##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_88.3410000000679a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12390 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.367877320419694 38.0 38.0 38.0 32.0 38.0 2 36.438418079096046 38.0 38.0 38.0 32.0 38.0 3 36.37619047619047 38.0 38.0 38.0 32.0 38.0 4 36.55310734463277 38.0 38.0 38.0 32.0 38.0 5 36.49733656174334 38.0 38.0 38.0 32.0 38.0 6 38.70266343825666 40.0 38.0 40.0 38.0 40.0 7 38.34471347861179 40.0 38.0 40.0 38.0 40.0 8 38.334301856335756 40.0 38.0 40.0 38.0 40.0 9 38.5545601291364 40.0 38.0 40.0 38.0 40.0 10 38.355609362389025 40.0 38.0 40.0 38.0 40.0 11 38.42566585956416 40.0 38.0 40.0 38.0 40.0 12 37.98732849071832 40.0 38.0 40.0 32.0 40.0 13 38.26876513317191 40.0 38.0 40.0 38.0 40.0 14 38.08079096045198 40.0 38.0 40.0 38.0 40.0 15 38.24818401937046 40.0 38.0 40.0 38.0 40.0 16 37.961662631154155 40.0 38.0 40.0 32.0 40.0 17 37.77562550443906 40.0 38.0 40.0 32.0 40.0 18 37.77974172719935 40.0 38.0 40.0 32.0 40.0 19 37.96666666666667 40.0 38.0 40.0 32.0 40.0 20 38.16142050040355 40.0 38.0 40.0 32.0 40.0 21 38.21678773204197 40.0 38.0 40.0 38.0 40.0 22 38.37118644067797 40.0 38.0 40.0 38.0 40.0 23 38.35326876513317 40.0 38.0 40.0 38.0 40.0 24 38.07594834543987 40.0 38.0 40.0 32.0 40.0 25 37.69491525423729 40.0 38.0 40.0 32.0 40.0 26 37.772477804681195 40.0 38.0 40.0 32.0 40.0 27 37.81541565778854 40.0 38.0 40.0 32.0 40.0 28 37.95964487489911 40.0 38.0 40.0 32.0 40.0 29 37.90815173527038 40.0 38.0 40.0 32.0 40.0 30 38.047054075867635 40.0 38.0 40.0 38.0 40.0 31 37.8681194511703 40.0 38.0 40.0 32.0 40.0 32 37.6862792574657 40.0 38.0 40.0 32.0 40.0 33 37.574253430185635 40.0 38.0 40.0 32.0 40.0 34 37.50734463276836 40.0 38.0 40.0 32.0 40.0 35 37.508555286521386 40.0 38.0 40.0 32.0 40.0 36 37.48062953995157 40.0 38.0 40.0 32.0 40.0 37 37.20080710250202 40.0 38.0 40.0 32.0 40.0 38 37.0226795803067 40.0 38.0 40.0 32.0 40.0 39 37.01291364003228 40.0 38.0 40.0 32.0 40.0 40 36.97352703793382 40.0 38.0 40.0 32.0 40.0 41 37.13478611783697 40.0 38.0 40.0 32.0 40.0 42 36.90104923325262 40.0 38.0 40.0 32.0 40.0 43 37.05181598062954 40.0 38.0 40.0 32.0 40.0 44 36.76085552865214 40.0 38.0 40.0 27.0 40.0 45 36.71985472154964 40.0 38.0 40.0 27.0 40.0 46 35.94414850686037 38.0 38.0 40.0 27.0 40.0 47 35.70177562550444 38.0 38.0 40.0 27.0 40.0 48 35.426150121065376 38.0 38.0 40.0 13.0 40.0 49 35.096206618240515 38.0 38.0 40.0 13.0 40.0 50 34.58966908797417 38.0 32.0 40.0 13.0 40.0 51 34.183212267958034 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.5461629462877582 2201 2 0.21061338754233816 2201 3 1.4008626945561247 2201 4 0.1665708158650716 2201 5 0.2572911219886507 2201 6 0.6781942267738401 2201 7 0.698257569044145 2201 8 0.377075288958153 2201 9 0.7765129347284727 2201 10 0.4175675353973176 2201 11 0.4910641814693619 2201 12 0.8279286662185683 2201 13 -2.336431236861422 2201 14 -0.10220113839097422 2201 15 1.3048207559476737 2201 16 1.2400490526987795 2201 17 -0.6127833832916636 2201 18 0.778717310322314 2201 19 -0.2209284504330995 2201 20 0.2367015672844417 2201 21 0.5054869107009878 2201 22 0.3635710449379914 2201 23 -0.428484695576401 2201 24 1.398392243927411 2201 25 0.9919411272550533 2201 26 -0.06037322994458094 2201 27 -0.7040511720869702 2201 28 0.7182300381727842 2201 29 0.6967987207474167 2201 30 0.7838003472001276 2201 31 0.9228915502106645 2201 32 -0.3301323044638309 2201 33 0.4804625840202732 2201 34 0.191048009513068 2201 35 -0.7228298418994967 2201 36 0.38648086749473265 2201 37 0.38441732545388874 2201 38 0.02101843749503729 2201 39 0.9020162339496025 2201 40 -0.06998085131210274 2201 41 -0.32566574227291056 2201 42 -0.2402731056234373 2201 43 -0.955162811777754 2201 44 1.798698159478164 2201 45 0.8327590096703545 2201 46 -1.2559423345430787 2201 47 -0.4246664008795591 2201 48 -0.16026584168421465 2201 49 1.266459217216891 2201 50 -0.09304626234573732 2201 51 0.3992290516395727 1101 1 1.7604486605734735 1101 2 0.9633606402895936 1101 3 0.9063572000506355 1101 4 0.9907466400408964 1101 5 0.15839002308754857 1101 6 0.3265458751254897 1101 7 1.5444114151979917 1101 8 1.0474049592878245 1101 9 0.5677217259372682 1101 10 1.0164686342984197 1101 11 -0.6682764778713022 1101 12 0.6685880068779042 1101 13 0.5866456862154976 1101 14 1.2824142462244126 1101 15 0.8213042724311919 1101 16 1.3279611406108671 1101 17 1.7113924408841612 1101 18 1.0589370905420878 1101 19 0.608741879237229 1101 20 1.110327940910821 1101 21 0.6538385590526374 1101 22 0.6437908251577653 1101 23 0.7198669527752486 1101 24 0.2885021340373015 1101 25 0.9754576107715351 1101 26 1.0769894074180542 1101 27 1.0761686081328037 1101 28 1.459988279931025 1101 29 1.4385569625056576 1101 30 1.3827014461012297 1101 31 1.615199242518358 1101 32 1.5434940691625485 1101 33 1.046396649954339 1101 34 1.0976414161064767 1101 35 1.4090382899686347 1101 36 1.5238435048573677 1101 37 1.719582160618728 1101 38 1.7902492067258038 1101 39 2.1822360141693764 1101 40 2.45749167616043 1101 41 2.2127957961886295 1101 42 1.9685181031677672 1101 43 2.1217602651453262 1101 44 2.375621236401244 1101 45 2.8712205481318946 1101 46 2.7385631599624105 1101 47 3.674234698021543 1101 48 1.565008883590508 1101 49 0.7829427337004091 1101 50 1.9564042871048102 1101 51 3.6794488318593466 2202 1 -3.025265625140811 2202 2 -1.0751008981719465 2202 3 -3.4562801625867294 2202 4 -1.1905720412777896 2202 5 -0.09985173515421053 2202 6 0.10676565534526361 2202 7 -2.444599573813001 2202 8 0.019932431815291807 2202 9 -0.3663442081286661 2202 10 -0.8681467503169671 2202 11 -0.5803643899592146 2202 12 -1.8149284766385776 2202 13 -0.12214552257570688 2202 14 0.3263702901804564 2202 15 -2.5523221011951875 2202 16 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0.8373717374965537 1102 2 0.9633606402895936 1102 3 0.44481873851216847 1102 4 0.9907466400408964 1102 5 0.6199284846260156 1102 6 0.9419304905101029 1102 7 0.7751806459672252 1102 8 -0.5679796560967958 1102 9 0.7215678797834215 1102 10 -0.7527621349323539 1102 11 0.2548004452056247 1102 12 1.4378187761086778 1102 13 0.04818414775396462 1102 14 0.5901065539167192 1102 15 0.28284273396965176 1102 16 1.3279611406108671 1102 17 1.172930902422621 1102 18 1.0589370905420878 1102 19 1.6856649561603092 1102 20 0.4180202486031277 1102 21 1.1153770205910973 1102 22 0.7207139020808455 1102 23 1.489097722006015 1102 24 1.6731175186526883 1102 25 1.4369960723100021 1102 26 1.5385278689565212 1102 27 1.5377070696712707 1102 28 1.7676805876233317 1102 29 1.7462492701979642 1102 30 1.536547599947383 1102 31 0.9998146271337447 1102 32 1.2358017614702348 1102 33 1.2002428038004993 1102 34 1.9437952622603234 1102 35 0.17826905919940117 1102 36 0.5238435048573677 1102 37 2.104197545234115 1102 38 1.6364030528796505 1102 39 2.6437744757078434 1102 40 2.6113378300065833 1102 41 1.7512573346501696 1102 42 0.1992873339370007 1102 43 0.6602218036068592 1102 44 -0.23976337898337619 1102 45 0.6404513173626611 1102 46 0.6616400830393374 1102 47 0.9819270057138496 1102 48 -1.3580680394864117 1102 49 -0.5247495739919046 1102 50 -1.8128264821259634 1102 51 -2.7820896296791133 2203 1 1.0104486605734735 2203 2 0.29989910182805346 2203 3 1.0794341231275553 2203 4 -0.04771489842064369 2203 5 1.5430054077029354 2203 6 0.6246227982024095 2203 7 1.073257569044145 2203 8 0.5377895746724377 2203 9 0.740798649014188 2203 10 0.8818532496830329 2203 11 0.9374927528979313 2203 12 0.3993572376471377 2203 13 0.4760687631385778 2203 14 1.0227988616090258 2203 15 0.46553504166195836 2203 16 1.4721919098416336 2203 17 1.297930902422621 2203 18 -0.926639832534832 2203 19 0.8683572638526158 2203 20 0.7545587101415876 2203 21 0.5769154821295572 2203 22 -2.4042860979191545 2203 23 0.1429438758521684 2203 24 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1.0974893303976216 1211 24 -0.12758178204661164 1211 25 0.978954114268042 1211 26 -0.34608751565886564 1211 27 -0.11963558767138949 1211 28 0.6662819862247318 1211 29 0.1903052142539039 1211 30 -0.13827757487779024 1211 31 0.331982459301571 1211 32 0.7672702929387683 1211 33 1.3960469996046925 1211 34 0.7235155419806034 1211 35 -0.7552973743670321 1211 36 -0.035597054583185184 1211 37 0.6636381046746678 1211 38 0.7937457102223107 1211 39 -0.10447727254391026 1211 40 0.16378538245413665 1211 41 1.5379706213634563 1211 42 0.1363502709999409 1211 43 1.908473551858613 1211 44 2.298698159478164 1211 45 1.1963953733067214 1211 46 1.270031691430944 1211 47 0.7441647679516095 1211 48 1.865708184289808 1211 49 1.7339916847493555 1211 50 0.1731875038880233 1211 51 -0.6981735457630265 1208 1 -0.3995513394265231 1208 2 -0.2151008981719471 1208 3 -0.8105658768724453 1208 4 0.5322851015793546 1208 5 -0.6569945922970675 1208 6 0.3296227982024078 1208 7 -0.5817424309558561 1208 8 -0.6172104253275634 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1.2527590096703562 1208 46 -0.14451376311451014 1208 47 0.9296193134061568 1208 48 1.2511627297443582 1208 49 0.31217350293117363 1208 50 -0.25590340520288635 1208 51 -0.6036280912175727 1209 1 0.4687819939068092 1209 2 1.1748991018280535 1209 3 0.5794341231275553 1209 4 1.4522851015793563 1209 5 0.2513387410362711 1209 6 0.7496227982024095 1209 7 -0.010075764289190658 1209 8 0.9127895746724377 1209 9 1.1574653156808523 1209 10 0.09018658301636862 1209 11 1.395826086231267 1209 12 0.2743572376471377 1209 13 0.8302354298052421 1209 14 0.5227988616090258 1209 15 0.46553504166195836 1209 16 1.3471919098416336 1209 17 0.9645975690892854 1209 18 -0.614139832534832 1209 19 0.9933572638526158 1209 20 -0.2037746231917481 1209 21 1.160248815462893 1209 22 0.17904723541418122 1209 23 0.10127720918550409 1209 24 -0.21470299416782268 1209 25 0.7895601748741043 1209 26 0.23724581767447006 1209 27 -0.5552416482774518 1209 28 1.2420395619823026 1209 29 0.42894157789027076 1209 30 1.0397527281525072 1209 31 1.4228915502106645 1209 32 1.312724838393315 1209 33 0.0816530602107548 1209 34 1.4470003904654476 1209 35 0.8545511104814523 1209 36 -2.5659000848862163 1209 37 0.40227446831103464 1209 38 0.3126851041617016 1209 39 2.0924924244257923 1209 40 1.0312096248783789 1209 41 0.09100092439375373 1209 42 0.9621078467575117 1209 43 1.3365038548889103 1209 44 1.048698159478164 1209 45 1.9160923430036902 1209 46 1.732152903552155 1209 47 1.872952646739492 1209 48 1.4944960630776905 1209 49 2.34384016959784 1209 50 3.2224299281304525 1209 51 1.798038575449091 1214 1 -0.2812180060931908 1214 2 -0.28343423150528224 1214 3 0.03776745646089097 1214 4 0.7022851015793563 1214 5 0.29300540770293537 1214 6 -0.4170438684642548 1214 7 -0.676742430955855 1214 8 -0.4622104253275623 1214 9 0.032465315680852314 1214 10 -0.7848134169836314 1214 11 0.395826086231267 1214 12 -0.058976095686197993 1214 13 -0.7947645701947579 1214 14 -1.6438678050576385 1214 15 -0.28446495833804164 1214 16 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0.019707919832733012 1215 2 0.12860280553175585 1215 3 0.718323012016441 1215 4 0.34117399046824204 1215 5 0.4318942965918211 1215 6 0.43480798338759286 1215 7 0.36492423571080934 1215 8 -0.8186919068090432 1215 9 -1.0647569065413691 1215 10 -0.10888749105770756 1215 11 -2.2523220619168853 1215 12 -0.3599020216121218 1215 13 -0.07717197760216266 1215 14 -0.36146039765023374 1215 15 0.4794239305508441 1215 16 0.4675622802120074 1215 17 -0.08632835683663842 1215 18 0.3580823896873895 1215 19 0.585949856445211 1215 20 -0.3797005491176719 1215 21 -0.08975118453710706 1215 22 -1.1681749868080402 1215 23 -0.4311301982219078 1215 24 -1.0248881793530131 1215 25 -0.16414352882960515 1215 26 -1.51275418232553 1215 27 -0.7728342408700399 1215 28 -0.14684932690658314 1215 29 -0.834947310998615 1215 30 -0.7426546792548976 1215 31 -1.2252565979374808 1215 32 -0.09468256901408978 1215 33 -0.39519879164109994 1215 34 -0.5576292391641786 1215 35 0.30362518455552845 1215 36 0.0822480632619289 1215 37 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1212 23 0.2858010187093143 1212 24 -0.7801791846440125 1212 25 -0.25805887274494665 1212 26 0.6539124843411344 1212 27 0.4745202564844533 1212 28 -0.3531985332557923 1212 29 0.9110844350331249 1212 30 0.9266574900572735 1212 31 -0.5056798783607661 1212 32 0.4912962669647456 1212 33 -1.0909659874082962 1212 34 0.9767622952273527 1212 35 1.2057415866719339 1212 36 1.0293380103518786 1212 37 -0.18701124597468066 1212 38 -0.6575529910763933 1212 39 0.4377305196638872 1212 40 1.1800191486878973 1212 41 0.6386199720128047 1212 42 0.6525840372337015 1212 43 1.687694331079392 1212 44 1.2629838737638792 1212 45 1.9756161525275004 1212 46 0.4583433797426366 1212 47 1.1467621705490103 1212 48 1.1968770154586394 1212 49 0.9093163600740297 1212 50 0.2640965947971168 1212 51 0.9706576230681421 1213 1 -2.810979910855096 1213 2 -1.6703389934100414 1213 3 -1.0753277816343498 1213 4 -2.8334291841349284 1213 5 -0.17128030658277993 1213 6 -1.3694248208452109 1213 7 -0.9207900500034754 1213 8 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-3.0822542214742157 1213 45 -3.8577171808058353 1213 46 -2.660704239304984 1213 47 -3.662761638974793 1213 48 -0.20788488930326565 1213 49 -3.1144931637354887 1213 50 -0.3549510242505036 1213 51 -1.9102947578842375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 3.0 22 8.0 23 16.0 24 29.0 25 36.0 26 70.0 27 102.0 28 135.0 29 172.0 30 234.0 31 267.0 32 357.0 33 377.0 34 501.0 35 584.0 36 780.0 37 1206.0 38 1808.0 39 5701.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.987079060001616 5.870952111766131 7.518371961560204 66.62359686667205 2 5.415657788539145 9.096045197740112 78.47457627118644 7.013720742534302 3 8.167877320419693 15.383373688458434 70.26634382566586 6.182405165456013 4 4.648910411622276 67.00564971751413 15.89184826472962 12.45359160613398 5 6.569814366424535 15.649717514124294 70.0726392251816 7.707828894269572 6 10.36319612590799 74.20500403551252 5.528652138821631 9.90314769975787 7 9.741727199354317 15.899919289749798 8.087167070217918 66.27118644067797 8 10.807102502017758 69.7094430992736 6.828087167070218 12.655367231638417 9 11.154156577885391 8.918482647296207 8.087167070217918 71.84019370460048 10 9.136400322841002 70.19370460048427 10.411622276029057 10.258272800645681 11 10.016142050040354 11.186440677966102 69.136400322841 9.661016949152543 12 11.62227602905569 10.153349475383374 12.760290556900728 65.46408393866021 13 10.944309927360775 9.76594027441485 70.75060532687651 8.53914447134786 14 8.732849071832122 9.37046004842615 13.075060532687651 68.82163034705407 15 9.07183212267958 10.718321226795803 13.01049233252623 67.19935431799838 16 65.95641646489105 14.778046811945117 11.267150928167876 7.998385794995964 17 8.724778046811945 70.2179176755448 10.66182405165456 10.3954802259887 18 68.73839696504965 9.161352812979255 12.850108967632579 9.250141254338526 19 9.281678773204197 9.49959644874899 13.325262308313155 67.89346246973366 20 7.958030669895076 7.861178369652946 13.704600484261501 70.47619047619048 21 7.449556093623891 11.638418079096045 14.180790960451978 66.73123486682809 22 6.682808716707021 69.59644874899112 14.430992736077483 9.289749798224374 23 8.393866020984666 11.541565778853915 14.285714285714285 65.77885391444713 24 66.09362389023406 13.454398708635997 14.447134786117838 6.004842615012106 25 8.006456820016142 71.54156577885391 14.689265536723164 5.762711864406779 26 65.23002421307505 15.117029862792574 14.600484261501212 5.052461662631154 27 5.117029862792575 72.42937853107345 14.907183212267958 7.54640839386602 28 4.810330912025827 15.439870863599678 13.656174334140436 66.09362389023406 29 4.3906376109765946 74.87489911218724 14.00322841000807 6.731234866828087 30 5.13317191283293 73.02663438256658 13.171912832929783 8.668280871670703 31 5.13317191283293 15.391444713478611 13.446327683615818 66.02905569007264 32 5.3591606133979015 72.33252623083132 15.722356739305893 6.585956416464891 33 5.181598062953995 14.83454398708636 73.74495560936238 6.238902340597256 34 5.189669087974172 75.181598062954 13.914447134786117 5.714285714285714 35 4.891041162227603 73.5593220338983 16.133979015334948 5.415657788539145 36 5.012106537530267 16.682808716707022 72.7683615819209 5.536723163841808 37 4.705407586763519 73.93866020984665 16.287328490718323 5.068603712671509 38 4.850686037126715 14.100080710250202 73.34140435835351 7.707828894269572 39 4.915254237288136 13.954802259887005 16.19047619047619 64.93946731234867 40 4.93139628732849 13.268765133171915 73.32526230831316 8.47457627118644 41 7.062146892655368 13.640032284100082 13.535108958837771 65.76271186440678 42 64.76997578692493 12.663438256658596 15.673930589184826 6.892655367231638 43 4.778046811945117 15.617433414043584 72.61501210653753 6.989507667473769 44 4.818401937046005 72.63115415657788 15.778853914447136 6.771589991928975 45 7.046004842615012 13.107344632768362 72.64729620661824 7.199354317998385 46 64.76190476190476 12.623083131557708 12.81678773204197 9.798224374495561 47 5.73042776432607 12.631154156577887 12.171105730427763 69.46731234866829 48 7.586763518966909 12.001614205004035 11.912832929782082 68.49878934624697 49 64.84261501210653 12.251815980629539 13.317191283292978 9.588377723970943 50 5.439870863599677 11.533494753833736 71.79983857949959 11.22679580306699 51 5.157801275324885 11.897651142142223 14.060860440713535 68.88368714181937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7.0 1 4.5 2 2.0 3 3.0 4 4.0 5 4.0 6 4.0 7 4.5 8 5.0 9 6.0 10 7.0 11 21.0 12 35.0 13 93.5 14 152.0 15 200.0 16 248.0 17 255.5 18 263.0 19 269.5 20 276.0 21 250.0 22 224.0 23 228.5 24 233.0 25 247.0 26 199.5 27 138.0 28 103.5 29 69.0 30 81.0 31 93.0 32 96.0 33 99.0 34 115.5 35 132.0 36 140.5 37 149.0 38 169.5 39 190.0 40 196.0 41 202.0 42 236.5 43 271.0 44 451.5 45 632.0 46 4082.5 47 7533.0 48 3895.0 49 257.0 50 188.0 51 119.0 52 120.0 53 121.0 54 102.5 55 84.0 56 74.5 57 65.0 58 67.5 59 70.0 60 67.0 61 64.0 62 65.0 63 66.0 64 60.5 65 55.0 66 59.0 67 63.0 68 53.5 69 44.0 70 47.5 71 51.0 72 39.0 73 27.0 74 29.0 75 28.0 76 25.0 77 18.5 78 12.0 79 8.0 80 4.0 81 2.5 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05649717514124294 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.008071025020177564 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.008071025020177564 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 12390.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.717514124293785 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79793590439978 27.280064568200164 2 4.263986963606736 2.5343018563357544 3 1.222161868549701 1.0895883777239708 4 0.7604562737642585 0.903954802259887 5 0.35306898424769145 0.5246166263115416 6 0.08147745790331341 0.14527845036319612 7 0.16295491580662683 0.3389830508474576 8 0.21727322107550243 0.5165456012913641 9 0.21727322107550243 0.5811138014527845 >10 0.7876154263986964 4.552058111380145 >50 0.08147745790331341 1.565778853914447 >100 0.027159152634437803 2.0016142050040355 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.027159152634437803 57.96610169491525 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 7182 57.96610169491525 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 248 2.0016142050040355 RNA PCR Primer, Index 44 (95% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.7263922518159807 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.4277643260694108 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 51 0.4116222760290557 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.40355125100887806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.37126715092816787 No Hit TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 45 0.36319612590799033 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.31476997578692495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.23405972558514931 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.2179176755447942 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 23 0.18563357546408393 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.16142050040355124 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACA 20 0.16142050040355124 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 19 0.15334947538337368 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG 19 0.15334947538337368 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 17 0.13720742534301858 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 16 0.12913640032284102 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.12913640032284102 No Hit ACTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 14 0.11299435028248588 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 14 0.11299435028248588 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.11299435028248588 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 14 0.11299435028248588 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.10492332526230831 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 13 0.10492332526230831 No Hit CCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT 13 0.10492332526230831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.008071025020177562 3 0.0 0.0 0.016142050040355124 4 0.0 0.0 0.03228410008071025 5 0.0 0.0 0.03228410008071025 6 0.0 0.0 0.03228410008071025 7 0.0 0.0 0.05649717514124294 8 0.0 0.0 0.05649717514124294 9 0.0 0.0 0.0645682001614205 10 0.0 0.0 0.07263922518159806 11 0.0 0.0 0.08071025020177562 12 0.0 0.0 0.08878127522195318 13 0.0 0.0 0.08878127522195318 14 0.0 0.0 0.09685230024213075 15 0.0 0.0 0.11299435028248588 16 0.0 0.0 0.14527845036319612 17 0.0 0.0 0.15334947538337368 18 0.0 0.0 0.17756255044390637 19 0.0 0.0 0.20177562550443906 20 0.0 0.0 0.2179176755447942 21 0.0 0.0 0.22598870056497175 22 0.0 0.0 0.22598870056497175 23 0.0 0.0 0.25020177562550444 24 0.0 0.0 0.30669895076674736 25 0.0 0.0 0.3309120258272801 26 0.0 0.0 0.37933817594834546 27 0.0 0.0 0.4035512510088781 28 0.0 0.0 0.48426150121065376 29 0.0 0.0 0.5488297013720742 30 0.0 0.0 0.6698950766747377 31 0.0 0.0 0.7828894269572235 32 0.0 0.0 1.0169491525423728 33 0.0 0.0 1.1138014527845037 34 0.0 0.0 1.2994350282485876 35 0.0 0.0 1.4527845036319613 36 0.0 0.0 1.7191283292978208 37 0.0 0.0 2.1065375302663436 38 0.0 0.0 2.477804681194512 39 0.0 0.0 2.857142857142857 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 25 3.670583E-5 45.0 45 TGCCGTC 795 0.0 43.584904 45 AATATCT 820 0.0 42.530487 34 GAGACAA 820 0.0 42.530487 24 ATCTCGT 815 0.0 42.51534 37 ATAATAT 815 0.0 42.51534 32 TCTCGTA 815 0.0 42.51534 38 TATCTCG 815 0.0 42.51534 36 ATGCCGT 815 0.0 42.51534 44 TAATATC 815 0.0 42.51534 33 ATATCTC 815 0.0 42.51534 35 CTCGTAT 815 0.0 42.51534 39 GACAACA 810 0.0 42.5 26 AGACAAC 810 0.0 42.5 25 CAACATA 805 0.0 42.484474 28 ACATAAT 805 0.0 42.484474 30 ACAACAT 805 0.0 42.484474 27 CATAATA 805 0.0 42.484474 31 AACATAA 805 0.0 42.484474 29 CGAGACA 825 0.0 42.272728 23 >>END_MODULE