FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_62.341000000065f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_62.341000000065f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437408
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC10250.23433499158680227No Hit
CCCCTACCCATCCAGCAAAACTGAATGCCACGGTTTCGGCGGTGTACTTGA10160.23227741605091815No Hit
TCCTAGACCCGAAGCGGAGTGATCTACCCATGGCCAGGTTGAAGCGCGTGT6740.1540895456873217No Hit
CAAGTACACCGCCGAAACCGTGGCATTCAGTTTTGCTGGATGGGTAGGGGA6720.1536323066793474No Hit
GGGTAGGGGAGCGTCGTTCACGAGGTGAAGCCAGCGGGTAACTTCTGGTGG6270.14334442899992683No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACTC5880.134428268344429No Hit
GGTCTAGGACACGCGACTCAATCGCCCTATTCAGACTCGCTTTCGCTACGG5550.1268838247128539No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGACA4750.10859426439388396No Hit
ACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACGG4670.10676530836198697No Hit
CTCTTACACGCGCTTCAACCTGGCCATGGGTAGATCACTCCGCTTCGGGTC4640.10607944985002561No Hit
GCCCAGACCACCGGTTAAGGCCCCTAAGCGTGTGCTAAGTGGGAAAGGATG4620.10562221084205135No Hit
GGTCTGGGCTGTTTCCCTCTCGACTATGAAGCTTATCCCCCACAGTCTCAC4530.10356463530616725No Hit
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACG4510.10310739629819299No Hit
CCCCTACCCACAGCTCATCCCCTCCATTTTCAACTGAAGTGGGTTCGGTCC4420.10104982076230887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1900.039.07626738
TCGTATG1900.039.07626740
CTCGTAT1950.038.0743139
CGTATGC2150.036.62539341
GGCGAAT1750.035.9975332
CACGAGA2150.035.57895321
ACGAGAC2150.035.57895322
GCGAATC1800.034.997633
ATCTCGT2150.033.48607337
AATCTCG2000.032.62276536
CGAATCT1950.032.30547734
TATGCCG2550.030.88023643
AGGCGAA2050.030.729631
GCCGCGT300.005145539529.9979448
GTATGCC2800.029.73010342
CGAGACC2350.029.67881623
GCCCACG2750.029.45252618
ATGCCGT2700.029.16466744
CCACGAG2850.028.41910420
TGCCGTC2750.027.81627545
Produced by FastQC (version 0.11.2)