FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_68.34100000006658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_68.34100000006658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516754
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC950.035.52486445
ACGTGTG300.005145694629.9987755
TATGCCG1150.029.34662643
CGCTATA702.8809154E-828.9273931
TATCTCG1004.5474735E-1126.99889836
TCGTATG1300.025.96047640
ACGAGAC1300.025.96047622
CGACGCT801.0499025E-725.31636428
ACGACGC801.0499025E-725.31636427
GCTATAT801.051867E-725.31146432
GAGCGAA450.001225933124.9989816
ATGCCGT1350.024.99897844
CTATATC952.1105734E-823.68324333
CGAGACG1052.3610482E-923.57046523
GGTATCA5100.023.3927151
CTCGTAT1450.023.27491239
ACGCTAT903.2748721E-722.50343530
GCCCACG1500.022.4990818
GTATCAA9200.021.7759511
CACGAGA1601.8189894E-1221.09288621
Produced by FastQC (version 0.11.2)