FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_12.34100000005618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_12.34100000005618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538055
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG300.005145320729.9994415
TATGCCG702.8807335E-828.92803243
AATTTAG406.159167E-428.12709216
TGCCGTC755.628499E-826.99949645
GTATCAA11550.025.328911
CTCGTAT801.0518124E-725.3120339
CCCGTCC450.001225839624.9995378
TATACCG450.001225839624.9995375
CGGGGAA651.5209183E-524.2325717
CCACGAG851.8900937E-723.82308820
GGTATCA5400.023.3372361
CGATTAG500.002263300422.49958225
GGCGATT500.002263300422.49958223
ACGAGAC903.280802E-722.49958222
AACGCAG13300.021.4845646
TATCAAC13600.021.1760772
CAACGCA13500.021.1662715
ACGCAGA13450.021.0776777
CAGTCCG754.597452E-520.9996099
TCAACGC13700.020.8572774
Produced by FastQC (version 0.11.2)