FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_93.341000000067ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_93.341000000067ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344721
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC6620.19203935936598002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGA250.002105299835.99918716
GTATACT401.5576514E-533.7492374
TATGCCG1350.033.3325843
ATGCCGT1400.032.1421344
GTATAGT453.4602806E-530.0298141
TGCCGTC1450.029.48209645
ACGAGAC1650.028.63571722
CCACGAG1650.028.63571720
GCCCACG1700.027.7934918
CCCACGA1700.027.7934919
CGAGCCC1700.027.7934915
CACGAGA1750.026.99939221
AATCTCG1159.094947E-1225.43420836
CGAGACC1251.8189894E-1225.1994323
CGAGACG450.001224974224.99943523
TGTCCCG450.001224974224.99943510
GTATCAA4750.024.656061
GTATAAT551.3563117E-424.5698491
GCGTAGA551.3563117E-424.5698491
CTCGTAT1750.024.4280239
Produced by FastQC (version 0.11.2)