FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_85.34100000006763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_85.34100000006763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344090
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC4330.12583916998459704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATC401.5577816E-533.748833
ATAATAT453.4846576E-529.99893432
CATAATA453.4846576E-529.99893431
TATGCCG1600.029.530243
TCTCGTA1700.029.11661338
TCGTATG1650.028.63534540
ATGCCGT1650.028.63534544
ACGAGAC1650.028.63534522
TGCCGTC1700.027.79716945
CTCGTAT1700.027.7931339
CGTATGC1750.026.99904341
CACGAGA1800.026.24906721
GCCCACG1850.025.53963318
CCCACGA1850.025.53963319
CGAGACT801.049666E-725.311623
ACGGCTA450.001224024125.00274524
AGACTAT450.00122506224.9991116
CGAGACG450.00122506224.99911123
AATCTCG651.5201413E-524.22990836
CCACGAG1950.024.22990820
Produced by FastQC (version 0.11.2)