FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_76.341000000066de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_76.341000000066de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430558
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC950.035.52567322
TATGCCG1000.033.7493943
CGAGACT1000.031.49943223
TCTCGTA1100.030.68126338
TATAAAT300.005141259630.00294310
CACGACA300.005144180729.9994584
ATGCCGT1200.028.12449344
CGAGCCC1250.026.99951215
TGCCGTC1250.026.99951245
GCCCACG1300.025.9610718
GGTATCA3650.025.2735141
CCACGAG1350.024.9995520
CCCACGA1450.024.8271419
GTATTAA551.3664302E-424.545011
AATCTCG1101.5097612E-1024.5450136
CTCGTAT1400.024.10670939
CGTATGC1450.023.27544441
TCGTATG1550.023.22538640
TGCGCTT500.002261038222.50220710
ATCGTAG500.002262564822.49959433
Produced by FastQC (version 0.11.2)