FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_87.3410000000678d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_87.3410000000678d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155413
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC19161.2328440992709748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1700.041.0291245
ATGCCGT1850.038.9186444
TATGCCG2000.037.12473743
CCCACGA1950.036.92281319
TCGTATG1950.036.92281340
ATATCTC1650.036.81791735
ATAATAT1600.036.56223732
TAATATC1550.036.29006633
CCCTGAG250.002101591335.9997442
TCTCGTA1950.035.76897438
AATATCT1650.035.45429234
GCCCACG2050.035.121718
ATCTCGT1950.034.61513537
TATCTCG1700.034.4115236
CCACGAG2100.034.28546520
CTCGTAT2050.034.02414739
ACGAGAC2050.034.02414722
CGTATGC2150.033.48813241
CGAGCCC2150.033.48813215
CATAATA1800.032.49976731
Produced by FastQC (version 0.11.2)