FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_80.34100000006713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_80.34100000006713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299606
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC29080.9706080652590402No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG3250.040.1522136
CGAGACT3250.040.1522123
CGAGCCC3400.039.04903815
TCGTATG3400.039.04252640
ACGAGAC3350.038.95363622
CACGAGA3400.038.38078721
GCCCACG3500.037.93335318
TATGCCG3500.037.9270243
TGCCGTC3500.037.9270245
CTCGTAT3500.037.2841939
TCTCGTA3550.036.75906438
CGTATGC3700.036.48541
CCGAGCC3750.036.0045414
ATAGTAC250.00210504935.998533
CCCACGA3700.035.87691519
TCTCCGA3850.035.64789611
GACTTGC3700.035.26883326
ATGCCGT3900.034.6139744
ATCTCCG3850.034.47911510
CCACGAG3850.034.47911520
Produced by FastQC (version 0.11.2)