FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_63.34100000006607.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_63.34100000006607.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433657
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC18290.42176189938130826No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2250.040.9984245
GCCCACG2500.037.79854618
TATGCCG2500.036.89858243
CTCGTAT2700.036.66525739
GCGAATC2300.036.1942633
AATCTCG2550.036.17508336
CGTATGC2600.035.47940441
GGCGAAT2300.035.21603832
AGGCGAA2350.034.4667631
CCCACGA2750.034.36231219
TCGTATG2800.033.74870740
ACGAGAC2800.033.74870722
ATGCCGT2800.032.94516444
TCTCGTA2950.032.7953538
CCACGAG3000.032.2487620
CGACCCG352.818135E-432.1416245
CGAGACC2600.032.01823
CAAGGCG2550.031.76714929
CGAGCCC3000.031.49878915
CACGAGA3200.030.9363121
Produced by FastQC (version 0.11.2)