FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_94.341000000067f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_94.341000000067f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC181.2186865267433988RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC100.6770480704129994RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC90.6093432633716994RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC60.4062288422477996No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC50.3385240352064997No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC40.2708192281651997No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC40.2708192281651997No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.2031144211238998No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC30.2031144211238998No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGAATATCTCGTATGCCGTCT30.2031144211238998RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC30.2031144211238998No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAA20.13540961408259986No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAA20.13540961408259986No Hit
ATTTATTACTGTGTTTCCATTGTCAAATTTTATGATCTTGGCCTTTCTTTC20.13540961408259986No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGCTGTCTCTT20.13540961408259986No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCAC20.13540961408259986No Hit
CTTATACACATCTCAGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC20.13540961408259986No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers