FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_74.341000000066b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_74.341000000066b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247999
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC4940.19919435159012738No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC3980.1604845180827342No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA3860.15564578889431005No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATAC3480.14032314646430027No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA3220.12983923322271462No Hit
GATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCG2770.11169399876612406No Hit
GCGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATC2570.10362945011875048No Hit
CGTTGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCT2480.10000040322743237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT552.730303E-936.81785644
TTCCGAC250.00210402235.999683
ATTGCGT250.00210402235.9996816
TGCCGTC606.3901098E-933.74969545
GGTCTAC406.14547E-428.130421
ACTTGGG450.001223906624.99977732
CGGGCTC450.001223906624.99977717
AGGATTA2000.024.7497795
CGGGACA551.3640632E-424.54523717
GATTAGT2050.023.0485747
CTAGGAT2050.023.0485743
CTCTGGT500.002257111522.5043372
CTCGTAT903.2657408E-722.49979839
AGTGTAC1600.022.49979827
GCTAGGA2050.021.955452
ATTAGTC2200.021.4770818
TAGGATT2200.021.4770814
GGATTAG2200.021.4770816
TAGTCCC2200.021.47708110
CGTATGC955.496204E-721.31559841