FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_75.341000000066c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_75.341000000066c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC97314.123965742488023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCG3424.964436057482944No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGT680.9870808535346205No Hit
GGTGATGTAACTCCTGTCTAGGTTCAGCCTACAGGAGCTTTTTGACATATC430.6241834809115981No Hit
CTTGTATTCGTCGCCGTCGCAGATTTTTTGATTTCCGAAAATCCGACTTTC400.5806357961968356No Hit
GGTACTGGCTCCAATTCACCTGTGGGTTTATGCGCACAGAAAAGCGATTTG370.5370881114820728No Hit
GTGCTATGTGGGGATCTCAATGTGGCTCATGAAGAAATTGACCTCCGTAAC370.5370881114820728No Hit
AGTGAATACAGCCGTACCAGAAAATCTCCATCACTTCGATTTTAGCAGGCA340.49354042676731025No Hit
AGATAATGCAATCGAGACTCCCCGATAATTTCCTTGCCCTCCTCGAAAGAA290.4209609522427058No Hit
GCATCAGGTCTAGTGCATTGGTCAACAGAGGGAAAGCGAGATGCAGTGAGA280.40644505733778485No Hit
GGGTCTTACTATGATGCCTAGGCTAGTCTTGAACTCCTGGCCTCAAGTAAT260.3774132675279431No Hit
CCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAG240.3483814777181013No Hit
GTTCCAGGAGAGCCAGTGCCACACAAAGAAACCCTGTGTTGAAAAACCAAG220.31934968790825957No Hit
ATATTAAGCTGCCACAGCAAGATAGAAACAAAGTCACAAAAAGCCACTAAG210.3048337930033387No Hit
AGAACGACGATCGATCTTTTCTTTAAGCTCAGCAAACTTGCTGTCTCTTAT200.2903178980984178No Hit
ACAAGAGACCGGTGTGATGATAAATGCTACTGACACAGGGGCTCAGAGTTG200.2903178980984178No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC190.2758020031934969No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGC190.2758020031934969No Hit
GTATTGGGACACTATTTCTCAGCACTTCCTGTGTCTGTATTGCTTAGACGG180.261286108288576No Hit
GTGTCAAAGGAGGCAAAGAAGGGGGCACATGCTTTATTGATGCACTCAAAC180.261286108288576No Hit
GCGCCAGAGCTGATGCATGCGGATGTTTTCTATGACAATCAATGGCATTCT170.24677021338365512No Hit
GTATAAGAGACAGACAATGACCGAAGTCAAAATCCTGCAAAGCTTTAGCTG160.2322543184787342No Hit
CAGAAAGTCCTTCGGGTTTTTGGTACGTTGACCATGTTTGCAGTAGAAGTG160.2322543184787342No Hit
GTTTGGTGTCTGCCACATCTTTGCATCCTTCAATGACACCTTTGTCCATGT160.2322543184787342No Hit
GCGCTGGATGCGACTTATAAAGTGAATGCGGTGAAAGAAAAATCTGTTGAA150.21773842357381332No Hit
GGACGGCTACTATGGCTTCATTAATTTTTGTCTGTAGCTCTCTGAGCTCTT150.21773842357381332No Hit
TAAGAGCACTGACTGCTCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACC150.21773842357381332No Hit
GTGTTGGCCTAATCCTTGACTAACTCCCTAAGTCTTTTTCGCATATGCAGC140.20322252866889243No Hit
GTATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACCA140.20322252866889243No Hit
CGGGTGCAGAGTCTATTCGTGACGTGATTGCTTTCCCAAAAACACAACGTG140.20322252866889243No Hit
GCGTTCCTTCCAGGTCATTAGCTTCGGGCGCAAAAATACTTGGTGATCATC130.18870663376397154No Hit
TGAACGTTCAATGCCGAGGCAGTCTAAAGCACCTGAGCGAATCAATGCTTC130.18870663376397154No Hit
GAGATCATGATGACAGCCATTTTTATCTGTACCACCGGAGCTGTCTCTTAT130.18870663376397154No Hit
ATGGTAGGTTGGTGAACCATGAATATCCTTCTGATAACTCATGACATTCAG130.18870663376397154No Hit
ACTGAAGAATCTGGAACTTCAACCGTCGTGACATAAAACCATGTTCTTCTG130.18870663376397154No Hit
CTATGGAGATGTTTACCTGGACATTTAAGAAAATTGGAGGATGGAGTTGGG130.18870663376397154No Hit
TGTCCAAGCAGTACGTCATGGAGGATGAAACGGAGGTTAATCACGTCTGTC130.18870663376397154No Hit
AGCTATTACATGGCAAAGATGTTATGACAGGGAATCCAGATGTTTGACTGG120.17419073885905065No Hit
ATTGCCTTCTGCATTTTCAAAGTCAATAGGGTTCACTTTATGTGGCATCGC120.17419073885905065No Hit
CCAGATACTACAAAAAGAGTGTTTCCAACCTGCTCTATGAAACGGAAGGTT120.17419073885905065No Hit
ACTCCAATGTGAACCTAAGAAGACAAGAATCCAAGCTCAGAGACGGTGAAT120.17419073885905065No Hit
CAGGGACAATACACCGATGGCATAACGTGAACCAGCAGCTGCGTTACTTGC120.17419073885905065No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC120.17419073885905065No Hit
GCGCCAGAGCTGATTCATGCGGATGTTTTCTATGACAATCAATGGCATTCT110.15967484395412979No Hit
CAAGCATACTGCCAGCCACCAGGATATCAAAGAGTGTGTCTGCTGTCTCTT110.15967484395412979No Hit
ATCCATGGCGCGTCTCCTTATTTCAGGTGTGTACCCAATACTTGCAGGTAT110.15967484395412979No Hit
GAGCGAATGGAGCATTTCCATTCTTAGCATTCAGAGGGTCACCAGCTCCAG110.15967484395412979No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTAT110.15967484395412979No Hit
ATCCTGACGCCTCAAGTTTGATCAGGTTGTTGAATTTCTGGTTCACATCAT110.15967484395412979No Hit
GAACTAGAAACGGAAACAGGCAGGTTAGCCGCAGCTTTTTAGGACTGTCTC110.15967484395412979No Hit
ATGCTGCCTAACTGATGAAAACAAACTTCTCCACCCTTTCGTCTTTGGTCA110.15967484395412979No Hit
TCACTACCCTCAACAACGTTCGAGTTCCTGCTCTATCTAAAACTCGATTGG110.15967484395412979No Hit
GACAATGGTCCTCTGCATCTTCATCTTTGTCACGACACCAGACCTGTCTCT110.15967484395412979No Hit
AAGGTTGATAACGGCAAAATGCAGGCACTGTCTCTTATACACATCTCCGAG110.15967484395412979No Hit
GCTTTCTGCTAAGCGAGAAACGAGTGGAGAGAAATCCAGACAACTAAGGGA110.15967484395412979No Hit
CCCTTAAGGGACCAGTTTAGCCTCAAAGACAGACAATTCAATGAGATTTGG110.15967484395412979No Hit
GGTCCAGGCCTTGCAATGCTTTGTTATAAGCGTCTTTGGCTTCTTTCTCTT110.15967484395412979No Hit
GACATACTAGGAGACCCAGACAACTACATACCAGCTAATCCACTAAACACC110.15967484395412979No Hit
ATACAATCCTTACCGACAAAAACATTTCCGCAGCTGAGTATTCGTCTCTCA110.15967484395412979No Hit
TAAAGGCTGGTAGGCGTCGGTATAGATTTTGTCGAACACCGGCCCCATGAC110.15967484395412979No Hit
GGATAATAGCCTCGTTCTACCTGCCTTCTGATTAATATGTCTTGCGACATG100.1451589490492089No Hit
CTGGTTTGCGACGCAAAACAAATTTACCGCTTTCTGTTCGCTGTAATACTG100.1451589490492089No Hit
AAACAATGGTGCAGCTACCAGCTTGTAATTTTTAGGGACTGCAAACAAGGC100.1451589490492089No Hit
CCACCGACAAACACATCCGACCAGATACCCAATAAAAACGTGCTAGTATTT100.1451589490492089No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC100.1451589490492089No Hit
GTTTTCGTAGCCTTCAAATGCCATCGTCGGATTAATAACGCCAACATCAAC100.1451589490492089No Hit
AGGCACAGCCATTCTTTTTTCAATCCATGCCTGGTTTAGGTTCAGGAGGGA100.1451589490492089No Hit
ACCTTGTTATCAGTGATGGCTCTTCTCTGGAAGATCTTGTGGTCAAGAGCA100.1451589490492089No Hit
AGATTGGCAAGCAATTGTCTCACAAGTGGAGACTGTCTCTTATACACATCT100.1451589490492089No Hit
AGTTAGGACAGATAACTGAAGCTAAATGACTGGTTATCTGTCTGATATGCC100.1451589490492089No Hit
CGTATAAACAATATGCGGCGCTCAATATTTTTTTCAATGTGCTGTATGCCT90.130643054144288No Hit
GGAGTAGAACCACACGGCACACACACTAACACTCGTGGACTCGGCCGCAAA90.130643054144288No Hit
CATGTGTGGTGAAAGGTCCATGTAGTCTGGGTGAACATCGGGGGCTGTCTC90.130643054144288No Hit
CGGTATTCCAGTCTTACCAGACCCCTGCCTGCATTGGGAACATAGCTGTCT90.130643054144288No Hit
AGCAAACAAAGAAGAAATCGAGCACCAGCATGTACATGGCTGTCTCTTATA90.130643054144288No Hit
GTTTCGGCATTTTCCAGAATCCAGTTAACCGCACCAGTAAGGTGCTGTCTC90.130643054144288No Hit
TTCCTAGGCTGCAGTTACCCTTACCAAGGGGTTAGAGGCCATCAATAGTCA90.130643054144288No Hit
CCACTTGTACTTCTGGGCTTGCTGTATCACCTTCACTGCGTTGGTATTCTT90.130643054144288No Hit
CAACCATCCGTAACGAGATCTGATTCCCTCTTCTGGAGTGTCTGAAGACCT90.130643054144288No Hit
ACCTTGGCGTATTCGATATTACGTGCCTGGTTGGAAACACTATTCGTCCTG90.130643054144288No Hit
GCTCAAATCACTAAACTCGAAGAAAGCCTAAAACTTGAAAAAGCACGTGCA90.130643054144288No Hit
GCGCAGATGGGCTTGAAATACGTGGTCATTACCCTGTCTCTTATACACATC90.130643054144288No Hit
ACTTAACACCGCTGCTCGCATAATAGAAGGTTGCCCTTGCGTAACATCTGT90.130643054144288No Hit
TCGTATGAATCGCCTCAAACGGGCCAACATGACGTGGTGATTTGGGATACC90.130643054144288No Hit
AGTGGACCCAATGTGTGGAACAGGGGCAATACCAATAGAGGGGGCTACTGA90.130643054144288No Hit
TATCAACGCAGAGTACGGGGTGTGGTCTGAGATTGGAAAAGGCTTTCTAGA90.130643054144288No Hit
GTGAGTGAATGAGCAGAGGACAAGAGGTACAAAATCGTGAGGAACTACCTT90.130643054144288No Hit
ATCTTAGCATAGGTTCTTAGATTCTGAGTCGATCCGACAGCAACGATTGGA80.1161271592393671No Hit
GGCTCAAGGACTTGCCAAGATTTTTACGCTGTTTAGTTATGGGAATGCCGG80.1161271592393671No Hit
TTGCAATTTTATCCCCCTTTTCCAAGAAATTCTCAGTTGCTTGTTCAACAG80.1161271592393671No Hit
GAATGGAATGGAACGGAATGGAGTGGAATGGAATCTGTCTCTTATACACAT80.1161271592393671No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC80.1161271592393671RNA PCR Primer, Index 23 (95% over 21bp)
ATACCATAGGCAGCCGCAATGCTAATACCACTTTGCAATTACGCAATAACG80.1161271592393671No Hit
GTACGCGCTCATAAGAATAAAAGTAGCGCCCTTTTTGCACGCAACGATCAC80.1161271592393671No Hit
GCCCATTATCGCGAGAGGGAATCCATTTCGTCCCGTTTTTGTCTATCACCA80.1161271592393671No Hit
GGCACTAACTTGATTTTAAATTGTTGTGCAAGGTCGATAAACGTTTGGTGG80.1161271592393671No Hit
ATGTGGTTGCTGGGATTTGAACTTCGAACTTTTGGAAGAACAGTCGGGTGC80.1161271592393671No Hit
TGTAAGTACACTGTAGCTGTCCTCAGACACTCCAGAAGAGGGAGTCAGATC80.1161271592393671No Hit
AAATAACTCATGCCCTTTATGTGACCAGGAACGCTCTCTTCTTAGTTGCTG70.10161126433444621No Hit
GCATCTCAGGAACGCGCCAACCAAACTGATGAAAGCGCTCGATTACGGTAA70.10161126433444621No Hit
CTTTTGGGGTCAGGTATCCTGCATCAGTCATGGCTGTCTCTTATACACATC70.10161126433444621No Hit
GTTGCAGATCAGCGACATCACGAGCGATATAAAAGCTGTCTCTTATACACA70.10161126433444621No Hit
CTCAAAGTTACATTTTGGTACGCAAACGTGATATACCATCGCTGTCTCTTA70.10161126433444621No Hit
AGGCTTTCCATTGGATTTTGTCCGAGGATCACTGCTTTTCTGGTAGCTCCT70.10161126433444621No Hit
GTTTCTTCTCTTAAGCTTTGACCAGTTTCATCTTTGCAAGACGACAAGACA70.10161126433444621No Hit
AAACCGTTTGATTACCTGTTAATCAACTGCACAAGAAAAGCCAGAATCTGT70.10161126433444621No Hit
GATGGAAAATACTAGAATCTGGTGATCAGCAGCTCTGTCTCTTATACACAT70.10161126433444621No Hit
CGTGAAGCATAACCGCCCACGGTTTCATTTCTTATCTCACCAGCGACACTA70.10161126433444621No Hit
GACGTATGGAATTTTGCCTCCCTTTATCTGGACGTTGCCTGTCTCTTATAC70.10161126433444621No Hit
CCTGAAGCCTGACTGGGGCAAGGGTTATTCAAGAAAAGCAGCAGCCCTTGA70.10161126433444621No Hit
ATCCAGTTCTTTGTAGAACGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCA70.10161126433444621No Hit
GTTTCAGCCAAACTGATTGATAAAGTTGGCCCTAAAGCTGTCTCTTATACA70.10161126433444621No Hit
GAGTTAGTGGGACAAAACCATGTTTCTCGTGCATTAAGTCTGTCTCTTATA70.10161126433444621No Hit
CTTAGCTTCTGCTGTTTTATCGCCTTTGTATTTTGCTGCAACATCTTTGTA70.10161126433444621No Hit
AAACTGTACAGTGTCTCCAGCCTCTCCAACACATAGGCTGTCTCTTATACA70.10161126433444621No Hit
GTGGTTTTGGTTCTCTTACCATCTTAGTTGGCCTGTTTTCACTGTCTCTTA70.10161126433444621No Hit
CTGCCAGGACATACACCCCCATTCCCCTTCCCTACATCTCTACACCCTTTG70.10161126433444621No Hit
GATAATGACAGTACCGGGAGAATAAGCTCCGGCTAACTCCTGTCTCTTATA70.10161126433444621No Hit
AATCAGGTGGCACAGAAGCCTGGGTTGGTGAAAAAACCAGGTTGCCAGTAC70.10161126433444621No Hit
CTTTGGACTCCCAAAATGCTGGGATTATAGGCATGAGCCACTGCCTGTCTC70.10161126433444621No Hit
GTCACAGGGATGAAAATGCTGAAAATCAAATGCAATCCCATGCCTGTCTCT70.10161126433444621No Hit
CAATTAGCAAATGCTACATATCAGGGCTCTCTGCCTCTGCTGTCTCTTATA70.10161126433444621No Hit
CATCTGACCATGGCTTAACAGGTTTGGCAGGTTTACGTCTGTCTCTTATAC70.10161126433444621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1350.043.33333245
ATGCCGT1450.040.3448344
GTATGCC2050.028.53658542
ATCTCGT2050.028.53658537
TATGCCG2050.028.53658543
TCTCGTA2050.028.53658538
GAATCTC1750.028.28571335
AATCTCG1850.027.97297336
CGTATGC2100.027.85714341
TCGTATG2100.027.85714340
CTCGTAT2100.027.85714339
GGAATCT1800.027.50000234
CTGGAAT1850.026.75675632
ACTTGCT1900.026.05263127
GCTGGAA1900.026.05263131
TGCTGGA1900.026.05263130
CTTGCTG1900.026.05263128
TGGAATC1900.026.05263133
AGACTTG1900.026.05263125
GACTTGC1900.026.05263126
Produced by FastQC (version 0.11.2)