FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_84.34100000006757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_84.34100000006757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28812
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC4361.513258364570318No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC560.1943634596695821No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC350.12147716229348883RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC290.10065250590031931No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG206.919225E-445.036
TGCCGTC850.045.045
CGAGACG206.919225E-445.023
GAGACGT206.919225E-445.024
ATGCCGT950.040.2631644
ATCTCGT1000.038.2537
CCACGAG1000.038.2520
TATGCCG1000.038.2543
CACGAGA1000.038.2521
TCTCGTA1000.038.2538
ACGAGAC1000.038.2522
CGTATGC1000.038.2541
TCGTATG1000.038.2540
CGAGACA601.4188117E-1037.50000423
CAACATA552.5556801E-936.8181828
ATAATAT552.5556801E-936.8181832
ACATAAT552.5556801E-936.8181830
TAATATC552.5556801E-936.8181833
CATAATA552.5556801E-936.8181831
AACATAA552.5556801E-936.8181829