##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_75.341000000066c1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6889 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.57076498766149 38.0 32.0 38.0 32.0 38.0 2 35.5970387574394 38.0 32.0 38.0 32.0 38.0 3 35.63724778632603 38.0 32.0 38.0 32.0 38.0 4 35.84293801712876 38.0 32.0 38.0 32.0 38.0 5 35.94411380461605 38.0 32.0 38.0 32.0 38.0 6 37.69081143852519 40.0 38.0 40.0 32.0 40.0 7 37.52852373348817 40.0 38.0 40.0 32.0 40.0 8 37.493975903614455 38.0 38.0 40.0 32.0 40.0 9 37.47452460444186 38.0 38.0 40.0 32.0 40.0 10 37.417186819567426 40.0 38.0 40.0 32.0 40.0 11 37.499637102627375 38.0 38.0 40.0 32.0 40.0 12 37.384235738133256 38.0 38.0 40.0 32.0 40.0 13 37.41457395848454 38.0 38.0 40.0 32.0 40.0 14 37.19306140223545 38.0 38.0 40.0 32.0 40.0 15 37.283640586442154 38.0 38.0 40.0 32.0 40.0 16 37.18841631586587 38.0 38.0 40.0 32.0 40.0 17 37.125997967774715 38.0 38.0 40.0 32.0 40.0 18 37.128901146755695 38.0 38.0 40.0 32.0 40.0 19 37.20931920452896 38.0 38.0 40.0 32.0 40.0 20 37.1654812019161 38.0 38.0 40.0 32.0 40.0 21 37.16373929452751 38.0 38.0 40.0 32.0 40.0 22 37.284656699085495 38.0 38.0 40.0 32.0 40.0 23 37.30614022354478 38.0 38.0 40.0 32.0 40.0 24 37.29902743504137 38.0 38.0 40.0 32.0 40.0 25 37.207141820293224 38.0 38.0 40.0 32.0 40.0 26 37.100014515894905 38.0 38.0 40.0 32.0 40.0 27 37.023515749745975 38.0 38.0 40.0 32.0 40.0 28 37.05690230802729 38.0 38.0 40.0 32.0 40.0 29 37.017709391784 38.0 38.0 40.0 32.0 40.0 30 37.08012773987517 38.0 38.0 40.0 32.0 40.0 31 36.953258818406155 38.0 38.0 40.0 32.0 40.0 32 36.93119465815067 38.0 38.0 40.0 32.0 40.0 33 36.88430831760778 38.0 38.0 40.0 32.0 40.0 34 36.93046886340543 38.0 38.0 40.0 32.0 40.0 35 36.9356945855712 38.0 38.0 40.0 32.0 40.0 36 36.81927710843374 38.0 38.0 40.0 32.0 40.0 37 36.749600812890115 38.0 38.0 40.0 32.0 40.0 38 36.831760778051965 38.0 38.0 40.0 32.0 40.0 39 36.85687327623748 38.0 38.0 40.0 32.0 40.0 40 36.829438234867176 38.0 38.0 40.0 32.0 40.0 41 36.87850195964581 38.0 38.0 40.0 32.0 40.0 42 36.796777471331104 38.0 38.0 40.0 32.0 40.0 43 36.95137175206852 38.0 38.0 40.0 32.0 40.0 44 36.911307882130934 38.0 38.0 40.0 32.0 40.0 45 36.98432283350269 38.0 38.0 40.0 32.0 40.0 46 36.67861808680505 38.0 38.0 40.0 32.0 40.0 47 36.92974306866018 38.0 38.0 40.0 32.0 40.0 48 36.789519523878646 38.0 38.0 40.0 32.0 40.0 49 36.782842212222384 38.0 38.0 40.0 32.0 40.0 50 36.56365219915808 38.0 38.0 40.0 32.0 40.0 51 35.98403251560459 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 -2.4157382548007647 2201 2 -0.9252306721056769 2201 3 1.0798320603008094 2201 4 -0.46031117320179504 2201 5 -0.19868022602398128 2201 6 -0.06381114545176558 2201 7 0.8505935037185068 2201 8 1.9055732895576512 2201 9 0.9673510517260482 2201 10 -0.27991613421300343 2201 11 0.08116978507602823 2201 12 0.042973216020094185 2201 13 -2.949581061299824 2201 14 -4.743844566891447 2201 15 -1.4441193355255777 2201 16 0.5290355911449751 2201 17 0.909680900696543 2201 18 0.7016006996475852 2201 19 0.12880153700466934 2201 20 -2.081088486166621 2201 21 1.0494965971528458 2201 22 0.6553025099900154 2201 23 0.03877654463592961 2201 24 -0.5461221937784444 2201 25 -1.5359822469197368 2201 26 -3.2566094062187716 2201 27 -0.5788212221806077 2201 28 -3.230928359834614 2201 29 -2.0471280455655574 2201 30 -2.252736196095576 2201 31 0.7072690330502809 2201 32 1.7926270344239157 2201 33 1.3663536550255344 2201 34 -1.2418388035575703 2201 35 -0.09209752920689596 2201 36 0.009472211815975129 2201 37 1.4956805781024443 2201 38 -0.5096331619769146 2201 39 -2.0261263042513207 2201 40 1.7866485511016634 2201 41 -2.1112501647657993 2201 42 -1.6917172237484976 2201 43 0.05839191190752757 2201 44 1.5552442782911413 2201 45 -2.021236619283499 2201 46 -5.075879675879669 2201 47 -0.6565946640165308 2201 48 0.31430869477743073 2201 49 0.9776130553474403 2201 50 -0.1655708874458881 2201 51 -1.6208819392413218 1101 1 1.612039522977014 1101 2 1.019213772338766 1101 3 0.8020542825230308 1101 4 0.5952443823537621 1101 5 2.356875329531576 1101 6 1.9917444101037916 1101 7 1.6283712814962854 1101 8 -1.8027600437756846 1101 9 2.217351051726048 1101 10 -1.2660272453241177 1101 11 0.6506142295204711 1101 12 0.08463988268675848 1101 13 0.7031967164779545 1101 14 1.0617109886641103 1101 15 0.37532510891886517 1101 16 1.2512578133671965 1101 17 1.0069031229187644 1101 18 -1.7011770781301934 1101 19 1.0454682036713336 1101 20 0.32168929161115756 1101 21 1.8272743749306244 1101 22 0.6553025099900154 1101 23 1.705443211302594 1101 24 1.2872111395548913 1101 25 2.2695733086358203 1101 26 2.1045017048923427 1101 27 2.2267343333749494 1101 28 -1.6337061376123927 1101 29 -1.8110169344544431 1101 30 0.5805971372377599 1101 31 0.5406023663836166 1101 32 1.15373814553503 1101 33 -0.730868567196687 1101 34 0.6887167519979869 1101 35 1.157902470793104 1101 36 -0.671083343739582 1101 37 -1.3793194218975557 1101 38 1.1709223935786426 1101 39 0.682207029082015 1101 40 1.8699818844349991 1101 41 2.110972057456422 1101 42 0.16939388736261662 1101 43 1.280614134129749 1101 44 1.6107998338466984 1101 45 -3.7990143970612777 1101 46 1.104675879675888 1101 47 -2.378816886238752 1101 48 1.300419805888545 1101 49 0.8248352775696617 1101 50 -1.485015331890331 1101 51 2.9346736163142353 2202 1 0.3620395229770139 2202 2 1.519213772338766 2202 3 1.3020542825230308 2202 4 0.5952443823537621 2202 5 1.8568753295315759 2202 6 0.9917444101037916 2202 7 1.7950379481629497 2202 8 1.5722399562243154 2202 9 1.9673510517260482 2202 10 -0.307693911990782 2202 11 0.7756142295204711 2202 12 1.7096398826867585 2202 13 0.07819671647795445 2202 14 1.4783776553307746 2202 15 1.6669917755855295 2202 16 1.5845911467005322 2202 17 2.2985697895854287 2202 18 1.9238229218698066 2202 19 1.962134870337998 2202 20 1.1966892916111576 2202 21 2.3272743749306244 2202 22 0.6553025099900154 2202 23 1.3721098779692582 2202 24 1.120544472888227 2202 25 -0.397093358030844 2202 26 1.8545017048923427 2202 27 1.9767343333749494 2202 28 1.9912938623876073 2202 29 2.230649732212221 2202 30 2.247263803904424 2202 31 2.3739356997169523 2202 32 1.5704048122016943 2202 33 2.3107980994699773 2202 34 2.980383418664651 2202 35 2.5745691374597683 2202 36 2.6205833229270823 2202 37 2.32901391143578 2202 38 2.212589060245307 2202 39 1.9738736957486793 2202 40 2.453315217768335 2202 41 1.9443053907897578 2202 42 2.086060554029281 2202 43 -0.3027191992035867 2202 44 -0.47253349948663725 2202 45 1.1176522696053866 2202 46 1.729675879675888 2202 47 1.787849780427912 2202 48 -0.4912468607781193 2202 49 2.366501944236326 2202 50 1.389984668109669 2202 51 1.1846736163142353 1102 1 0.8620395229770139 1102 2 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0.48012816176878204 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 2.0 22 5.0 23 7.0 24 23.0 25 26.0 26 52.0 27 59.0 28 92.0 29 124.0 30 166.0 31 200.0 32 221.0 33 254.0 34 305.0 35 368.0 36 515.0 37 711.0 38 1151.0 39 2606.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.832704037176878 15.945396456578564 9.802497821667151 42.4194016845774 2 15.183626070547248 15.575555232980113 48.97662940920308 20.26418928726956 3 15.952968500508055 19.93032370445638 41.38481637392945 22.731891421106113 4 13.122368994048482 29.481782551894327 34.06880534184932 23.327043112207868 5 10.886921178690665 26.897953258818408 47.8153578168094 14.39976774568152 6 23.18188416315866 50.87821164174772 15.256205545071854 10.683698650021773 7 24.256060386122805 22.122223835099433 24.546378284221223 29.075337494556543 8 18.362607054724926 48.0185803454783 17.0271447234722 16.591667876324575 9 17.50616925533459 15.69168239221948 17.361010306285383 49.441138046160546 10 14.240092901727392 43.358978080998696 24.096385542168676 18.30454347510524 11 23.41413848163739 20.58353897517782 34.01074176222964 21.991580780955147 12 17.361010306285383 25.214109449847584 24.21251270140804 33.21236754245899 13 22.006096675860068 16.765858615183625 40.62998983887356 20.59805487008274 14 16.794890404993467 18.406154739439685 19.14646537959065 45.652489475976196 15 18.406154739439685 21.469008564377994 26.157642618667442 33.967194077514876 16 33.923646392800116 21.04804761213529 21.41094498475831 23.617361010306283 17 15.909420815793293 36.23167368268253 23.67542458992597 24.183480911598203 18 36.913920743213815 20.830309188561476 22.20931920452896 20.046450863695746 19 18.81259979677747 26.273769777906807 21.323849615328786 33.589780809986934 20 21.73029467266657 22.325446363768325 20.75772971403687 35.186529249528235 21 17.752939468718246 23.123820583538976 19.219044854115257 39.90419509362752 22 20.365800551604007 34.736536507475684 19.97387138917114 24.923791551749165 23 15.285237334881696 22.688343736391346 22.35447815357817 39.67194077514879 24 34.2575119756133 26.651183045434752 21.164174771374654 17.927130207577296 25 18.44970242415445 37.363913485266366 22.238350994338802 21.948033096240383 26 39.198606271777 22.34320557491289 21.036585365853657 17.421602787456447 27 17.927130207577296 42.255770068224706 21.323849615328786 18.493250108869212 28 21.802874147191172 19.683553491072725 21.77384235738133 36.73973000435477 29 16.635215561039338 26.128610828857603 37.42197706488605 19.814196545217015 30 18.612078977932637 20.513937282229968 36.70150987224158 24.17247386759582 31 33.83655102337059 22.717375526201188 26.418928726956015 17.0271447234722 32 18.59486137320366 19.175497169400494 25.620554507185368 36.60908695021048 33 20.322252866889244 22.789955000725794 36.73973000435477 20.14806212803019 34 31.934968790825955 20.10451444331543 24.197996806503124 23.762519959355494 35 31.818841631586587 21.454492669473073 27.58020031934969 19.14646537959065 36 23.980258382929307 34.93975903614458 21.759326462476412 19.3206561184497 37 23.022209319204528 34.37363913485266 22.151255624909275 20.45289592103353 38 16.301349978226156 24.067353752358834 39.91871098853244 19.712585280882568 39 16.228770503701554 24.125417331978515 25.83829293075918 33.807519233560754 40 16.881985774422994 19.219044854115257 43.518652924952825 20.380316446508928 41 16.606183771229496 18.725504427347946 26.346349252431413 38.321962548991145 42 30.84627667295689 17.709391784003483 30.323704456379737 21.120627086659894 43 15.764261866744084 15.69168239221948 42.110611119175495 26.433444621860936 44 19.45129917259399 33.69139207432138 25.98345187980839 20.873856873276235 45 14.835244592829147 21.178690666279575 43.24285092175933 20.74321381913195 46 30.120481927710845 24.647989548555667 24.299608070837568 20.931920452895923 47 14.080418057773262 21.207722456089417 29.597909711133692 35.113949775003626 48 18.885179271302075 18.202932210770793 27.4495572652054 35.46233125272173 49 28.05922485121208 19.91580780955146 24.86572797212948 27.15923936710698 50 13.543329946291188 19.988387284076065 40.25257657134562 26.215706198287126 51 19.99128919860627 19.16376306620209 22.865853658536587 37.97909407665505 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 1.5 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 2.5 14 3.0 15 3.0 16 3.0 17 2.0 18 1.0 19 1.0 20 1.0 21 1.5 22 2.0 23 2.5 24 3.0 25 2.5 26 4.0 27 6.0 28 14.0 29 22.0 30 32.5 31 43.0 32 63.0 33 83.0 34 99.5 35 116.0 36 196.0 37 276.0 38 306.0 39 336.0 40 387.5 41 439.0 42 565.0 43 691.0 44 692.5 45 694.0 46 680.0 47 666.0 48 660.5 49 655.0 50 640.0 51 625.0 52 1152.5 53 1680.0 54 946.0 55 212.0 56 161.0 57 110.0 58 80.5 59 51.0 60 49.5 61 48.0 62 35.0 63 22.0 64 28.0 65 34.0 66 29.5 67 25.0 68 20.5 69 16.0 70 12.5 71 9.0 72 5.0 73 1.0 74 1.0 75 2.0 76 3.0 77 2.5 78 2.0 79 2.0 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04354768471476266 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.014515894904920888 27 0.0 28 0.0 29 0.0 30 0.014515894904920888 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.014515894904920888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 6889.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.916969081143854 #Duplication Level Percentage of deduplicated Percentage of total 1 85.51953953347471 40.97837131659167 2 3.726143592850651 3.5709101466105384 3 2.5749772796122388 3.7015532007548266 4 1.8782187215995154 3.59994193642038 5 1.4843986670705847 3.5563942517056177 6 1.0905786125416541 3.135433299462912 7 0.7573462587094819 2.5402816083611555 8 0.3332323538321721 1.2773987516330383 9 0.5149954559224478 2.220931920452896 >10 2.029687973341412 15.343300914501379 >50 0.030293850348379277 0.9870808535346205 >100 0.030293850348379277 4.964436057482944 >500 0.030293850348379277 14.123965742488023 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 973 14.123965742488023 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG 342 4.964436057482944 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT 68 0.9870808535346205 No Hit GGTGATGTAACTCCTGTCTAGGTTCAGCCTACAGGAGCTTTTTGACATATC 43 0.6241834809115981 No Hit CTTGTATTCGTCGCCGTCGCAGATTTTTTGATTTCCGAAAATCCGACTTTC 40 0.5806357961968356 No Hit GGTACTGGCTCCAATTCACCTGTGGGTTTATGCGCACAGAAAAGCGATTTG 37 0.5370881114820728 No Hit GTGCTATGTGGGGATCTCAATGTGGCTCATGAAGAAATTGACCTCCGTAAC 37 0.5370881114820728 No Hit AGTGAATACAGCCGTACCAGAAAATCTCCATCACTTCGATTTTAGCAGGCA 34 0.49354042676731025 No Hit AGATAATGCAATCGAGACTCCCCGATAATTTCCTTGCCCTCCTCGAAAGAA 29 0.4209609522427058 No Hit GCATCAGGTCTAGTGCATTGGTCAACAGAGGGAAAGCGAGATGCAGTGAGA 28 0.40644505733778485 No Hit GGGTCTTACTATGATGCCTAGGCTAGTCTTGAACTCCTGGCCTCAAGTAAT 26 0.3774132675279431 No Hit CCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAG 24 0.3483814777181013 No Hit GTTCCAGGAGAGCCAGTGCCACACAAAGAAACCCTGTGTTGAAAAACCAAG 22 0.31934968790825957 No Hit ATATTAAGCTGCCACAGCAAGATAGAAACAAAGTCACAAAAAGCCACTAAG 21 0.3048337930033387 No Hit AGAACGACGATCGATCTTTTCTTTAAGCTCAGCAAACTTGCTGTCTCTTAT 20 0.2903178980984178 No Hit ACAAGAGACCGGTGTGATGATAAATGCTACTGACACAGGGGCTCAGAGTTG 20 0.2903178980984178 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 19 0.2758020031934969 No Hit GTCTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGC 19 0.2758020031934969 No Hit GTATTGGGACACTATTTCTCAGCACTTCCTGTGTCTGTATTGCTTAGACGG 18 0.261286108288576 No Hit GTGTCAAAGGAGGCAAAGAAGGGGGCACATGCTTTATTGATGCACTCAAAC 18 0.261286108288576 No Hit GCGCCAGAGCTGATGCATGCGGATGTTTTCTATGACAATCAATGGCATTCT 17 0.24677021338365512 No Hit GTATAAGAGACAGACAATGACCGAAGTCAAAATCCTGCAAAGCTTTAGCTG 16 0.2322543184787342 No Hit CAGAAAGTCCTTCGGGTTTTTGGTACGTTGACCATGTTTGCAGTAGAAGTG 16 0.2322543184787342 No Hit GTTTGGTGTCTGCCACATCTTTGCATCCTTCAATGACACCTTTGTCCATGT 16 0.2322543184787342 No Hit GCGCTGGATGCGACTTATAAAGTGAATGCGGTGAAAGAAAAATCTGTTGAA 15 0.21773842357381332 No Hit GGACGGCTACTATGGCTTCATTAATTTTTGTCTGTAGCTCTCTGAGCTCTT 15 0.21773842357381332 No Hit TAAGAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACC 15 0.21773842357381332 No Hit GTGTTGGCCTAATCCTTGACTAACTCCCTAAGTCTTTTTCGCATATGCAGC 14 0.20322252866889243 No Hit GTATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACCA 14 0.20322252866889243 No Hit CGGGTGCAGAGTCTATTCGTGACGTGATTGCTTTCCCAAAAACACAACGTG 14 0.20322252866889243 No Hit GCGTTCCTTCCAGGTCATTAGCTTCGGGCGCAAAAATACTTGGTGATCATC 13 0.18870663376397154 No Hit TGAACGTTCAATGCCGAGGCAGTCTAAAGCACCTGAGCGAATCAATGCTTC 13 0.18870663376397154 No Hit GAGATCATGATGACAGCCATTTTTATCTGTACCACCGGAGCTGTCTCTTAT 13 0.18870663376397154 No Hit ATGGTAGGTTGGTGAACCATGAATATCCTTCTGATAACTCATGACATTCAG 13 0.18870663376397154 No Hit ACTGAAGAATCTGGAACTTCAACCGTCGTGACATAAAACCATGTTCTTCTG 13 0.18870663376397154 No Hit CTATGGAGATGTTTACCTGGACATTTAAGAAAATTGGAGGATGGAGTTGGG 13 0.18870663376397154 No Hit TGTCCAAGCAGTACGTCATGGAGGATGAAACGGAGGTTAATCACGTCTGTC 13 0.18870663376397154 No Hit AGCTATTACATGGCAAAGATGTTATGACAGGGAATCCAGATGTTTGACTGG 12 0.17419073885905065 No Hit ATTGCCTTCTGCATTTTCAAAGTCAATAGGGTTCACTTTATGTGGCATCGC 12 0.17419073885905065 No Hit CCAGATACTACAAAAAGAGTGTTTCCAACCTGCTCTATGAAACGGAAGGTT 12 0.17419073885905065 No Hit ACTCCAATGTGAACCTAAGAAGACAAGAATCCAAGCTCAGAGACGGTGAAT 12 0.17419073885905065 No Hit CAGGGACAATACACCGATGGCATAACGTGAACCAGCAGCTGCGTTACTTGC 12 0.17419073885905065 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 12 0.17419073885905065 No Hit GCGCCAGAGCTGATTCATGCGGATGTTTTCTATGACAATCAATGGCATTCT 11 0.15967484395412979 No Hit CAAGCATACTGCCAGCCACCAGGATATCAAAGAGTGTGTCTGCTGTCTCTT 11 0.15967484395412979 No Hit ATCCATGGCGCGTCTCCTTATTTCAGGTGTGTACCCAATACTTGCAGGTAT 11 0.15967484395412979 No Hit GAGCGAATGGAGCATTTCCATTCTTAGCATTCAGAGGGTCACCAGCTCCAG 11 0.15967484395412979 No Hit CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTAT 11 0.15967484395412979 No Hit ATCCTGACGCCTCAAGTTTGATCAGGTTGTTGAATTTCTGGTTCACATCAT 11 0.15967484395412979 No Hit GAACTAGAAACGGAAACAGGCAGGTTAGCCGCAGCTTTTTAGGACTGTCTC 11 0.15967484395412979 No Hit ATGCTGCCTAACTGATGAAAACAAACTTCTCCACCCTTTCGTCTTTGGTCA 11 0.15967484395412979 No Hit TCACTACCCTCAACAACGTTCGAGTTCCTGCTCTATCTAAAACTCGATTGG 11 0.15967484395412979 No Hit GACAATGGTCCTCTGCATCTTCATCTTTGTCACGACACCAGACCTGTCTCT 11 0.15967484395412979 No Hit AAGGTTGATAACGGCAAAATGCAGGCACTGTCTCTTATACACATCTCCGAG 11 0.15967484395412979 No Hit GCTTTCTGCTAAGCGAGAAACGAGTGGAGAGAAATCCAGACAACTAAGGGA 11 0.15967484395412979 No Hit CCCTTAAGGGACCAGTTTAGCCTCAAAGACAGACAATTCAATGAGATTTGG 11 0.15967484395412979 No Hit GGTCCAGGCCTTGCAATGCTTTGTTATAAGCGTCTTTGGCTTCTTTCTCTT 11 0.15967484395412979 No Hit GACATACTAGGAGACCCAGACAACTACATACCAGCTAATCCACTAAACACC 11 0.15967484395412979 No Hit ATACAATCCTTACCGACAAAAACATTTCCGCAGCTGAGTATTCGTCTCTCA 11 0.15967484395412979 No Hit TAAAGGCTGGTAGGCGTCGGTATAGATTTTGTCGAACACCGGCCCCATGAC 11 0.15967484395412979 No Hit GGATAATAGCCTCGTTCTACCTGCCTTCTGATTAATATGTCTTGCGACATG 10 0.1451589490492089 No Hit CTGGTTTGCGACGCAAAACAAATTTACCGCTTTCTGTTCGCTGTAATACTG 10 0.1451589490492089 No Hit AAACAATGGTGCAGCTACCAGCTTGTAATTTTTAGGGACTGCAAACAAGGC 10 0.1451589490492089 No Hit CCACCGACAAACACATCCGACCAGATACCCAATAAAAACGTGCTAGTATTT 10 0.1451589490492089 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 10 0.1451589490492089 No Hit GTTTTCGTAGCCTTCAAATGCCATCGTCGGATTAATAACGCCAACATCAAC 10 0.1451589490492089 No Hit AGGCACAGCCATTCTTTTTTCAATCCATGCCTGGTTTAGGTTCAGGAGGGA 10 0.1451589490492089 No Hit ACCTTGTTATCAGTGATGGCTCTTCTCTGGAAGATCTTGTGGTCAAGAGCA 10 0.1451589490492089 No Hit AGATTGGCAAGCAATTGTCTCACAAGTGGAGACTGTCTCTTATACACATCT 10 0.1451589490492089 No Hit AGTTAGGACAGATAACTGAAGCTAAATGACTGGTTATCTGTCTGATATGCC 10 0.1451589490492089 No Hit CGTATAAACAATATGCGGCGCTCAATATTTTTTTCAATGTGCTGTATGCCT 9 0.130643054144288 No Hit GGAGTAGAACCACACGGCACACACACTAACACTCGTGGACTCGGCCGCAAA 9 0.130643054144288 No Hit CATGTGTGGTGAAAGGTCCATGTAGTCTGGGTGAACATCGGGGGCTGTCTC 9 0.130643054144288 No Hit CGGTATTCCAGTCTTACCAGACCCCTGCCTGCATTGGGAACATAGCTGTCT 9 0.130643054144288 No Hit AGCAAACAAAGAAGAAATCGAGCACCAGCATGTACATGGCTGTCTCTTATA 9 0.130643054144288 No Hit GTTTCGGCATTTTCCAGAATCCAGTTAACCGCACCAGTAAGGTGCTGTCTC 9 0.130643054144288 No Hit TTCCTAGGCTGCAGTTACCCTTACCAAGGGGTTAGAGGCCATCAATAGTCA 9 0.130643054144288 No Hit CCACTTGTACTTCTGGGCTTGCTGTATCACCTTCACTGCGTTGGTATTCTT 9 0.130643054144288 No Hit CAACCATCCGTAACGAGATCTGATTCCCTCTTCTGGAGTGTCTGAAGACCT 9 0.130643054144288 No Hit ACCTTGGCGTATTCGATATTACGTGCCTGGTTGGAAACACTATTCGTCCTG 9 0.130643054144288 No Hit GCTCAAATCACTAAACTCGAAGAAAGCCTAAAACTTGAAAAAGCACGTGCA 9 0.130643054144288 No Hit GCGCAGATGGGCTTGAAATACGTGGTCATTACCCTGTCTCTTATACACATC 9 0.130643054144288 No Hit ACTTAACACCGCTGCTCGCATAATAGAAGGTTGCCCTTGCGTAACATCTGT 9 0.130643054144288 No Hit TCGTATGAATCGCCTCAAACGGGCCAACATGACGTGGTGATTTGGGATACC 9 0.130643054144288 No Hit AGTGGACCCAATGTGTGGAACAGGGGCAATACCAATAGAGGGGGCTACTGA 9 0.130643054144288 No Hit TATCAACGCAGAGTACGGGGTGTGGTCTGAGATTGGAAAAGGCTTTCTAGA 9 0.130643054144288 No Hit GTGAGTGAATGAGCAGAGGACAAGAGGTACAAAATCGTGAGGAACTACCTT 9 0.130643054144288 No Hit ATCTTAGCATAGGTTCTTAGATTCTGAGTCGATCCGACAGCAACGATTGGA 8 0.1161271592393671 No Hit GGCTCAAGGACTTGCCAAGATTTTTACGCTGTTTAGTTATGGGAATGCCGG 8 0.1161271592393671 No Hit TTGCAATTTTATCCCCCTTTTCCAAGAAATTCTCAGTTGCTTGTTCAACAG 8 0.1161271592393671 No Hit GAATGGAATGGAACGGAATGGAGTGGAATGGAATCTGTCTCTTATACACAT 8 0.1161271592393671 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 8 0.1161271592393671 RNA PCR Primer, Index 23 (95% over 21bp) ATACCATAGGCAGCCGCAATGCTAATACCACTTTGCAATTACGCAATAACG 8 0.1161271592393671 No Hit GTACGCGCTCATAAGAATAAAAGTAGCGCCCTTTTTGCACGCAACGATCAC 8 0.1161271592393671 No Hit GCCCATTATCGCGAGAGGGAATCCATTTCGTCCCGTTTTTGTCTATCACCA 8 0.1161271592393671 No Hit GGCACTAACTTGATTTTAAATTGTTGTGCAAGGTCGATAAACGTTTGGTGG 8 0.1161271592393671 No Hit ATGTGGTTGCTGGGATTTGAACTTCGAACTTTTGGAAGAACAGTCGGGTGC 8 0.1161271592393671 No Hit TGTAAGTACACTGTAGCTGTCCTCAGACACTCCAGAAGAGGGAGTCAGATC 8 0.1161271592393671 No Hit AAATAACTCATGCCCTTTATGTGACCAGGAACGCTCTCTTCTTAGTTGCTG 7 0.10161126433444621 No Hit GCATCTCAGGAACGCGCCAACCAAACTGATGAAAGCGCTCGATTACGGTAA 7 0.10161126433444621 No Hit CTTTTGGGGTCAGGTATCCTGCATCAGTCATGGCTGTCTCTTATACACATC 7 0.10161126433444621 No Hit GTTGCAGATCAGCGACATCACGAGCGATATAAAAGCTGTCTCTTATACACA 7 0.10161126433444621 No Hit CTCAAAGTTACATTTTGGTACGCAAACGTGATATACCATCGCTGTCTCTTA 7 0.10161126433444621 No Hit AGGCTTTCCATTGGATTTTGTCCGAGGATCACTGCTTTTCTGGTAGCTCCT 7 0.10161126433444621 No Hit GTTTCTTCTCTTAAGCTTTGACCAGTTTCATCTTTGCAAGACGACAAGACA 7 0.10161126433444621 No Hit AAACCGTTTGATTACCTGTTAATCAACTGCACAAGAAAAGCCAGAATCTGT 7 0.10161126433444621 No Hit GATGGAAAATACTAGAATCTGGTGATCAGCAGCTCTGTCTCTTATACACAT 7 0.10161126433444621 No Hit CGTGAAGCATAACCGCCCACGGTTTCATTTCTTATCTCACCAGCGACACTA 7 0.10161126433444621 No Hit GACGTATGGAATTTTGCCTCCCTTTATCTGGACGTTGCCTGTCTCTTATAC 7 0.10161126433444621 No Hit CCTGAAGCCTGACTGGGGCAAGGGTTATTCAAGAAAAGCAGCAGCCCTTGA 7 0.10161126433444621 No Hit ATCCAGTTCTTTGTAGAACGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCA 7 0.10161126433444621 No Hit GTTTCAGCCAAACTGATTGATAAAGTTGGCCCTAAAGCTGTCTCTTATACA 7 0.10161126433444621 No Hit GAGTTAGTGGGACAAAACCATGTTTCTCGTGCATTAAGTCTGTCTCTTATA 7 0.10161126433444621 No Hit CTTAGCTTCTGCTGTTTTATCGCCTTTGTATTTTGCTGCAACATCTTTGTA 7 0.10161126433444621 No Hit AAACTGTACAGTGTCTCCAGCCTCTCCAACACATAGGCTGTCTCTTATACA 7 0.10161126433444621 No Hit GTGGTTTTGGTTCTCTTACCATCTTAGTTGGCCTGTTTTCACTGTCTCTTA 7 0.10161126433444621 No Hit CTGCCAGGACATACACCCCCATTCCCCTTCCCTACATCTCTACACCCTTTG 7 0.10161126433444621 No Hit GATAATGACAGTACCGGGAGAATAAGCTCCGGCTAACTCCTGTCTCTTATA 7 0.10161126433444621 No Hit AATCAGGTGGCACAGAAGCCTGGGTTGGTGAAAAAACCAGGTTGCCAGTAC 7 0.10161126433444621 No Hit CTTTGGACTCCCAAAATGCTGGGATTATAGGCATGAGCCACTGCCTGTCTC 7 0.10161126433444621 No Hit GTCACAGGGATGAAAATGCTGAAAATCAAATGCAATCCCATGCCTGTCTCT 7 0.10161126433444621 No Hit CAATTAGCAAATGCTACATATCAGGGCTCTCTGCCTCTGCTGTCTCTTATA 7 0.10161126433444621 No Hit CATCTGACCATGGCTTAACAGGTTTGGCAGGTTTACGTCTGTCTCTTATAC 7 0.10161126433444621 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.15967484395412979 2 0.0 0.0 0.31934968790825957 3 0.0 0.0 0.3338655828131804 4 0.0 0.0 0.5951516911017565 5 0.0 0.0 0.7112788503411235 6 0.0 0.0 0.7548265350558861 7 0.0 0.0 0.769342429960807 8 0.0 0.0 0.9290172739149368 9 0.0 0.0 1.1467556974887503 10 0.0 0.0 1.2628828567281174 11 0.0 0.0 1.291914646537959 12 0.0 0.0 1.3209464363478007 13 0.0 0.0 1.408041805777326 14 0.0 0.0 1.4661053853970096 15 0.0 0.0 1.5241689650166932 16 0.0 0.0 1.5677166497314559 17 0.0 0.0 1.5967484395412976 18 0.0 0.0 1.6548120191609812 19 0.0 0.0 1.669327914065902 20 0.0 0.0 1.683843808970823 21 0.0 0.0 1.7854550733052692 22 0.0 0.0 1.9596458121643199 23 0.0 0.0 1.9596458121643199 24 0.0 0.0 2.1483524459282917 25 0.0 0.0 2.3080272898824212 26 0.0 0.0 2.45318623893163 27 0.0 0.0 2.525765713456235 28 0.0 0.0 2.816083611554652 29 0.0 0.0 3.0193061402235446 30 0.0 0.0 3.4257511975613295 31 0.0 0.0 4.137030047902453 32 0.0 0.0 4.862824793148498 33 0.0 0.0 6.0821599651618525 34 0.0 0.0 7.18536797793584 35 0.0 0.0 8.462766729568878 36 0.0 0.0 9.841776745536363 37 0.0 0.0 11.046596022644795 38 0.0 0.0 12.759471621425462 39 0.0 0.0 14.733633328494701 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 135 0.0 43.333332 45 ATGCCGT 145 0.0 40.34483 44 GTATGCC 205 0.0 28.536585 42 ATCTCGT 205 0.0 28.536585 37 TATGCCG 205 0.0 28.536585 43 TCTCGTA 205 0.0 28.536585 38 GAATCTC 175 0.0 28.285713 35 AATCTCG 185 0.0 27.972973 36 CGTATGC 210 0.0 27.857143 41 TCGTATG 210 0.0 27.857143 40 CTCGTAT 210 0.0 27.857143 39 GGAATCT 180 0.0 27.500002 34 CTGGAAT 185 0.0 26.756756 32 ACTTGCT 190 0.0 26.052631 27 GCTGGAA 190 0.0 26.052631 31 TGCTGGA 190 0.0 26.052631 30 CTTGCTG 190 0.0 26.052631 28 TGGAATC 190 0.0 26.052631 33 AGACTTG 190 0.0 26.052631 25 GACTTGC 190 0.0 26.052631 26 >>END_MODULE