FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_11.3410000000560b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_11.3410000000560b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429667
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATACG300.00514410529.99953336
TTGCGCC300.00514410529.99953331
TGCCGTC856.4501364E-926.47325945
TACCGCA851.8881838E-723.82315813
GTCCTAA602.4584628E-422.5075071
TATGCCG1003.627065E-822.50227243
ACCGCAA802.967965E-622.49965314
AGGACGG500.002262523622.49965123
CCTAACC500.002262523622.4996513
CCGTTTT754.5945726E-520.99967413
CTTCGTT550.003932941720.454239
GCCCACG1109.949326E-820.4542318
CGCAAGG908.2326205E-619.9996916
ATATTAG807.5295895E-519.694071
ACAGCAC1051.4414818E-619.2854163
CGGGGTG2750.018.8178917
GTTTCGG600.00650460118.74970821
GGTTTCG600.00650460118.74970820
TTCGGGG600.00650460118.74970823
TTTTGCG1851.6370905E-1118.242962
Produced by FastQC (version 0.11.2)