##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_81.34100000006720.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 100192 Sequences flagged as poor quality 0 Sequence length 51 %GC 24 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.40154902587033 38.0 38.0 38.0 32.0 38.0 2 36.28006228042159 38.0 38.0 38.0 32.0 38.0 3 36.16147995847972 38.0 38.0 38.0 32.0 38.0 4 36.13463150750559 38.0 38.0 38.0 32.0 38.0 5 36.15603042159054 38.0 38.0 38.0 32.0 38.0 6 38.10204407537528 40.0 38.0 40.0 38.0 40.0 7 37.98635619610348 40.0 38.0 40.0 32.0 40.0 8 37.92667079207921 40.0 38.0 40.0 32.0 40.0 9 37.83245169274992 40.0 38.0 40.0 32.0 40.0 10 37.72818189076972 40.0 38.0 40.0 32.0 40.0 11 37.709777227722775 40.0 38.0 40.0 32.0 40.0 12 37.7026808527627 40.0 38.0 40.0 32.0 40.0 13 37.702531140210795 40.0 38.0 40.0 32.0 40.0 14 37.68357753114021 40.0 38.0 40.0 32.0 40.0 15 37.86676580964548 40.0 38.0 40.0 32.0 40.0 16 37.89615937400192 40.0 38.0 40.0 32.0 40.0 17 38.027996247205365 40.0 38.0 40.0 32.0 40.0 18 38.02003153944427 40.0 38.0 40.0 32.0 40.0 19 37.993811881188115 40.0 38.0 40.0 32.0 40.0 20 37.95950774512935 40.0 38.0 40.0 32.0 40.0 21 37.98581723091664 40.0 38.0 40.0 32.0 40.0 22 37.95563518045353 40.0 38.0 40.0 32.0 40.0 23 37.99238462152667 40.0 38.0 40.0 32.0 40.0 24 38.02331523474928 40.0 38.0 40.0 32.0 40.0 25 38.045063478122 40.0 38.0 40.0 32.0 40.0 26 38.000548946023635 40.0 38.0 40.0 32.0 40.0 27 37.90137935164484 40.0 38.0 40.0 32.0 40.0 28 37.81729080166081 40.0 38.0 40.0 32.0 40.0 29 37.69363821462792 40.0 38.0 40.0 32.0 40.0 30 37.52560084637496 40.0 38.0 40.0 32.0 40.0 31 37.29542278824657 40.0 38.0 40.0 32.0 40.0 32 36.969688198658574 40.0 38.0 40.0 32.0 40.0 33 36.565494251038004 40.0 38.0 40.0 27.0 40.0 34 36.21233232194187 40.0 38.0 40.0 27.0 40.0 35 35.773454966464385 40.0 38.0 40.0 13.0 40.0 36 35.32819985627595 40.0 38.0 40.0 13.0 40.0 37 35.02324536889173 40.0 38.0 40.0 13.0 40.0 38 34.66732872884062 40.0 38.0 40.0 13.0 40.0 39 34.23337192590227 40.0 32.0 40.0 13.0 40.0 40 33.79715945384861 38.0 32.0 40.0 13.0 40.0 41 33.26425263494092 38.0 27.0 40.0 13.0 40.0 42 32.63634821143405 38.0 27.0 40.0 13.0 40.0 43 31.953838629830724 38.0 27.0 40.0 13.0 40.0 44 31.269462631747047 38.0 27.0 40.0 13.0 40.0 45 30.27310563717662 38.0 13.0 40.0 13.0 40.0 46 29.237494011497922 38.0 13.0 40.0 13.0 40.0 47 28.079317709996808 32.0 13.0 40.0 13.0 40.0 48 26.97702411370169 27.0 13.0 40.0 13.0 40.0 49 25.966065154902587 27.0 13.0 38.0 13.0 40.0 50 25.036320265091025 27.0 13.0 38.0 13.0 40.0 51 24.13951213669754 27.0 13.0 38.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.27692739388336207 2201 2 0.17785794179098247 2201 3 0.20626584792812253 2201 4 0.3548837255157977 2201 5 0.6553102973316385 2201 6 0.5261571450030544 2201 7 0.36705863805354966 2201 8 0.15558342077019915 2201 9 0.6533960804719996 2201 10 0.0012768611738067648 2201 11 -0.183808127225511 2201 12 0.059946814876880694 2201 13 0.00607359845885469 2201 14 0.415314329935093 2201 15 -0.09312690368461318 2201 16 0.12716339691343137 2201 17 -0.03299872553041894 2201 18 0.2996165322397033 2201 19 -0.08918305571650365 2201 20 0.1201597336552993 2201 21 0.29564178114983974 2201 22 -0.30818537226536336 2201 23 0.23440585567962557 2201 24 0.3027411323093361 2201 25 0.014693792711369724 2201 26 -0.25251970632507437 2201 27 0.6244656771040269 2201 28 -0.47133206878152833 2201 29 -0.7450384537156367 2201 30 0.09669546791255357 2201 31 0.13938830671798286 2201 32 0.4382989183462911 2201 33 -0.4238984596447608 2201 34 0.5488934245115544 2201 35 0.6002274008789357 2201 36 0.6130980111573265 2201 37 0.633658543022861 2201 38 0.8301547411017225 2201 39 0.39476587243841976 2201 40 1.2683943496319117 2201 41 1.6734812387579225 2201 42 1.3071855462785322 2201 43 1.513679446292592 2201 44 1.6734497601216134 2201 45 2.01148462163982 2201 46 1.4823688788501812 2201 47 1.7299749625417995 2201 48 1.0356372899716213 2201 49 0.9553890682110726 2201 50 2.0094418069098943 2201 51 2.000523771424426 1101 1 0.4338946584011083 1101 2 -0.193896730227074 1101 3 0.2795757689114424 1101 4 -0.24081425516901334 1101 5 0.42321447392765066 1101 6 0.08617219579949165 1101 7 0.6354017962123351 1101 8 -0.24470505282820199 1101 9 0.3740156715920264 1101 10 0.2814098098756759 1101 11 -0.02665272775229255 1101 12 0.11519578013462706 1101 13 0.06326662492317325 1101 14 0.5728459993192629 1101 15 0.3818511472372492 1101 16 0.20730888794566482 1101 17 -0.7102845652394336 1101 18 0.23677945711114035 1101 19 0.3791475601119174 1101 20 0.09024627573482036 1101 21 0.4763140500574039 1101 22 0.11844199509949505 1101 23 0.09336735072540137 1101 24 0.2837395018063873 1101 25 -0.10107191655740877 1101 26 0.18426694863541826 1101 27 0.012023685381969074 1101 28 0.2168028866932019 1101 29 0.058235094509626606 1101 30 0.37977586425019183 1101 31 0.029893762631687082 1101 32 -0.14134487947134744 1101 33 0.11241158676185847 1101 34 -1.974184463360011 1101 35 -0.3776980976786959 1101 36 -0.8765545662915599 1101 37 0.09095190813009424 1101 38 0.21765003772783587 1101 39 -0.16568815992079067 1101 40 0.41840062079709384 1101 41 -0.009385937963514834 1101 42 -0.45695593120798605 1101 43 -0.9412308760452994 1101 44 -0.057140983638579 1101 45 0.09683517977352452 1101 46 0.9125708103690577 1101 47 0.08354325553063546 1101 48 0.2528077402412805 1101 49 0.08513947583680803 1101 50 -0.7153794648824032 1101 51 -0.7765990179898452 2202 1 0.4134820157321002 2202 2 -0.09315046157036022 2202 3 0.12538349498694146 2202 4 0.06076607845697879 2202 5 -0.3940594505675179 2202 6 0.09023277525515283 2202 7 -0.2926052274926647 2202 8 -0.4389543943558536 2202 9 0.05465658467367973 2202 10 0.11997434016539898 2202 11 0.24266246100977895 2202 12 0.0956611005911725 2202 13 0.13317443879498825 2202 14 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2202 50 -1.829843907375821 2202 51 -1.5236358924411206 1102 1 0.2079039224258139 1102 2 -0.17073666846691538 1102 3 0.04277700411473262 1102 4 -0.10231708584322519 1102 5 -0.013481357261227345 1102 6 0.16573987464663276 1102 7 -0.6863171950383062 1102 8 -0.6777982077432796 1102 9 -0.4148160319591838 1102 10 -0.10999523387111054 1102 11 -0.24425437469001565 1102 12 0.121320329800092 1102 13 -0.2830020317932451 1102 14 -0.15211540057780581 1102 15 -0.12355286717345138 1102 16 0.06381943864046491 1102 17 -0.14548785911488693 1102 18 -0.1444866262650777 1102 19 0.05778883648865474 1102 20 0.5081052566920974 1102 21 -0.24437560511501033 1102 22 0.25014554630896413 1102 23 0.08940440682421524 1102 24 0.45713116418415467 1102 25 0.01730173243898747 1102 26 0.21036061821853735 1102 27 0.1900988269156798 1102 28 0.48520226909155184 1102 29 0.9504121403150805 1102 30 0.8623286177242022 1102 31 0.312961184144811 1102 32 0.3222166233593313 1102 33 1.1694368054957778 1102 34 0.8783631434336101 1102 35 1.0365067402008705 1102 36 1.7400177445679361 1102 37 1.514214903498086 1102 38 1.6389058277432795 1102 39 1.4432155971558984 1102 40 1.0958066938799504 1102 41 0.45366089682804756 1102 42 0.9130389221116246 1102 43 1.5049863138672066 1102 44 0.46637934574896533 1102 45 1.0176277685537585 1102 46 0.6588394670854747 1102 47 0.8862195293340349 1102 48 2.1251185997369078 1102 49 0.3512743188630587 1102 50 1.2355212041860462 1102 51 1.4973073124270329 2203 1 0.01480047414995056 2203 2 0.587884021188259 2203 3 0.20139769376990557 2203 4 0.9390622245015976 2203 5 0.37962209101463174 2203 6 0.17263642637077226 2203 7 0.2723034946168639 2203 8 -0.16055682843293084 2203 9 0.1920805197649571 2203 10 0.24172890405992575 2203 11 0.5419525218617096 2203 12 0.16959619186906139 2203 13 0.3307910716550353 2203 14 0.26167770287047176 2203 15 0.48334368455068244 2203 16 -0.053421940669878154 2203 17 0.0889948995057992 2203 18 0.11068578752801983 2203 19 0.4991681468334832 2203 20 0.025346636002446132 2203 21 0.11424508454016546 2203 22 0.16049037389517196 2203 23 0.39285268268628926 2203 24 0.2847173710807098 2203 25 0.6793706979562302 2203 26 0.21725716994266975 2203 27 0.6452712407087802 2203 28 0.16796088978120594 2203 29 0.7917914506599075 2203 30 0.44163896255179225 2203 31 0.45089221862757256 2203 32 0.5842855888765683 2203 33 1.1901264606681892 2203 34 1.036983833088783 2203 35 1.4571963953732876 2203 36 1.5055349859472429 2203 37 1.7486976621187722 2203 38 1.5561472070536198 2203 39 1.4776983557765888 2203 40 0.7509791076730536 2203 41 0.5846953795866696 2203 42 0.2578665083185143 2203 43 0.33946907248789415 2203 44 0.36982762161103366 2203 45 0.010731216829622525 2203 46 -0.21012605015590324 2203 47 -0.562056332734933 2203 48 -0.51626071060792 2203 49 -0.8418291294128046 2203 50 -0.3782718992622307 2203 51 0.5386866227718627 1103 1 -0.08610948753587166 1103 2 0.11518287176297548 1103 3 -0.01105441350978964 1103 4 -0.14307379082406868 1103 5 0.3069209415893468 1103 6 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-1.22457393874231 1213 28 -0.9182108202821269 1213 29 -1.3143661848080725 1213 30 -1.4105074132399054 1213 31 -1.0703956068474483 1213 32 -1.3480156074640348 1213 33 -1.127079732153767 1213 34 -1.129377884011852 1213 35 -1.1236621549433892 1213 36 -1.1873221569099002 1213 37 -1.0152010007946686 1213 38 -1.2850913573376488 1213 39 -1.3150180411270043 1213 40 -1.0014135735373557 1213 41 -1.0387036351916592 1213 42 -0.9392130131452419 1213 43 -0.7267167121707949 1213 44 -1.1524806120272473 1213 45 -0.6457782835222439 1213 46 -0.27993604311860665 1213 47 0.1582462710652095 1213 48 -1.1474359393200935 1213 49 -0.7633984467949304 1213 50 -0.43773706463872486 1213 51 -0.47816770516621077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 4.0 15 6.0 16 24.0 17 22.0 18 14.0 19 22.0 20 37.0 21 84.0 22 133.0 23 284.0 24 485.0 25 751.0 26 1094.0 27 1600.0 28 2200.0 29 2977.0 30 4033.0 31 5192.0 32 5965.0 33 6932.0 34 7915.0 35 8757.0 36 10118.0 37 11608.0 38 10939.0 39 18996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 67.28603378729183 12.239905747034625 14.088022684612007 6.386037781061544 2 11.2825631300529 29.21748677512726 48.43696975746082 11.062980337359019 3 25.712631747045673 43.86378153944427 23.075694666240818 7.347892047269243 4 9.999800383264134 15.369490578090067 36.514891408495686 38.11581763015011 5 17.7988262535931 38.88933248163526 22.125519003513254 21.186322261258383 6 23.81327850526988 39.03006228042159 11.487943149153624 25.6687160651549 7 26.541041200894284 35.897077610986905 23.04974449057809 14.512136697540722 8 33.570544554455445 17.791839667837753 17.986465985308207 30.651149792398595 9 31.936681571382948 16.708918875758545 26.713709677419356 24.640689875439158 10 28.471335036729478 27.791640051101886 20.30102203768764 23.436002874480995 11 23.10057092665788 31.161416536910608 27.88058450113786 17.857428035293648 12 37.339308527626955 28.72983870967742 29.031259980836793 4.899592781858831 13 28.762775471095498 30.507425742574256 31.265969338869372 9.463829447460876 14 22.9828728840626 23.692510380070264 46.73027786649633 6.594338869370809 15 11.690554136058767 30.824816352603 43.44857872884062 14.036050782497606 16 6.254054755417153 38.636204848738906 39.670229860965556 15.439510534878382 17 9.634597917976665 27.41264185405875 34.42524777674642 28.527512451218172 18 16.804072262700867 22.67691386365905 45.76404830821439 14.75496556542569 19 21.455804854679016 22.118532417757905 49.32729160012775 7.098371127435324 20 15.557130309805173 18.5234350047908 50.05389651868413 15.865538166719897 21 17.80581283934845 24.05182050463111 52.29958479718939 5.842781858831044 22 10.407018524433088 25.684685404024272 53.86158575534973 10.04671031619291 23 11.49592781858831 31.55441552219738 53.03417438518046 3.9154822740338555 24 9.67043276908336 33.159334078569145 53.782737144682216 3.3874960076652827 25 4.864659853082083 36.43005429575216 55.2149872245289 3.490298626636857 26 3.6889540976734434 38.40065474942859 53.94097274206266 3.969418410835304 27 2.5880309805174067 37.82337911210476 53.88354359629511 5.705046311082721 28 2.328529223890131 39.09693388693708 51.060962951133824 7.513573938038966 29 2.8984350047908016 41.28074097732354 49.431092302778666 6.389731715106994 30 3.976524134626901 40.0726633928215 48.75933245498463 7.191480017566974 31 5.517406579367614 37.57086394123284 48.14855477483232 8.76317470456723 32 6.724089747684446 38.11382146279144 47.676461194506544 7.485627595017566 33 5.0433168316831685 40.12795432769084 47.39100926221654 7.437719578409453 34 4.600167678058129 43.91667997444906 46.45879910571703 5.02435324177579 35 4.52131906739061 46.001676780581285 45.661330245927815 3.815673906100287 36 4.465426381347813 46.65941392526349 45.210196422868094 3.664963270520601 37 4.267805812839348 47.72037687639732 44.66923506866816 3.3425822420951774 38 4.257824976045992 47.758304056212076 44.26501117853721 3.7188597892047266 39 4.0272676461194505 48.20045512615778 43.48950015969339 4.282777068029383 40 4.227882465665921 48.423027786649634 42.68903704886618 4.660052698818269 41 4.320704247844139 48.503872564675824 41.362583839029064 5.812839348450974 42 4.677066802407402 48.38957591001188 39.98063698336178 6.952720304218942 43 4.312719578409454 47.82317949536889 39.07297987863302 8.791121047588629 44 4.478401469179176 47.01972213350367 37.945145320983706 10.55673107633344 45 4.9574816352603 45.56251996167359 36.64763653784733 12.83236186521878 46 5.888693708080486 44.34485787288406 34.87903225806452 14.887416160970938 47 6.624347496282102 43.296304059246836 33.102773702228745 16.976574742242317 48 7.272037687639732 42.14607952730757 31.876796550622803 18.705086234429892 49 8.303058128393484 40.93240977323539 31.240019163206647 19.524512935164484 50 8.261138613861387 39.753672947939954 31.416679974449057 20.568508463749602 51 8.70164091507965 38.70124166566855 30.78312771988661 21.813989699365194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 27.0 1 18.0 2 9.0 3 34.5 4 60.0 5 92.0 6 124.0 7 147.5 8 171.0 9 281.0 10 391.0 11 923.5 12 1456.0 13 3446.0 14 5436.0 15 7683.0 16 9930.0 17 10700.0 18 11470.0 19 10785.0 20 10100.0 21 9410.5 22 8721.0 23 9751.5 24 10782.0 25 10929.5 26 8176.5 27 5276.0 28 3988.0 29 2700.0 30 2544.0 31 2388.0 32 3879.0 33 5370.0 34 5160.5 35 4951.0 36 3817.5 37 2684.0 38 1867.5 39 1051.0 40 835.5 41 620.0 42 622.0 43 624.0 44 659.0 45 694.0 46 778.0 47 862.0 48 815.0 49 768.0 50 619.5 51 471.0 52 451.0 53 431.0 54 401.0 55 371.0 56 312.5 57 254.0 58 247.0 59 240.0 60 210.0 61 180.0 62 162.5 63 145.0 64 120.5 65 96.0 66 90.0 67 84.0 68 68.0 69 52.0 70 44.0 71 36.0 72 34.0 73 32.0 74 27.5 75 17.0 76 11.0 77 11.0 78 11.0 79 10.0 80 9.0 81 6.0 82 3.0 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03593101245608432 2 0.001996167358671351 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.003992334717342702 12 0.0 13 0.0 14 0.0 15 0.0 16 9.980836793356755E-4 17 9.980836793356755E-4 18 0.001996167358671351 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 9.980836793356755E-4 27 0.0 28 0.0 29 0.0 30 0.003992334717342702 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 9.980836793356755E-4 43 0.0 44 0.0 45 0.0 46 0.0 47 9.980836793356755E-4 48 0.0 49 0.0 50 0.0 51 0.003992334717342702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 100192.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.5466184141877 #Duplication Level Percentage of deduplicated Percentage of total 1 87.29544705708241 33.6494428700528 2 5.648716234760643 4.3547781846269125 3 2.1585476442921294 2.496141371201175 4 1.1503499274289393 1.7736839878156736 5 0.7921229937161994 1.526683138794117 6 0.43090793629142904 0.9966026275122492 7 0.3725379186716996 1.0052053897097588 8 0.24140640363047414 0.7444320418788212 9 0.239666245147832 0.8314490968627181 >10 1.3027691010349518 9.655489900881213 >50 0.17064016376164182 4.414059602556412 >100 0.149635164390843 11.635027076443084 >500 0.023626604895432415 6.374068145818724 >1k 0.023626604895432415 20.542936565846308 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3318 3.3116416480357715 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3198 3.19187160651549 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 2959 2.953329607154264 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2699 2.693827850526988 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 1905 1.9013494091344618 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1833 1.8294873842222934 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1818 1.8145161290322582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1475 1.4721734270201214 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 1096 1.0938997125519003 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 993 0.9910970935803257 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 895 0.8932848930054296 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 777 0.7755110188438199 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 744 0.7425742574257426 No Hit GTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 638 0.636777387416161 No Hit GTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 611 0.6098291280740977 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 610 0.608831044394762 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 529 0.5279862663685724 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 502 0.501038007026509 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.4581204088150751 No Hit TATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 458 0.4571223251357394 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 441 0.4401549025870329 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 424 0.4231874800383264 No Hit TATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 397 0.39623922069626316 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 319 0.3183886937080805 No Hit GAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.3094059405940594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 268 0.26748642606196105 No Hit GGTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 265 0.264492175023954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 259 0.25850367294793997 No Hit TCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 259 0.25850367294793997 No Hit GCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 256 0.2555094219099329 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 255 0.25451133823059724 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 255 0.25451133823059724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT 245 0.24453050143724048 No Hit ATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 242 0.2415362503992335 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 241 0.24053816671989778 No Hit ATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 240 0.23954008304056212 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.23554774832321942 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 231 0.23055732992654107 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT 209 0.2085994889811562 No Hit GTATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 208 0.2076014053018205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 204 0.2036090705844778 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 197 0.19662248482912809 No Hit GGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 192 0.19163206643244968 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 185 0.18464548067709996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 177 0.17666081124241456 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 174 0.17366656020440752 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 173 0.17266847652507186 No Hit GTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172 0.1716703928457362 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 167 0.16667997444905783 No Hit GTATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAA 164 0.16368572341105078 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 160 0.15969338869370808 No Hit TATCAACGCAGAGTACGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 160 0.15969338869370808 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 160 0.15969338869370808 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT 155 0.1547029702970297 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 152 0.15170871925902268 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGT 152 0.15170871925902268 No Hit GTATCAACGCAGAGTACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 146 0.14572021718300862 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA 144 0.14372404982433729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 138 0.13773554774832322 No Hit CAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA 128 0.12775471095496646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTT 125 0.12476045991695944 No Hit GTGGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAA 124 0.12376237623762376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT 124 0.12376237623762376 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 124 0.12376237623762376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 122 0.12176620887895241 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC 120 0.11977004152028106 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT 117 0.11677579048227403 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 116 0.11577770680293836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 109 0.10879112104758863 No Hit TATCAACGCAGAGTACGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 108 0.10779303736825295 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 107 0.10679495368891728 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT 107 0.10679495368891728 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTT 107 0.10679495368891728 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.10679495368891728 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 104 0.10380070265091025 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 9.980836793356755E-4 20 0.0 0.0 0.003992334717342702 21 0.0 0.0 0.011977004152028107 22 0.0 0.0 0.023954008304056213 23 0.0 0.0 0.06387735547748323 24 0.0 0.0 0.11278345576493133 25 0.0 0.0 0.12775471095496646 26 0.0 0.0 0.18863781539444266 27 0.0 0.0 0.23454966464388374 28 0.0 0.0 0.30641168955605236 29 0.0 0.0 0.4291759821143405 30 0.0 0.0 0.5778904503353561 31 0.0 0.0 0.6747045672309167 32 0.0 0.0 0.796470776109869 33 0.0 0.0 0.9052618971574576 34 0.0 0.0 1.021039603960396 35 0.0 0.0 1.1398115618013414 36 0.0 0.0 1.2266448419035452 37 0.0 0.0 1.3234589587991057 38 0.0 0.0 1.4621925902267645 39 0.0 0.0 1.6937480038326413 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 35 1.1979682E-7 44.996006 45 AATTACT 25 0.0020988707 35.996803 43 GTATGCC 45 8.6009277E-7 34.99689 42 ATCTCGT 45 8.6009277E-7 34.99689 37 CCACGAG 45 8.6009277E-7 34.99689 20 TATGCCG 45 8.6009277E-7 34.99689 43 CACGAGA 45 8.6009277E-7 34.99689 21 CCCACGA 45 8.6009277E-7 34.99689 19 ACGAGAC 45 8.6009277E-7 34.99689 22 TATCTCG 45 8.6009277E-7 34.99689 36 ATGCCGT 45 8.6009277E-7 34.99689 44 CGTATGC 45 8.6009277E-7 34.99689 41 TCGTATG 45 8.6009277E-7 34.99689 40 CTCGTAT 45 8.6009277E-7 34.99689 39 GAGACAA 45 8.6009277E-7 34.99689 24 CAACATA 40 1.5473819E-5 33.747005 28 ATAATAT 40 1.5473819E-5 33.747005 32 ACATAAT 40 1.5473819E-5 33.747005 30 GACAACA 40 1.5473819E-5 33.747005 26 TAATATC 40 1.5473819E-5 33.747005 33 >>END_MODULE