FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_64.34100000006614.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_64.34100000006614.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93988
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC1114911.862152615227476No Hit
TTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTCT1440.15321104821892156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC12900.043.25581745
ATGCCGT13000.042.92307744
CGTATGC13200.042.61363641
CTCGTAT13150.042.6045639
TATGCCG13150.042.6045643
TCTCGTA13150.042.6045638
TCGTATG13150.042.6045640
AATCTCG12800.042.53906236
GTATGCC13200.042.4431842
GGCGAAT12650.042.33201632
CGAGACC13100.042.25190723
CAAGGCG12850.042.19844429
GCGAATC12700.042.16535633
GAATCTC12900.042.03488535
AGGCGAA12750.042.031
CGAATCT12750.042.034
ACGAGAC13450.041.98884622
ATCTCGT13350.041.96629337
GACCAAG13000.041.88461726
AAGGCGA12800.041.83593830