612 1212 16 -1.7489892919580328 1212 17 -1.4480478289072067 1212 18 0.13464179050115632 1212 19 0.5703711652930465 1212 20 0.4498119068431379 1212 21 -1.8471696272477445 1212 22 -1.6287923448079908 1212 23 -2.8050622988123024 1212 24 -2.2518949366605483 1212 25 -1.499273512945372 1212 26 0.32131631389443527 1212 27 -1.534778025793642 1212 28 -0.33200822614884373 1212 29 -2.0611099404067943 1212 30 -2.0408490381146436 1212 31 0.23602760087135977 1212 32 0.057254377566884784 1212 33 -2.825064605533342 1212 34 -0.715072297494153 1212 35 -4.152338286713281 1212 36 -2.54367828352202 1212 37 -3.202057621198243 1212 38 -2.8803405795593306 1212 39 -1.148406194499934 1212 40 -1.359755392177263 1212 41 -0.5373535752442038 1212 42 -2.29971833721833 1212 43 -1.929675749597621 1212 44 -0.8486683889027518 1212 45 -0.7026606206293664 1212 46 0.3062630511849349 1212 47 0.3700614923271246 1212 48 -1.029640541749913 1212 49 -0.49245151549838795 1212 50 -0.7384491810273133 1212 51 -2.7173209602897117 1213 1 0.7022246416777804 1213 2 -0.02915725767288535 1213 3 0.4833403141996868 1213 4 0.1747106626012851 1213 5 0.064142931721058 1213 6 0.8089723644411038 1213 7 1.2248537920412943 1213 8 1.3796485632423128 1213 9 0.8602144383394403 1213 10 1.1043316058941244 1213 11 0.8475966741591989 1213 12 1.2769536453130286 1213 13 1.153614007520268 1213 14 1.2275732080419601 1213 15 -0.3209770264457745 1213 16 0.9970424540737071 1213 17 -0.28138116224054244 1213 18 0.6822608381202073 1213 19 1.5703711652930465 1213 20 0.6482246052558409 1213 21 -0.3789156589937761 1213 22 0.5696203536047122 1213 23 1.115572621822622 1213 24 0.9385812538156415 1213 25 -1.046892560564423 1213 26 -0.7818582892801658 1213 27 -0.2252542162698319 1213 28 -1.5701034642440774 1213 29 1.1293662500693955 1213 30 0.5702620729964707 1213 31 0.9185672834110434 1213 32 -1.188777368464855 1213 33 1.2860465055777723 1213 34 -0.1357072181290775 1213 35 -1.199957334332332 1213 36 0.13092489108115046 1213 37 -0.852851271991895 1213 38 0.6910879918692387 1213 39 -0.2753903214840605 1213 40 1.386276353854477 1213 41 -0.6881472260378558 1213 42 0.7320276945277016 1213 43 0.04651472659286071 1213 44 1.2306966904623238 1213 45 -0.059803477772220504 1213 46 1.0681678130897012 1213 47 0.19545831772395417 1213 48 0.3433753312659604 1213 49 -1.9845150075618818 1213 50 -0.6432110857892184 1213 51 1.1556949127261618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 2.0 22 4.0 23 7.0 24 8.0 25 22.0 26 32.0 27 41.0 28 59.0 29 81.0 30 107.0 31 124.0 32 137.0 33 179.0 34 199.0 35 292.0 36 336.0 37 501.0 38 960.0 39 2923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.732623877618888 14.250083139341536 11.090788160957764 52.92650482208181 2 10.871010638297872 12.815824468085108 58.06183510638297 18.25132978723404 3 12.450132978723403 13.87965425531915 46.90824468085106 26.761968085106385 4 7.679521276595745 39.26196808510639 29.886968085106385 23.17154255319149 5 8.793218085106384 25.299202127659576 56.28324468085106 9.62433510638298 6 18.633643617021274 56.13364361702128 15.126329787234042 10.106382978723403 7 15.80784574468085 21.27659574468085 23.055186170212767 39.860372340425535 8 15.558510638297873 54.97007978723404 13.164893617021276 16.306515957446805 9 14.311835106382977 12.948803191489361 13.87965425531915 58.85970744680851 10 10.32247340425532 52.3936170212766 18.25132978723404 19.03257978723404 11 18.75 17.852393617021274 44.132313829787236 19.26529255319149 12 12.649601063829788 22.440159574468087 22.872340425531913 42.037898936170215 13 16.42287234042553 11.63563829787234 53.00864361702128 18.93284574468085 14 13.181515957446807 12.450132978723403 19.98005319148936 54.38829787234043 15 14.511303191489361 17.237367021276594 23.138297872340424 45.11303191489361 16 41.39650872817955 15.99334995843724 16.408977556109726 26.201163757273484 17 18.849734042553195 42.902260638297875 15.990691489361703 22.257313829787233 18 48.88630319148936 20.3125 14.827127659574469 15.97406914893617 19 16.65558510638298 23.8530585106383 17.386968085106382 42.10438829787234 20 22.37367021276596 16.0405585106383 15.076462765957446 46.5093085106383 21 13.79654255319149 14.112367021276595 15.508643617021276 56.58244680851063 22 13.164893617021276 44.1156914893617 14.960106382978724 27.7593085106383 23 11.768617021276597 20.212765957446805 17.037898936170212 50.98071808510638 24 40.59175531914894 23.95279255319149 16.256648936170212 19.19880319148936 25 18.83311170212766 42.5531914893617 14.511303191489361 24.102393617021274 26 49.617686170212764 18.882978723404257 16.788563829787233 14.710771276595743 27 18.15159574468085 50.8311170212766 16.023936170212767 14.993351063829788 28 21.79188829787234 18.683510638297875 15.325797872340424 44.19880319148936 29 12.184175531914894 51.37965425531915 16.090425531914892 20.345744680851062 30 12.998670212765958 21.309840425531913 41.24002659574468 24.451462765957448 31 15.043218085106384 49.700797872340424 19.8969414893617 15.35904255319149 32 38.94614361702128 20.511968085106382 26.263297872340424 14.278590425531915 33 17.652925531914892 48.371010638297875 17.5531914893617 16.42287234042553 34 23.68683510638298 16.68882978723404 17.51994680851064 42.10438829787234 35 12.915558510638297 49.25199468085106 16.93816489361702 20.894281914893618 36 11.735372340425531 44.66422872340425 18.99933510638298 24.601063829787233 37 12.68284574468085 55.55186170212766 17.37034574468085 14.394946808510639 38 11.36968085106383 26.678856382978722 45.17952127659575 16.7719414893617 39 10.77127659574468 23.221409574468087 24.168882978723406 41.838430851063826 40 12.450132978723403 14.760638297872342 54.138962765957444 18.650265957446805 41 12.23404255319149 13.946143617021276 22.67287234042553 51.146941489361694 42 38.3311170212766 14.943484042553193 29.055851063829785 17.669547872340424 43 14.827127659574469 15.259308510638297 43.65026595744681 26.263297872340424 44 18.351063829787233 42.619680851063826 23.76994680851064 15.259308510638297 45 10.871010638297872 18.866356382978726 54.271941489361694 15.990691489361703 46 37.59973404255319 24.517952127659576 22.90558510638298 14.976728723404257 47 14.394946808510639 13.613696808510639 27.044547872340424 44.9468085106383 48 18.816489361702125 14.66090425531915 18.73337765957447 47.78922872340425 49 38.56382978723404 12.782579787234042 18.367686170212767 30.285904255319153 50 13.314494680851062 15.957446808510639 45.3623670212766 25.3656914893617 51 18.221113881961763 12.61845386533666 24.754779717373236 44.40565253532834 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 2.0 15 1.5 16 1.0 17 1.0 18 1.0 19 1.0 20 1.0 21 0.5 22 0.0 23 0.5 24 1.0 25 1.0 26 3.5 27 6.0 28 9.5 29 13.0 30 14.0 31 15.0 32 25.5 33 36.0 34 46.5 35 57.0 36 85.5 37 114.0 38 169.5 39 225.0 40 241.5 41 258.0 42 320.5 43 383.0 44 378.5 45 374.0 46 531.5 47 689.0 48 1632.0 49 2575.0 50 1521.0 51 467.0 52 429.5 53 392.0 54 301.0 55 210.0 56 152.0 57 94.0 58 68.0 59 42.0 60 31.5 61 21.0 62 16.5 63 12.0 64 11.5 65 11.0 66 7.5 67 4.0 68 2.5 69 1.0 70 2.0 71 3.0 72 3.5 73 4.0 74 2.5 75 0.5 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03324468085106383 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.016622340425531915 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.016622340425531915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 6016.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.46875 #Duplication Level Percentage of deduplicated Percentage of total 1 69.01254773595198 21.027260638297875 2 10.038188761593018 6.117021276595745 3 6.6012002182214955 6.033909574468085 4 5.073649754500818 6.183510638297872 5 2.673213311511184 4.072473404255319 6 1.745771958537916 3.1914893617021276 7 1.2547735951991272 2.6761968085106385 8 0.9274413529732678 2.2606382978723407 9 0.6001091107474086 1.6456117021276595 >10 1.9094380796508457 9.375 >50 0.0 0.0 >100 0.05455537370430987 3.8397606382978724 >500 0.05455537370430987 8.809840425531915 >1k 0.05455537370430987 24.767287234042552 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC 1490 24.767287234042552 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG 530 8.809840425531915 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT 231 3.8397606382978724 No Hit ATCCATTCCCTTCCATTCCATTTCATTCCATTCCATTCCATTCCATTCCAT 50 0.8311170212765957 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 34 0.5651595744680852 No Hit CACCAAAGCTTCGATTCCTCCAACCTGACACACTCTGGTCTTATTACTCAT 33 0.5485372340425532 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 30 0.49867021276595747 No Hit CCCAGCAATAATGCCTGTTGCAAAGAATTCTTTAGCATGGTCCGGGCCGGT 27 0.44880319148936176 No Hit AACCATGGTGGTGTCTGGAGGATTCACCTTCTTTGCCAATTATGTCAAAGA 21 0.3490691489361702 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 20 0.3324468085106383 No Hit TTGCTATCCCAGGCAGATACGCCCTCTGATACGCACTCAGATACAAAAGTC 19 0.31582446808510634 No Hit CTCAACCAGAAATCGAATTCCATATCCAACCATGTCTTTCCTCTCATCTCT 19 0.31582446808510634 No Hit TGTCACCACTGATGAAGACGCAGCGTTGAAGGATTATGTGGCATTGCATGG 18 0.2992021276595745 No Hit GAGCTACACACTCACCTATGAGCAAAGTCAGGCGACACAAAGATCAAGGCT 16 0.26595744680851063 No Hit CGCTTAAGGAGCTCACTATGGCAACTCATCAAAACAATAGTGGCAATCTGC 14 0.2327127659574468 No Hit AGACAGGCCCACATAGCATGCAAGTTTGCTGAGCTTAAAGAAAAGATCGAT 14 0.2327127659574468 No Hit GTAGTCCTGAAAGGAGCCTTCCAACTCCTCATTCCCTGACTACCTCATTCT 14 0.2327127659574468 No Hit CAGAATCGCTTCTGCGGCTTGCAGGTCTTTGCTGATTTTGGTTTTCTTTTT 14 0.2327127659574468 No Hit CAGTCAATGCATAATCGTCCGCATAGCCTTTTAACAGGTTCATGCTGCTAT 14 0.2327127659574468 No Hit GTAATGGAGGAAAGCATGTCTTTGCAGCCTGAAGTTTCAATACTGATGGTG 13 0.2160904255319149 No Hit TTGCATCTCCTATCATCGAATTACGCCGACGCATGTCGATGTGATTGACCC 12 0.19946808510638298 No Hit CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTC 12 0.19946808510638298 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 12 0.19946808510638298 No Hit ATGAAAAACCATGGATGGTTAATCTTAAGAGGGGACGAGAATCACCTGGAC 12 0.19946808510638298 No Hit CTAGTGTTTAGCCCAAGAGAAGTAGCAGAGAACTTGAAGCGTAGTGGTGCT 11 0.18284574468085107 No Hit GGTATATCACATGACCCATCACCAAACTCACGCGAACCTCAACTCCAGTCC 11 0.18284574468085107 No Hit ACCCTTCCTGGTCAGGGTTCCCAGCTGAACATTTGAAAATGGAAAAGATAT 11 0.18284574468085107 No Hit ACAAAAGCTGATTCGTGCCGTGTTTGGCACTAACAACGTAGATACCTGCGC 11 0.18284574468085107 No Hit GGTCTACCTGGTCACGATTGGATATCTGGGCTTGAAGTTGATACGCAATGA 11 0.18284574468085107 No Hit GATCCCAGTTCTGTTCTCCCTGGGCCTTATGGAGTATACGATCACCTTCTT 11 0.18284574468085107 No Hit ACCCTGAGGTAGTAAAAAAAGTCGCTGAAGCGCGAAGTAAATATGCCGAAA 10 0.16622340425531915 No Hit GCTCCAGAGCCTATGGGTGCTGATGACCTGTCTCTTATACACATCTCCGAG 10 0.16622340425531915 No Hit GGCCAAAATTGCGCGTGTTATATTCGCCAACACCTGATTGCGCACGTAACT 10 0.16622340425531915 No Hit CAACAATCATACTGTTCATACACCACGTTTCTCAAAACCTACCTCTTTTCT 10 0.16622340425531915 No Hit GTCTAAGTCCCTTGTTCACAGTCTTCTCCAATGAGGCTTTTCCTGGCTTTT 10 0.16622340425531915 No Hit GCAAAAATACAATGCCTTGCCCATGCTCAACAGCATCCAGATGAACATTGG 10 0.16622340425531915 No Hit ATGCAACAGCAATAAAGCTGTGAGTGGTTATTCCCCATCCCACCATAATTT 10 0.16622340425531915 No Hit ACGCTGAACACCAGTGTCAGCCAGGTGAATACCTGTCTCTTATACACATCT 10 0.16622340425531915 No Hit ATCAAAACGTTGTGGTGACAGTTGATGGTCAGCCTGTCTCTTATACACATC 9 0.14960106382978725 No Hit ATTCCATGTTGAGCAAGCTGGTTAGCTACCGCTAAATCTCGTGCCAACGCT 9 0.14960106382978725 No Hit CTCTTGTTTTTGTTGGGGGAAGGGAGACCAACTCCACAGAGACTGTCTCTT 9 0.14960106382978725 No Hit CCGCCTTGGTGATGGCCACCACGATAGGAACCTGTCTCTTATACACATCTC 9 0.14960106382978725 No Hit TACCTTAACTTCTTCGGATGGCAATACCCCGCGAATAATACTGCCCTGCAC 9 0.14960106382978725 No Hit GGCATTTGCAGCTGGTGCAAAATCATCAAGTGAAACGCAGGAATACGGCTA 9 0.14960106382978725 No Hit TGTGGGCGCTTTTGTACTGGGGGCAATTTTTACGCCACCGGATATTGTCTG 9 0.14960106382978725 No Hit AAATGCAAATCCACTTGCATCTATTTTCCTGAAGCAATTTACTGAGGTTTC 9 0.14960106382978725 No Hit GTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGC 9 0.14960106382978725 No Hit GTCTACCACAGAGAGTCCTCTAATGGAAGCTTCTCTCCCTGGAATCTGTTC 9 0.14960106382978725 No Hit TCTTTGGCTGGGATGCGCCAAACCGCATGCATTTTCAAGTGATCGATGAGG 9 0.14960106382978725 No Hit TTCCAGCTCAGGTAGTCTCTGTTTCAGAATTTGGCCTTCATCTACGCTGTC 8 0.13297872340425532 No Hit GGTCGGGAATCATTGTGTGTCAGCGAAAGTGAATCGTCGTTTGGTCCCATT 8 0.13297872340425532 No Hit GCCCTTGCCGCTGAGGCTAAACATAATGTGCAGTTTTCAACTGAGTCTGCT 8 0.13297872340425532 No Hit CCTCAGTGACCTCACTTTTTCTCCTGCTAAGGGGTCCCCTGGAAGACTCAT 8 0.13297872340425532 No Hit CCATAGTGGGTGATAGCCCCGTATGAGAAAAGTTTGGTGTGGAACTGAGTG 8 0.13297872340425532 No Hit CTCATGCTTTTCCTCATTGATGTCGGGGAATTGCATTTGCATGTTGATGGG 8 0.13297872340425532 No Hit CCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGT 8 0.13297872340425532 No Hit GGCTTGGTCATAGTTGGCATAGGCGGTGAGAGTGGGGGTAAACTCGTACTC 8 0.13297872340425532 No Hit GGTCAGGGGCCATACAGATGATTTTCAAGGTCGGAGACACCGTCGTTCTGT 8 0.13297872340425532 No Hit GTATCCAAAGGTCGACGAGGTTGTCACAATGATGTCATCACACCGAGAGAG 8 0.13297872340425532 No Hit ACATGGTGCCAAATGACAATCTGCGTTATCAGTATCGCATTTGGTCAGATG 8 0.13297872340425532 No Hit GAGTTATTGATCCTGAGTTTCCCTTCTTTGAGCTGTTACCCAGAGTCTGTC 8 0.13297872340425532 No Hit CACCCGCCGTGAGATCAAAAACCTCAACTCGTTCAAGTTTATGCAACAGGC 8 0.13297872340425532 No Hit GCCTGCAGATTCATTAACTGGTTGGTCCCAGACTGATAGATGGTGTTGGTA 8 0.13297872340425532 No Hit TGGCAGATCTGGCACATTGATATTGAGCAGAGTAGGCTCGGTAAATTCGCT 8 0.13297872340425532 No Hit GAGAGAAGGATACAGGTCGAGCAATTTTATGGTGTCGATGTTCCGACCGGT 8 0.13297872340425532 No Hit CGTCGTTACCGTGTGCAGCGGCAGTATCTGTCTCTTATACACATCTCCGAG 8 0.13297872340425532 No Hit GTAAATGCAATATTTCATCCCCCGCGCCATTTGTAACCAAGTTGTAACTAC 7 0.1163563829787234 No Hit GCCTTAGCTAGCTTGCACTGGCTTTAGGTCGAATTATCTTATCGTATTATC 7 0.1163563829787234 No Hit ACCTTATCATGCAGGGACTTAAATGCAATGTGATCAGAAGGTTAGACTTCA 7 0.1163563829787234 No Hit AAGCTCCTCATCTAGTTAAGCCAAAAAGTTACGCCCAAAGAGAGCACAAAA 7 0.1163563829787234 No Hit TTCCCAGTTGGGTCAAATCCCAGACTATGCACTCTCCATAGGTCCCATCAG 7 0.1163563829787234 No Hit TTGTCGAGGTTTGTTATTAGCATAGATTCGCTGGAGGCAGTTACCGGTCTC 7 0.1163563829787234 No Hit CAGATCTTCACGAGGTCGGATCTTATGTGGGTCTAGCGTTCACCTGTCTCT 7 0.1163563829787234 No Hit TCTCTTGGGTTAAAAGATGCTGCTGGTAAAAGGGATTCCTCAGTTTTGGGG 7 0.1163563829787234 No Hit CAAGCGTCATGTGCTCATTGGTAAATACCGCACTTGAGTACATCATCGAGT 7 0.1163563829787234 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 7 0.1163563829787234 No Hit CAATAATCTTCGAGATGCCGTCCTCGTTAAGGGCGGTGACTGTCTCTTATA 7 0.1163563829787234 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC 7 0.1163563829787234 No Hit ATAGCGTGTAGCAAAACATCCGCATCGGAATGCCCGTCTAAGCCACGTTCG 7 0.1163563829787234 No Hit ATACACTGACCGTTCCCAAACCGGCAGCCGTATTCATGATCTGTCTCTTAT 7 0.1163563829787234 No Hit ACTACCAGGTTACTCATCCTGATAGTTAGTCAGTTGCTACTGGTGCAAGAT 7 0.1163563829787234 No Hit CTCTGGAGTCAGTTGTTATCTTTGTCTATGGTTAGAGCTTGTTACTCTCTG 7 0.1163563829787234 No Hit ACCATGGACCGATTGGACCAAAGGTATCAAAGCTTTTACCCTTGCCCCATT 7 0.1163563829787234 No Hit AGACAGAAGCTGGGCTTTAAGAAGTCGGGCGAGGCAATACCGAACCTGTCT 7 0.1163563829787234 No Hit ATACGGCATGATTTTGACCAACGCCCGTGCCACTGTCTCTTATACACATCT 7 0.1163563829787234 No Hit GCCTTCAGCTTCCCAATTCTTCATCTGGTTCACAGACATACGCAATACGTT 7 0.1163563829787234 No Hit ACGCATGATAGAACGATGTATTTGACTCTGAATGCAGCATCTGTCTCTTAT 7 0.1163563829787234 No Hit ACCCAGATTTGCGGCGGAAAAATACACTGCAAGCCTATGGCTGTCTCTTAT 7 0.1163563829787234 No Hit CCTCATACTTGTCTTCACCCAGCTCTTTTTGCAGCAGATAAACCTCGTTTT 7 0.1163563829787234 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.016622340425531915 3 0.0 0.0 0.14960106382978725 4 0.0 0.0 0.41555851063829785 5 0.0 0.0 0.6815159574468085 6 0.0 0.0 0.7646276595744681 7 0.0 0.0 0.8477393617021277 8 0.0 0.0 0.964095744680851 9 0.0 0.0 1.3297872340425532 10 0.0 0.0 1.8617021276595744 11 0.0 0.0 1.961436170212766 12 0.0 0.0 2.0279255319148937 13 0.0 0.0 2.077792553191489 14 0.0 0.0 2.1941489361702127 15 0.0 0.0 2.293882978723404 16 0.0 0.0 2.4933510638297873 17 0.0 0.0 2.6263297872340425 18 0.0 0.0 2.8091755319148937 19 0.0 0.0 2.8922872340425534 20 0.0 0.0 2.9753989361702127 21 0.0 0.0 3.2247340425531914 22 0.0 0.0 3.390957446808511 23 0.0 0.0 3.690159574468085 24 0.0 0.0 3.773271276595745 25 0.0 0.0 3.9893617021276597 26 0.0 0.0 4.122340425531915 27 0.0 0.0 4.321808510638298 28 0.0 0.0 4.936835106382978 29 0.0 0.0 5.501994680851064 30 0.0 0.0 5.934175531914893 31 0.0 0.0 6.94813829787234 32 0.0 0.0 7.89561170212766 33 0.0 0.0 9.042553191489361 34 0.0 0.0 10.023271276595745 35 0.0 0.0 11.087101063829786 36 0.0 0.0 12.034574468085106 37 0.0 0.0 13.11502659574468 38 0.0 0.0 14.278590425531915 39 0.0 0.0 15.37566489361702 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 165 0.0 45.0 45 ATGCCGT 190 0.0 39.078945 44 TATGCCG 230 0.0 32.282608 43 GTATGCC 235 0.0 31.595745 42 ATCTCGT 235 0.0 31.595745 37 TCTCGTA 235 0.0 31.595745 38 TCGTATG 235 0.0 31.595745 40 CTCGTAT 235 0.0 31.595745 39 CGTATGC 240 0.0 30.937498 41 AGCCCAC 245 0.0 30.306122 17 ACAAATC 210 0.0 30.0 33 AATCTCG 225 0.0 30.0 36 ACATAGA 210 0.0 30.0 27 GACAAAT 210 0.0 30.0 32 ATAGACA 210 0.0 30.0 29 CATAGAC 210 0.0 30.0 28 CAAATCT 210 0.0 30.0 34 TAGACAA 210 0.0 30.0 30 GCCCACG 240 0.0 29.999998 18 CCACGAG 240 0.0 29.999998 20 >>END_MODULE