FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_83.3410000000674a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_83.3410000000674a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115092
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC13591.1807944948389115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1400.045.00000445
ATGCCGT1550.040.6451644
GTGTATG301.1335488E-437.4999961
CGTATGC1700.037.05882341
TATGCCG1700.037.05882343
GGGCTTG250.002099253536.021
TCGTATG1750.036.040
CTCGTAT1700.035.73529439
TCTCGTA1700.035.73529438
CGAGACA1700.035.73529423
ATAATAT1450.035.6896632
ACATAAT1450.035.6896630
CCACGAG1800.035.00000420
ATCTCGT1750.034.71428737
ACGAGAC1950.034.61538322
TAATATC1500.034.50000433
ATATCTC1500.034.50000435
CATAATA1500.034.50000431
CGAGCCC1900.034.34210615
CCGAGCC1950.033.46153614
Produced by FastQC (version 0.11.2)