FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_67.3410000000664b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_67.3410000000664b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402992
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC5870.1456604597609878No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTAA250.002105631235.99950421
CTATACT401.5582096E-533.7495354
GCTATAT751.6971171E-929.99958632
CGCTATA751.6971171E-929.99958631
TATGCCG1001.8189894E-1229.24959643
TATCTCG852.0190782E-1029.11724736
GACGACG852.0190782E-1029.11724726
CTATATC803.3815013E-928.12461333
CCACGAG1053.6379788E-1227.85675820
CGACGCT1004.5474735E-1126.99962628
CTCGTAT1105.456968E-1226.59054239
ACGCTAT856.4555934E-926.47022430
ACGACGC957.385097E-1026.0522727
GCCCACG1159.094947E-1225.43443118
TGCCGTC1159.094947E-1225.43443145
CGAGACG1101.5097612E-1024.54511623
ATGCCGT1201.6370905E-1124.37466444
TCTCGTA1252.910383E-1123.39967738
ACGAGAC1252.910383E-1123.39967722
CGTATGC1305.0931703E-1122.49969141
Produced by FastQC (version 0.11.2)