FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_7.34100000006282.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_7.34100000006282.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2029
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC251.232134056185313No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC241.1828486939379004No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC211.0349926071956628No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC150.7392804337111878RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC120.5914243469689502RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC110.5421389847215378No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC110.5421389847215378No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC110.5421389847215378No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC100.4928536224741252RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC80.39428289797930016No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC70.34499753573188763No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC60.2957121734844751No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC60.2957121734844751No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGT40.19714144898965008No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC40.19714144898965008No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC40.19714144898965008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.14785608674223755No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT30.14785608674223755No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC30.14785608674223755No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT30.14785608674223755No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG205.490732E-444.97500210
CATCTCC205.490732E-444.9750029
TCTCCGA205.490732E-444.97500211
AGCCCAC250.001648689735.9817
GTATGCC250.001648689735.9842
ATCTCGT250.001648689735.9837
GCCCACG250.001648689735.9818
TGCCGTC250.001648689735.9845
CCACGAG250.001648689735.9820
GAGCCCA250.001648689735.9816
CACGAGA250.001648689735.9821
CCCACGA250.001648689735.9819
TCTCGTA250.001648689735.9838
ACGAGAC250.001648689735.9822
ATGCCGT250.001648689735.9844
CGTATGC250.001648689735.9841
CGAGCCC250.001648689735.9815
CCGAGCC250.001648689735.9814
TCGTATG250.001648689735.9840
TCCGAGC250.001648689735.9813
Produced by FastQC (version 0.11.2)