FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_77.341000000066eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_77.341000000066eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551982
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCTA406.159728E-428.12681230
TATGCCG654.866779E-727.69158443
GGCGCCA450.001225286325.00161224
TAAACAG1001.3424142E-924.7493535
TCGTATG802.9707826E-622.4994140
GTATCAA11950.021.4755481
CTCGTAT855.0255294E-621.17591539
TTTGCGT855.0255294E-621.1759153
TTTTGCG855.0255294E-621.1759152
TAAGGTG654.235133E-420.7686885
GGTATCA6400.020.0494371
AAACGAG706.9741503E-419.28695727
GCCCACG951.3141051E-518.94687318
TCTCGTA951.3141051E-518.94687338
TGCCGTC951.3141051E-518.94687345
TCTCCGA5900.017.9232645
CTCCGAG4000.017.43704445
CGTATGC1053.108385E-517.14240841
TTGCGTT1053.108385E-517.1424084
TAGAACA800.001709623316.8745574
Produced by FastQC (version 0.11.2)