FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 30h_10.341000000055fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 30h_10.341000000055fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479241
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC5220.10892223328137618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG950.030.78890243
TGCCGTC950.030.78890245
TATATAT300.00514476629.9994415
GAATTCG406.1615225E-428.12448117
AATCTCG1200.026.24951236
CGTGCGT450.001225643824.9995379
TATATGA450.001225643824.9995372
CTCGTAT1405.456968E-1222.49958239
ACTAGCC500.002262942322.4995823
GCGTACT500.002262942322.49958212
TGCGTAC500.002262942322.49958211
GCCCACG1305.0931703E-1122.4995818
ATGCCGT1358.367351E-1121.66626444
CCACGAG1358.367351E-1121.66626420
CGCCTAA955.51885E-721.31539322
TCTCGTA1401.364242E-1020.8924738
CGAGACA1401.364242E-1020.8924723
ACGAGAC1401.364242E-1020.8924722
GTGCGTA550.003933663520.45416510
GTATTGG1452.1827873E-1020.1741451
Produced by FastQC (version 0.11.2)