FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_78.341000000066f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_78.341000000066f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470876
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG801.051103E-725.3123243
CGAGCCC901.1872544E-824.99982315
GTATTAA551.3636405E-424.5531021
ACGCCTA651.5213538E-524.23059821
ACGAGAC851.8888386E-723.8233622
GGTATCA5550.022.3042451
GTATCAA10450.022.1839431
AATCTCG955.5178134E-721.3156436
CGCCTAA754.5954766E-520.99985122
ACGGGGT2150.020.93008416
CTCTCGC550.00393330120.45440120
CTGTGCG550.00393330120.4544019
TCGTATG1009.0322465E-720.24985740
CGGGGTG1800.019.99985917
GCCTAAT807.551309E-519.6873623
TGTAGCA807.551309E-519.687362
CTCGTAT1051.4419657E-619.28557839
CGAGACT706.9754256E-419.28557823
ACGGGTA1302.7621354E-819.03832616
GGTAAAC600.006505186718.7498663
Produced by FastQC (version 0.11.2)