FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_13.34100000005625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_13.34100000005625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6016
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC149024.767287234042552No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCG5308.809840425531915No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGT2313.8397606382978724No Hit
ATCCATTCCCTTCCATTCCATTTCATTCCATTCCATTCCATTCCATTCCAT500.8311170212765957No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC340.5651595744680852No Hit
CACCAAAGCTTCGATTCCTCCAACCTGACACACTCTGGTCTTATTACTCAT330.5485372340425532No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC300.49867021276595747No Hit
CCCAGCAATAATGCCTGTTGCAAAGAATTCTTTAGCATGGTCCGGGCCGGT270.44880319148936176No Hit
AACCATGGTGGTGTCTGGAGGATTCACCTTCTTTGCCAATTATGTCAAAGA210.3490691489361702No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC200.3324468085106383No Hit
TTGCTATCCCAGGCAGATACGCCCTCTGATACGCACTCAGATACAAAAGTC190.31582446808510634No Hit
CTCAACCAGAAATCGAATTCCATATCCAACCATGTCTTTCCTCTCATCTCT190.31582446808510634No Hit
TGTCACCACTGATGAAGACGCAGCGTTGAAGGATTATGTGGCATTGCATGG180.2992021276595745No Hit
GAGCTACACACTCACCTATGAGCAAAGTCAGGCGACACAAAGATCAAGGCT160.26595744680851063No Hit
CGCTTAAGGAGCTCACTATGGCAACTCATCAAAACAATAGTGGCAATCTGC140.2327127659574468No Hit
AGACAGGCCCACATAGCATGCAAGTTTGCTGAGCTTAAAGAAAAGATCGAT140.2327127659574468No Hit
GTAGTCCTGAAAGGAGCCTTCCAACTCCTCATTCCCTGACTACCTCATTCT140.2327127659574468No Hit
CAGAATCGCTTCTGCGGCTTGCAGGTCTTTGCTGATTTTGGTTTTCTTTTT140.2327127659574468No Hit
CAGTCAATGCATAATCGTCCGCATAGCCTTTTAACAGGTTCATGCTGCTAT140.2327127659574468No Hit
GTAATGGAGGAAAGCATGTCTTTGCAGCCTGAAGTTTCAATACTGATGGTG130.2160904255319149No Hit
TTGCATCTCCTATCATCGAATTACGCCGACGCATGTCGATGTGATTGACCC120.19946808510638298No Hit
CATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTC120.19946808510638298No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC120.19946808510638298No Hit
ATGAAAAACCATGGATGGTTAATCTTAAGAGGGGACGAGAATCACCTGGAC120.19946808510638298No Hit
CTAGTGTTTAGCCCAAGAGAAGTAGCAGAGAACTTGAAGCGTAGTGGTGCT110.18284574468085107No Hit
GGTATATCACATGACCCATCACCAAACTCACGCGAACCTCAACTCCAGTCC110.18284574468085107No Hit
ACCCTTCCTGGTCAGGGTTCCCAGCTGAACATTTGAAAATGGAAAAGATAT110.18284574468085107No Hit
ACAAAAGCTGATTCGTGCCGTGTTTGGCACTAACAACGTAGATACCTGCGC110.18284574468085107No Hit
GGTCTACCTGGTCACGATTGGATATCTGGGCTTGAAGTTGATACGCAATGA110.18284574468085107No Hit
GATCCCAGTTCTGTTCTCCCTGGGCCTTATGGAGTATACGATCACCTTCTT110.18284574468085107No Hit
ACCCTGAGGTAGTAAAAAAAGTCGCTGAAGCGCGAAGTAAATATGCCGAAA100.16622340425531915No Hit
GCTCCAGAGCCTATGGGTGCTGATGACCTGTCTCTTATACACATCTCCGAG100.16622340425531915No Hit
GGCCAAAATTGCGCGTGTTATATTCGCCAACACCTGATTGCGCACGTAACT100.16622340425531915No Hit
CAACAATCATACTGTTCATACACCACGTTTCTCAAAACCTACCTCTTTTCT100.16622340425531915No Hit
GTCTAAGTCCCTTGTTCACAGTCTTCTCCAATGAGGCTTTTCCTGGCTTTT100.16622340425531915No Hit
GCAAAAATACAATGCCTTGCCCATGCTCAACAGCATCCAGATGAACATTGG100.16622340425531915No Hit
ATGCAACAGCAATAAAGCTGTGAGTGGTTATTCCCCATCCCACCATAATTT100.16622340425531915No Hit
ACGCTGAACACCAGTGTCAGCCAGGTGAATACCTGTCTCTTATACACATCT100.16622340425531915No Hit
ATCAAAACGTTGTGGTGACAGTTGATGGTCAGCCTGTCTCTTATACACATC90.14960106382978725No Hit
ATTCCATGTTGAGCAAGCTGGTTAGCTACCGCTAAATCTCGTGCCAACGCT90.14960106382978725No Hit
CTCTTGTTTTTGTTGGGGGAAGGGAGACCAACTCCACAGAGACTGTCTCTT90.14960106382978725No Hit
CCGCCTTGGTGATGGCCACCACGATAGGAACCTGTCTCTTATACACATCTC90.14960106382978725No Hit
TACCTTAACTTCTTCGGATGGCAATACCCCGCGAATAATACTGCCCTGCAC90.14960106382978725No Hit
GGCATTTGCAGCTGGTGCAAAATCATCAAGTGAAACGCAGGAATACGGCTA90.14960106382978725No Hit
TGTGGGCGCTTTTGTACTGGGGGCAATTTTTACGCCACCGGATATTGTCTG90.14960106382978725No Hit
AAATGCAAATCCACTTGCATCTATTTTCCTGAAGCAATTTACTGAGGTTTC90.14960106382978725No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGC90.14960106382978725No Hit
GTCTACCACAGAGAGTCCTCTAATGGAAGCTTCTCTCCCTGGAATCTGTTC90.14960106382978725No Hit
TCTTTGGCTGGGATGCGCCAAACCGCATGCATTTTCAAGTGATCGATGAGG90.14960106382978725No Hit
TTCCAGCTCAGGTAGTCTCTGTTTCAGAATTTGGCCTTCATCTACGCTGTC80.13297872340425532No Hit
GGTCGGGAATCATTGTGTGTCAGCGAAAGTGAATCGTCGTTTGGTCCCATT80.13297872340425532No Hit
GCCCTTGCCGCTGAGGCTAAACATAATGTGCAGTTTTCAACTGAGTCTGCT80.13297872340425532No Hit
CCTCAGTGACCTCACTTTTTCTCCTGCTAAGGGGTCCCCTGGAAGACTCAT80.13297872340425532No Hit
CCATAGTGGGTGATAGCCCCGTATGAGAAAAGTTTGGTGTGGAACTGAGTG80.13297872340425532No Hit
CTCATGCTTTTCCTCATTGATGTCGGGGAATTGCATTTGCATGTTGATGGG80.13297872340425532No Hit
CCCTGTCTCTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGT80.13297872340425532No Hit
GGCTTGGTCATAGTTGGCATAGGCGGTGAGAGTGGGGGTAAACTCGTACTC80.13297872340425532No Hit
GGTCAGGGGCCATACAGATGATTTTCAAGGTCGGAGACACCGTCGTTCTGT80.13297872340425532No Hit
GTATCCAAAGGTCGACGAGGTTGTCACAATGATGTCATCACACCGAGAGAG80.13297872340425532No Hit
ACATGGTGCCAAATGACAATCTGCGTTATCAGTATCGCATTTGGTCAGATG80.13297872340425532No Hit
GAGTTATTGATCCTGAGTTTCCCTTCTTTGAGCTGTTACCCAGAGTCTGTC80.13297872340425532No Hit
CACCCGCCGTGAGATCAAAAACCTCAACTCGTTCAAGTTTATGCAACAGGC80.13297872340425532No Hit
GCCTGCAGATTCATTAACTGGTTGGTCCCAGACTGATAGATGGTGTTGGTA80.13297872340425532No Hit
TGGCAGATCTGGCACATTGATATTGAGCAGAGTAGGCTCGGTAAATTCGCT80.13297872340425532No Hit
GAGAGAAGGATACAGGTCGAGCAATTTTATGGTGTCGATGTTCCGACCGGT80.13297872340425532No Hit
CGTCGTTACCGTGTGCAGCGGCAGTATCTGTCTCTTATACACATCTCCGAG80.13297872340425532No Hit
GTAAATGCAATATTTCATCCCCCGCGCCATTTGTAACCAAGTTGTAACTAC70.1163563829787234No Hit
GCCTTAGCTAGCTTGCACTGGCTTTAGGTCGAATTATCTTATCGTATTATC70.1163563829787234No Hit
ACCTTATCATGCAGGGACTTAAATGCAATGTGATCAGAAGGTTAGACTTCA70.1163563829787234No Hit
AAGCTCCTCATCTAGTTAAGCCAAAAAGTTACGCCCAAAGAGAGCACAAAA70.1163563829787234No Hit
TTCCCAGTTGGGTCAAATCCCAGACTATGCACTCTCCATAGGTCCCATCAG70.1163563829787234No Hit
TTGTCGAGGTTTGTTATTAGCATAGATTCGCTGGAGGCAGTTACCGGTCTC70.1163563829787234No Hit
CAGATCTTCACGAGGTCGGATCTTATGTGGGTCTAGCGTTCACCTGTCTCT70.1163563829787234No Hit
TCTCTTGGGTTAAAAGATGCTGCTGGTAAAAGGGATTCCTCAGTTTTGGGG70.1163563829787234No Hit
CAAGCGTCATGTGCTCATTGGTAAATACCGCACTTGAGTACATCATCGAGT70.1163563829787234No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC70.1163563829787234No Hit
CAATAATCTTCGAGATGCCGTCCTCGTTAAGGGCGGTGACTGTCTCTTATA70.1163563829787234No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC70.1163563829787234No Hit
ATAGCGTGTAGCAAAACATCCGCATCGGAATGCCCGTCTAAGCCACGTTCG70.1163563829787234No Hit
ATACACTGACCGTTCCCAAACCGGCAGCCGTATTCATGATCTGTCTCTTAT70.1163563829787234No Hit
ACTACCAGGTTACTCATCCTGATAGTTAGTCAGTTGCTACTGGTGCAAGAT70.1163563829787234No Hit
CTCTGGAGTCAGTTGTTATCTTTGTCTATGGTTAGAGCTTGTTACTCTCTG70.1163563829787234No Hit
ACCATGGACCGATTGGACCAAAGGTATCAAAGCTTTTACCCTTGCCCCATT70.1163563829787234No Hit
AGACAGAAGCTGGGCTTTAAGAAGTCGGGCGAGGCAATACCGAACCTGTCT70.1163563829787234No Hit
ATACGGCATGATTTTGACCAACGCCCGTGCCACTGTCTCTTATACACATCT70.1163563829787234No Hit
GCCTTCAGCTTCCCAATTCTTCATCTGGTTCACAGACATACGCAATACGTT70.1163563829787234No Hit
ACGCATGATAGAACGATGTATTTGACTCTGAATGCAGCATCTGTCTCTTAT70.1163563829787234No Hit
ACCCAGATTTGCGGCGGAAAAATACACTGCAAGCCTATGGCTGTCTCTTAT70.1163563829787234No Hit
CCTCATACTTGTCTTCACCCAGCTCTTTTTGCAGCAGATAAACCTCGTTTT70.1163563829787234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1650.045.045
ATGCCGT1900.039.07894544
TATGCCG2300.032.28260843
GTATGCC2350.031.59574542
ATCTCGT2350.031.59574537
TCTCGTA2350.031.59574538
TCGTATG2350.031.59574540
CTCGTAT2350.031.59574539
CGTATGC2400.030.93749841
AGCCCAC2450.030.30612217
ACAAATC2100.030.033
AATCTCG2250.030.036
ACATAGA2100.030.027
GACAAAT2100.030.032
ATAGACA2100.030.029
CATAGAC2100.030.028
CAAATCT2100.030.034
TAGACAA2100.030.030
GCCCACG2400.029.99999818
CCACGAG2400.029.99999820
Produced by FastQC (version 0.11.2)