FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_69.34100000006664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_69.34100000006664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470390
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATA700.038.57097231
TATATCT900.032.4996234
CTATATC1000.031.49962833
CGAGACG1250.028.79965823
ACGCTAT952.3646862E-1128.42071730
GCTATAT1004.5474735E-1126.99968132
TATGCCG1250.026.9996843
TCGTATG1350.026.66635140
TAGGTAG608.178855E-626.24969132
TCTCGTA1400.025.71398238
CTCGTAT1450.024.82729339
CGTCTAT551.3617105E-424.5582181
CGACGCT1101.5097612E-1024.54516428
AGACGAC1101.5097612E-1024.54516425
GACGACG1101.5097612E-1024.54516426
GAGACGA1301.8189894E-1224.23048424
TATCTCG1301.8189894E-1224.23048436
CCCACGA1500.023.99971619
CCACGAG1500.023.99971620
GACGCTA1152.6375346E-1023.47798329
Produced by FastQC (version 0.11.2)