FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_71.3410000000668e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_71.3410000000668e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495683
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC6260.12629039123794844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1350.034.9986743
ATATCTC1050.034.2878735
CGCTATA1000.033.74871431
GCTATAT1050.032.14163232
GACGCTA1050.032.14163229
AACGTCG300.005145425429.9988622
CGACGCT1200.029.9988628
TCGTATG1600.029.53310440
CGTATGC1600.029.53012341
CCACGAG1600.029.53012320
CTATATC1200.028.1239333
CTCGTAT1700.027.79586239
ACGACGC1300.027.69125227
TATATCT1250.027.00169434
TCTCGTA1750.027.00169438
GTACGCT507.152179E-526.9989723
TATCTCG1350.026.6683436
ACGCTAT1350.026.66565130
GCCCACG1800.026.25164818
CGAGACG1550.026.12803623
Produced by FastQC (version 0.11.2)