FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_82.3410000000673d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_82.3410000000673d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5951
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC450.7561754327003865No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC280.4705091581246849RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC240.40329356410687284RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC230.38648966560241976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1512350865400773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.13443118803562426No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC70.11762728953117123No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC60.10082339102671821No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC250.001943447936.045
ATGCCGT250.001943447936.044
TCTCCGA250.001943447936.011
AGCCCAC300.0047510529.99999817
ATCTCGT300.0047510529.99999837
ATCTCCG300.0047510529.99999810
GCCCACG300.0047510529.99999818
CCACGAG300.0047510529.99999820
TATGCCG300.0047510529.99999843
GAGCCCA300.0047510529.99999816
CACGAGA300.0047510529.99999821
CCCACGA300.0047510529.99999819
TCTCGTA300.0047510529.99999838
ACGAGAC300.0047510529.99999822
CGTATGC300.0047510529.99999841
CGAGCCC300.0047510529.99999815
CCGAGCC300.0047510529.99999814
TCGTATG300.0047510529.99999840
TCCGAGC300.0047510529.99999813
CTCGTAT300.0047510529.99999839
Produced by FastQC (version 0.11.2)