##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_82.3410000000673d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5951 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.66056125021005 38.0 32.0 38.0 32.0 38.0 2 35.75785582255083 38.0 32.0 38.0 32.0 38.0 3 35.71702234918501 38.0 32.0 38.0 32.0 38.0 4 35.86708116282978 38.0 32.0 38.0 32.0 38.0 5 35.98958158292724 38.0 32.0 38.0 32.0 38.0 6 37.759032095446145 40.0 38.0 40.0 32.0 40.0 7 37.57906234246345 40.0 38.0 40.0 32.0 40.0 8 37.51487145017644 40.0 38.0 40.0 32.0 40.0 9 37.551335909931105 40.0 38.0 40.0 32.0 40.0 10 37.47504621072089 38.0 38.0 40.0 32.0 40.0 11 37.47034111913964 38.0 38.0 40.0 32.0 40.0 12 37.37993614518568 38.0 38.0 40.0 32.0 40.0 13 37.45572172744077 38.0 38.0 40.0 32.0 40.0 14 37.215089900857 38.0 38.0 40.0 32.0 40.0 15 37.26802218114602 38.0 38.0 40.0 32.0 40.0 16 37.35405814148883 38.0 38.0 40.0 32.0 40.0 17 37.22063518736347 38.0 38.0 40.0 32.0 40.0 18 37.31641740883885 38.0 38.0 40.0 32.0 40.0 19 37.30482271887078 38.0 38.0 40.0 32.0 40.0 20 37.22819694169047 38.0 38.0 40.0 32.0 40.0 21 37.23844731977819 38.0 38.0 40.0 32.0 40.0 22 37.403629642076965 38.0 38.0 40.0 32.0 40.0 23 37.2932280289027 38.0 38.0 40.0 32.0 40.0 24 37.26903041505629 38.0 38.0 40.0 32.0 40.0 25 37.31440094101831 38.0 38.0 40.0 32.0 40.0 26 37.17576877835658 38.0 38.0 40.0 32.0 40.0 27 37.16165350361284 38.0 38.0 40.0 32.0 40.0 28 37.10015123508654 38.0 38.0 40.0 32.0 40.0 29 37.21307343303646 38.0 38.0 40.0 32.0 40.0 30 37.079314400941016 38.0 38.0 40.0 32.0 40.0 31 37.08233910267182 38.0 38.0 40.0 32.0 40.0 32 37.03142329020333 38.0 38.0 40.0 32.0 40.0 33 37.08822046714838 38.0 38.0 40.0 32.0 40.0 34 36.97328180137792 38.0 38.0 40.0 32.0 40.0 35 36.965047891110736 38.0 38.0 40.0 32.0 40.0 36 36.91782893631323 38.0 38.0 40.0 32.0 40.0 37 37.035288186859354 38.0 38.0 40.0 32.0 40.0 38 36.986556881196435 38.0 38.0 40.0 32.0 40.0 39 36.885901529154765 38.0 38.0 40.0 32.0 40.0 40 36.98101159468997 38.0 38.0 40.0 32.0 40.0 41 36.89900856998824 38.0 38.0 40.0 32.0 40.0 42 36.95429339606789 38.0 38.0 40.0 32.0 40.0 43 37.013443118803565 38.0 38.0 40.0 32.0 40.0 44 36.85683078474206 38.0 38.0 40.0 32.0 40.0 45 36.85195765417577 38.0 38.0 40.0 32.0 40.0 46 36.7373550663754 38.0 38.0 40.0 32.0 40.0 47 36.82053436397244 38.0 38.0 40.0 32.0 40.0 48 36.75449504284994 38.0 38.0 40.0 32.0 40.0 49 36.63703579230381 38.0 38.0 40.0 32.0 40.0 50 36.632498739707614 38.0 38.0 40.0 32.0 40.0 51 36.102503780877164 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.8935808982683966 2201 2 0.13033910533910387 2201 3 -1.0689078282828248 2201 4 1.1647890737734414 2201 5 0.6154243326118305 2201 6 0.7223992153679646 2201 7 1.0143804112554164 2201 8 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1.7206213924963834 1208 19 0.7634909361471927 1208 20 2.1591895517676676 1208 21 1.8616364538239623 1208 22 2.022115124458871 1208 23 1.4738478535353394 1208 24 0.8911323051948017 1208 25 1.4479414682539726 1208 26 -1.9549197330447114 1208 27 1.11698006854256 1208 28 1.269906655844153 1208 29 1.9188215863997087 1208 30 1.7430544282106837 1208 31 2.358311350108224 1208 32 -0.24287405303031306 1208 33 -0.4884672619047521 1208 34 1.1175769976551067 1208 35 1.7725671897546746 1208 36 1.7024469020562876 1208 37 -0.3400196158008555 1208 38 0.3003765331890307 1208 39 2.309763933982687 1208 40 1.3402732683982919 1208 41 0.45441806457430545 1208 42 1.2342284451659467 1208 43 2.0144486156204877 1208 44 1.961928436147204 1208 45 -0.1449861336580156 1208 46 1.6476060831529509 1208 47 1.0719877344877418 1208 48 1.8016121031745982 1208 49 0.3334347943722946 1208 50 2.2703085542929173 1208 51 1.8903409090909093 1209 1 -2.5508635461760463 1209 2 0.9081168831168824 1209 3 0.4866477272727323 1209 4 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2.0597346230158706 1215 13 1.5913696112914835 1215 14 1.374780731421346 1215 15 0.5182505862193381 1215 16 0.27064901244587247 1215 17 -1.3432827155483409 1215 18 -0.5793786075036138 1215 19 -0.9365090638528031 1215 20 0.8591895517676704 1215 21 1.8616364538239623 1215 22 -0.27788487554112606 1215 23 1.2738478535353366 1215 24 1.6911323051947988 1215 25 2.047941468253974 1215 26 1.5450802669552886 1215 27 1.7169800685425614 1215 28 1.4699066558441558 1215 29 -1.7811784136002942 1215 30 2.1430544282106894 1215 31 1.7583113501082224 1215 32 -2.742874053030313 1215 33 -2.7884672619047564 1215 34 -1.782423002344892 1215 35 -1.2274328102453254 1215 36 0.802446902056289 1215 37 1.759980384199146 1215 38 -1.3996234668109722 1215 39 0.4097639339826884 1215 40 1.7402732683982904 1215 41 2.154418064574301 1215 42 1.834228445165948 1215 43 1.6144486156204891 1215 44 -0.33807156385280024 1215 45 2.3550138663419844 1215 46 2.0476060831529566 1215 47 -0.8280122655122568 1215 48 -1.998387896825399 1215 49 -2.9665652056277025 1215 50 -0.6296914457070883 1215 51 -1.3096590909090864 1212 1 -3.8841968795093784 1212 2 -3.980772005772007 1212 3 -3.068907828282825 1212 4 -2.946322037337673 1212 5 -0.7179090007214981 1212 6 -0.610934117965364 1212 7 -2.318952922077912 1212 8 -0.6943288915945232 1212 9 -2.860998940295815 1212 10 -4.27910973575036 1212 11 -3.807183441558429 1212 12 -5.4291542658730165 1212 13 -0.14196372204185082 1212 14 -0.9807748241342082 1212 15 -0.12619385822510765 1212 16 -1.5293509875541247 1212 17 -0.24328271554833947 1212 18 -3.9682674963924995 1212 19 -6.636509063852806 1212 20 -2.1296993371212167 1212 21 -3.0939191017315935 1212 22 -3.8223293199855704 1212 23 0.02940340909089656 1212 24 -2.7755343614718626 1212 25 -1.4631696428571388 1212 26 0.5673024891775071 1212 27 -0.9052421536796587 1212 28 0.9810177669552687 1212 29 -2.5034006358225156 1212 30 -2.5013900162337563 1212 31 -0.2639108721139962 1212 32 1.0793481691919098 1212 33 -1.744022817460312 1212 34 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1.113490936147194 1213 20 2.009189551767669 1213 21 -2.4133635461760363 1213 22 0.24711512445887251 1213 23 -3.4011521464646606 1213 24 -0.4838676948051983 1213 25 -2.2270585317460245 1213 26 1.3450802669552857 1213 27 0.8169800685425628 1213 28 1.1199066558441544 1213 29 -2.156178413600294 1213 30 0.5680544282106865 1213 31 1.7083113501082252 1213 32 1.1071259469696884 1213 33 0.31153273809524507 1213 34 0.4425769976551095 1213 35 1.0225671897546746 1213 36 -1.6725530979437124 1213 37 -4.8400196158008555 1213 38 -1.3246234668109693 1213 39 0.7847639339826884 1213 40 0.11527326839829044 1213 41 -1.6205819354256974 1213 42 -3.040771554834052 1213 43 -6.260551384379511 1213 44 -2.238071563852799 1213 45 -3.794986133658014 1213 46 -2.5273939168470463 1213 47 -4.428012265512258 1213 48 -2.373387896825399 1213 49 -4.4665652056277025 1213 50 -5.6796914457070855 1213 51 -2.7596590909090892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 3.0 23 9.0 24 22.0 25 32.0 26 40.0 27 62.0 28 97.0 29 89.0 30 120.0 31 152.0 32 178.0 33 189.0 34 275.0 35 332.0 36 421.0 37 578.0 38 1008.0 39 2340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.3109243697479 17.3781512605042 12.756302521008402 30.554621848739494 2 15.425978827087885 20.51756007393715 38.36330028566628 25.693160813308687 3 18.71954293396068 22.954125357082845 30.179801713997644 28.14652999495883 4 14.199294236262814 18.853974121996302 34.6328348176777 32.31389682406318 5 14.871450176440934 35.069736178793484 36.43085195765418 13.627961687111409 6 32.868425474710136 37.4222819694169 15.375567131574524 14.333725424298438 7 28.381784574021175 30.465467988573348 20.315913291883717 20.83683414552176 8 24.70173080154596 38.245672996135106 19.122836498067553 17.929759704251385 9 24.14720215089901 17.341623256595533 19.44211056965216 39.0690640228533 10 15.997311376239287 30.347840699042177 31.708956477902873 21.94589144681566 11 33.708620399932784 22.399596706435894 24.752142497059317 19.139640396572005 12 22.416400604940346 23.71030078978323 29.877331540917496 23.99596706435893 13 27.894471517392034 21.10569652159301 27.457570156276255 23.542261804738697 14 21.156108217106368 20.719206855990592 26.247689463955638 31.876995462947406 15 22.601243488489327 28.331372878507814 23.861535876323305 25.20584775667955 16 24.466476222483617 28.583431356074605 24.348848932952443 22.601243488489327 17 22.130734330364646 30.011762728953116 25.911611493866577 21.94589144681566 18 24.634515207528146 27.306335069736175 26.919845404133756 21.139304318601916 19 23.239791631658544 26.214081666946733 25.70996471181314 24.83616198958158 20 22.76928247353386 27.087884389178292 25.978827087884387 24.16400604940346 21 22.55083179297597 26.26449336246009 26.617375231053604 24.567299613510336 22 21.72744076625777 28.465804066543438 26.55015963703579 23.256595530163 23 22.130734330364646 25.693160813308687 26.80221811460259 25.37388674172408 24 24.735338598554865 27.27272727272727 26.14686607292892 21.845068055788943 25 22.853301966056126 28.70105864560578 26.8694337086204 21.576205679717695 26 25.65955301629978 26.19727776844228 26.26449336246009 21.878675852797848 27 21.72744076625777 29.877331540917496 26.718198622080326 21.677029070744414 28 22.937321458578392 26.230885565451185 25.508317929759706 25.32347504621072 29 22.970929255587297 27.27272727272727 27.457570156276255 22.298773315409175 30 23.878339774827758 27.52478575029407 26.718198622080326 21.878675852797848 31 23.962359267350024 25.340278944715173 27.13829608469165 23.55906570324315 32 22.584439589984875 27.810452024869768 26.41572844900017 23.189379936145187 33 23.693496891278777 25.978827087884387 29.23878339774828 21.088892623088558 34 23.340615022685263 26.214081666946733 27.709628633843053 22.73567467652495 35 22.4332045034448 25.911611493866577 29.10435220971265 22.55083179297597 36 23.475046210720887 25.071416568643922 27.625609141320783 23.8279280793144 37 22.181146025878004 27.35674676524954 27.52478575029407 22.937321458578392 38 22.601243488489327 23.65988909426987 30.4822718870778 23.256595530163 39 21.77785246177113 23.592673500252058 28.5498235590657 26.07965047891111 40 21.626617375231053 24.634515207528146 31.372878507813812 22.36598890942699 41 22.013107040833475 23.122164342127373 28.364980675516723 26.499747941522433 42 23.273399428667453 25.357082843219626 28.465804066543438 22.903713661569483 43 20.61838346496387 24.38245672996135 31.42329020332717 23.575869601747605 44 20.584775667954965 26.75180641908923 28.768274239623594 23.895143673332214 45 20.215089900857 24.197613846412366 31.0199966392203 24.567299613510336 46 22.584439589984875 25.155436061166192 28.364980675516723 23.895143673332214 47 20.097462611325827 24.819358091077127 28.717862544110233 26.365316753486812 48 19.895815829272394 25.357082843219626 28.062510502436567 26.684590825071417 49 21.878675852797848 24.852965888086036 29.42362628129726 23.844731977818853 50 19.862208032263485 24.66812300453705 29.81011594689968 25.65955301629978 51 18.971601411527477 25.30667114770627 27.995294908418757 27.726432532347506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 1.5 2 1.0 3 0.5 4 0.0 5 0.5 6 1.0 7 2.0 8 3.0 9 2.0 10 1.0 11 4.0 12 7.0 13 13.0 14 19.0 15 18.0 16 17.0 17 15.5 18 14.0 19 13.5 20 13.0 21 10.5 22 8.0 23 8.5 24 9.0 25 13.5 26 18.5 27 19.0 28 31.5 29 44.0 30 65.5 31 87.0 32 117.5 33 148.0 34 196.5 35 245.0 36 294.0 37 343.0 38 378.5 39 414.0 40 457.5 41 501.0 42 489.0 43 477.0 44 459.5 45 442.0 46 459.0 47 476.0 48 486.5 49 497.0 50 458.0 51 419.0 52 434.0 53 449.0 54 395.5 55 342.0 56 301.5 57 261.0 58 238.5 59 216.0 60 184.0 61 152.0 62 126.0 63 100.0 64 88.5 65 77.0 66 64.0 67 51.0 68 41.0 69 31.0 70 24.5 71 18.0 72 15.0 73 12.0 74 10.0 75 5.5 76 3.0 77 2.5 78 2.0 79 1.0 80 0.0 81 0.5 82 1.0 83 1.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016803898504453033 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 5951.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.60561250210048 #Duplication Level Percentage of deduplicated Percentage of total 1 99.02591755087842 95.66459418585112 2 0.7305618368411898 1.4115274743740547 3 0.034788658897199516 0.10082339102671821 4 0.034788658897199516 0.13443118803562426 5 0.034788658897199516 0.16803898504453033 6 0.017394329448599758 0.10082339102671821 7 0.017394329448599758 0.11762728953117123 8 0.017394329448599758 0.13443118803562426 9 0.017394329448599758 0.1512350865400773 >10 0.06957731779439903 2.016467820534364 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 45 0.7561754327003865 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 28 0.4705091581246849 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 24 0.40329356410687284 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 23 0.38648966560241976 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.1512350865400773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.13443118803562426 No Hit CTTATACACATCTCCGAGCCCACGAGACTCTGGACCATCTCGTATGCCGTC 7 0.11762728953117123 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC 6 0.10082339102671821 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.016803898504453033 10 0.0 0.0 0.016803898504453033 11 0.0 0.0 0.016803898504453033 12 0.0 0.0 0.016803898504453033 13 0.0 0.0 0.016803898504453033 14 0.0 0.0 0.016803898504453033 15 0.0 0.0 0.016803898504453033 16 0.0 0.0 0.016803898504453033 17 0.0 0.0 0.016803898504453033 18 0.0 0.0 0.016803898504453033 19 0.0 0.0 0.016803898504453033 20 0.0 0.0 0.016803898504453033 21 0.0 0.0 0.016803898504453033 22 0.0 0.0 0.0504116955133591 23 0.0 0.0 0.0504116955133591 24 0.0 0.0 0.06721559401781213 25 0.0 0.0 0.06721559401781213 26 0.0 0.0 0.06721559401781213 27 0.0 0.0 0.1008233910267182 28 0.0 0.0 0.16803898504453033 29 0.0 0.0 0.28566627457570154 30 0.0 0.0 0.4200974626113258 31 0.0 0.0 0.9242144177449169 32 0.0 0.0 1.49554696689632 33 0.0 0.0 2.3189379936145187 34 0.0 0.0 3.007897832297093 35 0.0 0.0 3.7304654679885734 36 0.0 0.0 4.65467988573349 37 0.0 0.0 5.629305998991766 38 0.0 0.0 6.872794488321291 39 0.0 0.0 8.183498571668627 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 25 0.0019434479 36.0 45 ATGCCGT 25 0.0019434479 36.0 44 TCTCCGA 25 0.0019434479 36.0 11 AGCCCAC 30 0.00475105 29.999998 17 ATCTCGT 30 0.00475105 29.999998 37 ATCTCCG 30 0.00475105 29.999998 10 GCCCACG 30 0.00475105 29.999998 18 CCACGAG 30 0.00475105 29.999998 20 TATGCCG 30 0.00475105 29.999998 43 GAGCCCA 30 0.00475105 29.999998 16 CACGAGA 30 0.00475105 29.999998 21 CCCACGA 30 0.00475105 29.999998 19 TCTCGTA 30 0.00475105 29.999998 38 ACGAGAC 30 0.00475105 29.999998 22 CGTATGC 30 0.00475105 29.999998 41 CGAGCCC 30 0.00475105 29.999998 15 CCGAGCC 30 0.00475105 29.999998 14 TCGTATG 30 0.00475105 29.999998 40 TCCGAGC 30 0.00475105 29.999998 13 CTCGTAT 30 0.00475105 29.999998 39 >>END_MODULE