H23NVBCXX l02n01 24h_88.3410000000679a.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 24h_88.3410000000679a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12390
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	7182	57.96610169491525	No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT	248	2.0016142050040355	RNA PCR Primer, Index 44 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.7263922518159807	No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.4277643260694108	No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA	51	0.4116222760290557	No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.40355125100887806	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.37126715092816787	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	45	0.36319612590799033	No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	39	0.31476997578692495	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.23405972558514931	No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27	0.2179176755447942	No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA	23	0.18563357546408393	No Hit
GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.16142050040355124	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACA	20	0.16142050040355124	No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	19	0.15334947538337368	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG	19	0.15334947538337368	No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	17	0.13720742534301858	No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	16	0.12913640032284102	No Hit
GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16	0.12913640032284102	No Hit
ACTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	14	0.11299435028248588	No Hit
ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	14	0.11299435028248588	No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	14	0.11299435028248588	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	14	0.11299435028248588	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.10492332526230831	No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	13	0.10492332526230831	No Hit
CCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT	13	0.10492332526230831	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	25	3.670583E-5	45.0	45
TGCCGTC	795	0.0	43.584904	45
AATATCT	820	0.0	42.530487	34
GAGACAA	820	0.0	42.530487	24
ATCTCGT	815	0.0	42.51534	37
ATAATAT	815	0.0	42.51534	32
TCTCGTA	815	0.0	42.51534	38
TATCTCG	815	0.0	42.51534	36
ATGCCGT	815	0.0	42.51534	44
TAATATC	815	0.0	42.51534	33
ATATCTC	815	0.0	42.51534	35
CTCGTAT	815	0.0	42.51534	39
GACAACA	810	0.0	42.5	26
AGACAAC	810	0.0	42.5	25
CAACATA	805	0.0	42.484474	28
ACATAAT	805	0.0	42.484474	30
ACAACAT	805	0.0	42.484474	27
CATAATA	805	0.0	42.484474	31
AACATAA	805	0.0	42.484474	29
CGAGACA	825	0.0	42.272728	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content