FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_86.34100000006770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_86.34100000006770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504396
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAT952.1102096E-823.68337432
TCTCGTA1052.3592293E-923.57059738
TGCCGTC1052.3592293E-923.57059745
GTATCAA9300.022.503671
CATAATA1003.6354322E-822.49920831
CTCGTAT1104.0399755E-922.49920739
TATCTCG903.2804746E-722.49920736
TATGCCG1156.7484507E-921.5209843
CACGAGA1351.9572326E-919.99929621
GACCTAC1051.4414018E-619.28694711
TAAATAG706.9776876E-419.2850365
ACATAAT1051.4427715E-619.28503430
CCACGAG1302.7641363E-819.0377920
TATACCA951.31384095E-518.94675
CATCTCC16050.018.92456645
TCGTACA600.006506872418.7493382
AGGGGGC600.006506872418.7493388
TTACATA600.006506872418.7493384
AACGCAG11150.018.3625816
TACGGGC2350.018.19445415
Produced by FastQC (version 0.11.2)