FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_91.341000000067c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_91.341000000067c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340838
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGG356.2382933E-638.568792
TATGCCG301.1377129E-437.50293743
GCGAAAG300.005143959529.99794618
GATATAG507.132942E-527.0060751
ACGAGAC507.14721E-526.99815222
TCGTATG450.001225273224.99828740
ACTCCCA500.00226033822.5017611
TTACTAG602.4637123E-422.4984610
CACGAGA602.4637123E-422.4984621
GCCCACG500.002262266622.49845918
GTGTTAA654.223386E-420.7739051
CCACGAG654.231762E-420.76780720
CGCTAAT550.003925824520.4591481
TTAGATT550.00393250520.4531443
CCCACGA550.00393250520.45314419
TAAGGGT550.00393250520.4531444
ACGGGGT1009.022533E-720.2486133
GTATTGG1202.477991E-718.7542211
CAATTAT600.006492876418.7542211
CTGTATA600.006503889318.7487164
Produced by FastQC (version 0.11.2)