##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 24h_61.341000000065ec.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4705 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.438894792773645 38.0 32.0 38.0 27.0 38.0 2 34.83209351753454 38.0 32.0 38.0 32.0 38.0 3 34.79256110520723 38.0 32.0 38.0 32.0 38.0 4 35.11562167906482 38.0 32.0 38.0 32.0 38.0 5 35.096280552603616 38.0 32.0 38.0 32.0 38.0 6 36.551753453772584 38.0 38.0 40.0 32.0 40.0 7 36.2663124335813 38.0 38.0 40.0 32.0 40.0 8 36.19596174282678 38.0 38.0 40.0 32.0 40.0 9 36.6221041445271 38.0 38.0 40.0 32.0 40.0 10 36.382571732199786 38.0 38.0 40.0 32.0 40.0 11 36.41126461211477 38.0 38.0 40.0 32.0 40.0 12 36.11200850159405 38.0 38.0 40.0 32.0 40.0 13 36.194686503719446 38.0 38.0 40.0 32.0 40.0 14 35.94261424017003 38.0 38.0 40.0 27.0 40.0 15 36.02635494155154 38.0 38.0 40.0 27.0 40.0 16 35.720722635494155 38.0 38.0 40.0 27.0 40.0 17 35.769819341126464 38.0 38.0 40.0 27.0 40.0 18 35.67821466524973 38.0 38.0 40.0 27.0 40.0 19 35.89628055260361 38.0 38.0 40.0 27.0 40.0 20 35.86014877789586 38.0 38.0 40.0 27.0 40.0 21 35.87906482465462 38.0 38.0 40.0 27.0 40.0 22 35.94856535600425 38.0 38.0 40.0 27.0 40.0 23 36.0769394261424 38.0 38.0 40.0 32.0 40.0 24 35.90180658873539 38.0 38.0 40.0 27.0 40.0 25 35.782359192348565 38.0 38.0 40.0 27.0 40.0 26 35.75154091392136 38.0 38.0 40.0 27.0 40.0 27 35.60467587672689 38.0 38.0 40.0 27.0 40.0 28 35.58469713071201 38.0 38.0 40.0 27.0 40.0 29 35.68501594048884 38.0 38.0 40.0 27.0 40.0 30 35.510945802337936 38.0 38.0 40.0 27.0 40.0 31 35.539001062699256 38.0 38.0 40.0 27.0 40.0 32 35.715834218916044 38.0 38.0 40.0 27.0 40.0 33 35.373007438894795 38.0 38.0 40.0 27.0 40.0 34 35.43549415515409 38.0 38.0 40.0 27.0 40.0 35 35.592561105207224 38.0 38.0 40.0 27.0 40.0 36 35.531774707757705 38.0 38.0 40.0 27.0 40.0 37 35.38490967056323 38.0 38.0 40.0 27.0 40.0 38 35.55621679064825 38.0 38.0 40.0 27.0 40.0 39 35.29670563230606 38.0 32.0 40.0 27.0 40.0 40 35.49096705632306 38.0 38.0 40.0 27.0 40.0 41 35.46376195536663 38.0 32.0 40.0 27.0 40.0 42 35.398299681190224 38.0 32.0 40.0 27.0 40.0 43 35.663336875664186 38.0 38.0 40.0 27.0 40.0 44 35.46865037194474 38.0 38.0 40.0 27.0 40.0 45 35.61700318809777 38.0 38.0 40.0 27.0 40.0 46 35.3317747077577 38.0 32.0 40.0 27.0 40.0 47 35.29606801275239 38.0 32.0 40.0 27.0 40.0 48 35.35600425079702 38.0 32.0 40.0 27.0 40.0 49 35.12922422954304 38.0 32.0 40.0 27.0 40.0 50 35.221891604675875 38.0 32.0 40.0 27.0 40.0 51 34.43931987247609 38.0 32.0 38.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.31205864448051557 2201 2 -0.16321248196248206 2201 3 -0.2733061643217951 2201 4 0.3137293695887422 2201 5 2.2226072104978414 2201 6 2.16200565927128 2201 7 2.1303441783910557 2201 8 3.1089635191197615 2201 9 2.24979031385282 2201 10 2.4388979076479202 2201 11 1.231148538961044 2201 12 1.1326800369769074 2201 13 -1.128779987373754 2201 14 -1.669903273809517 2201 15 0.7328034812409925 2201 16 -0.3829100153318947 2201 17 -0.30591348755411474 2201 18 -1.0372362012986969 2201 19 1.1163087346681024 2201 20 2.8733067279942333 2201 21 -0.029565746753242195 2201 22 2.1686885597041936 2201 23 1.8175211940837102 2201 24 2.290513392857136 2201 25 -0.16249718163781068 2201 26 1.04092318272005 2201 27 -1.497038465007222 2201 28 1.5620631538600236 2201 29 2.351369724025986 2201 30 1.374753675144305 2201 31 2.245340683621933 2201 32 -0.3671204229797951 2201 33 2.4961264430014367 2201 34 1.380491296897553 2201 35 -0.16376093524529978 2201 36 -0.8934067234848513 2201 37 2.8098591946248206 2201 38 2.598872091450211 2201 39 -0.21973642676766758 2201 40 2.4779198232323125 2201 41 1.425062567640687 2201 42 -0.4930369543650741 2201 43 2.7762462797618923 2201 44 -0.8724471275252483 2201 45 -1.6751166801947974 2201 46 3.0956185741341997 2201 47 -1.4114662247474783 2201 48 -1.394340165043289 2201 49 -1.5377807088744646 2201 50 -1.649429563492049 2201 51 -7.258628697691197 1101 1 -0.11651278409091503 1101 2 -2.710831529581533 1101 3 1.1552652642496355 1101 4 0.8851579410173116 1101 5 -1.7535832656926402 1101 6 -4.02847053120491 1101 7 -2.607751059704178 1101 8 -6.3672269570707165 1101 9 -0.05973349567099007 1101 10 -4.084911616161598 1101 11 -1.268851461038956 1101 12 -2.486367582070713 1101 13 -4.2478276064213745 1101 14 -2.6460937499999986 1101 15 -1.8148155663780585 1101 16 2.90280427038239 1101 17 1.8369436553030312 1101 18 -7.180093344155843 1101 19 -3.3360722177128466 1101 20 -3.3647885101010004 1101 21 -2.981946699134191 1101 22 0.00202189303752931 1101 23 0.8175211940837102 1101 24 -3.5428199404761997 1101 25 -1.9482114673520954 1101 26 -4.482886341089472 1101 27 0.14581867784992397 1101 28 -2.961746369949495 1101 29 -0.8629159902597294 1101 30 -2.982389181998549 1101 31 2.197721636002882 1101 32 -1.6528347086940798 1101 33 -3.5514926046176107 1101 34 -5.476651560245301 1101 35 0.050524779040415524 1101 36 -2.1553114853896176 1101 37 -1.6901408053751794 1101 38 -0.94874695616884 1101 39 -0.3864030934343319 1101 40 -6.093508748196257 1101 41 -5.955889813311696 1101 42 -4.873989335317457 1101 43 0.2762462797618923 1101 44 -2.9676852227633432 1101 45 -1.4846404897186076 1101 46 -5.47580999729437 1101 47 -0.9590852723665293 1101 48 -4.894340165043289 1101 49 -3.585399756493512 1101 50 0.8029513888888999 1101 51 -4.83005726911977 2202 1 2.1692015016233697 2202 2 0.5510732323232332 2202 3 0.5838366928210661 2202 4 2.0280150838744575 2202 5 -1.2059642180735892 2202 6 1.3048628021284259 2202 7 2.4160584641053404 2202 8 2.537534947691185 2202 9 1.6783617424242436 2202 10 3.0103264790764968 2202 11 2.088291396103898 2202 12 2.704108608405477 2202 13 -2.5573514159451847 2202 14 1.6158110119047677 2202 15 0.5899463383838466 2202 16 3.045661413239536 2202 17 2.122657941017316 2202 18 1.8199066558441572 2202 19 3.2591658775252483 2202 20 3.4447352994228098 2202 21 2.970434253246758 2202 22 1.3115457025613395 2202 23 2.3889497655122796 2202 24 1.7190848214285666 2202 25 -0.16249718163781068 2202 26 0.18378032557719592 2202 27 -0.9256098935786454 2202 28 2.7049202967171695 2202 29 1.9227982954545553 2202 30 3.374753675144305 2202 31 -0.18323074494949765 2202 32 1.0614510055916355 2202 33 2.7818407287157214 2202 34 1.380491296897553 2202 35 2.5505247790404155 2202 36 2.3923075622294334 2202 37 1.6670020517676747 2202 38 1.7417292343073498 2202 39 2.208835001803763 2202 40 1.4779198232323125 2202 41 1.8536339962121176 2202 42 2.7926773313492106 2202 43 -0.7951822916666771 2202 44 -1.158161413239533 2202 45 -1.6751166801947974 2202 46 -4.904381425865797 2202 47 -0.1257519390331936 2202 48 0.748516977813857 2202 49 1.74793357683982 2202 50 -1.5065724206349103 2202 51 1.3127998737373758 1102 1 -1.366512784090915 1102 2 0.8725018037518026 1102 3 -2.2197347357503645 1102 4 -1.7398420589826884 1102 5 0.8297500676406955 1102 6 -5.695137197871574 1102 7 -3.6910843930375137 1102 8 -0.7838936237373844 1102 9 -2.7680668290043258 1102 10 -2.293244949494934 1102 11 -1.018851461038956 1102 12 -3.6530342487373773 1102 13 1.8355057269119612 1102 14 -0.6877604166666629 1102 15 -3.356482233044723 1102 16 -3.47219572961761 1102 17 -5.913056344696969 1102 18 -5.555093344155843 1102 19 -6.294405551046182 1102 20 -5.323121843434336 1102 21 -1.940280032467527 1102 22 -4.581311440295806 1102 23 -1.0574788059162898 1102 24 0.790513392857136 1102 25 -2.4482114673520954 1102 26 0.3087803255771959 1102 27 1.395818677849924 1102 28 -0.9200797032828305 1102 29 -4.862915990259729 1102 30 -1.357389181998549 1102 31 -0.21894503066378235 1102 32 -7.27783470869408 1102 33 -3.4681592712842786 1102 34 -4.476651560245301 1102 35 -2.4494752209595845 1102 36 -0.19697815205628189 1102 37 1.3098591946248206 1102 38 -1.7404136228355043 1102 39 1.7802635732323324 1102 40 -2.843508748196257 1102 41 -3.9142231466450284 1102 42 -2.8323226686507894 1102 43 -5.598753720238108 1102 44 -2.551018556096679 1102 45 -1.0679738230519433 1102 46 -4.47580999729437 1102 47 -1.5007519390331936 1102 48 -3.144340165043289 1102 49 2.49793357683982 1102 50 -4.113715277777764 1102 51 1.29494273088023 2203 1 -0.741512784090915 2203 2 1.6225018037518026 2203 3 1.6552652642496355 2203 4 2.8851579410173116 2203 5 0.20475006764069548 2203 6 -0.32013719787157413 2203 7 0.18391560696248632 2203 8 -0.15889362373738436 2203 9 0.48193317099567423 2203 10 1.4567550505050662 2203 11 -2.643851461038956 2203 12 1.4719657512626227 2203 13 1.4605057269119612 2203 14 2.937239583333337 2203 15 1.6435177669552772 2203 16 -0.72219572961761 2203 17 0.8369436553030312 2203 18 1.1949066558441572 2203 19 1.8305944489538177 2203 20 2.551878156565664 2203 21 0.059719967532473106 2203 22 2.0436885597041936 2203 23 -2.93247880591629 2203 24 -2.209486607142864 2203 25 0.17678853264790462 2203 26 -1.441219674422804 2203 27 -0.854181322150076 2203 28 0.07992029671716949 2203 29 -4.112915990259729 2203 30 -0.6073891819985491 2203 31 1.1560549693362177 2203 32 -3.15283470869408 2203 33 -4.343159271284279 2203 34 -4.351651560245301 2203 35 -1.0744752209595845 2203 36 -1.6969781520562819 2203 37 1.9348591946248206 2203 38 1.2595863771644957 2203 39 -0.09473642676766758 2203 40 1.0314912518037431 2203 41 0.7107768533549716 2203 42 -1.0823226686507894 2203 43 -0.09875372023810769 2203 44 2.073981443903321 2203 45 1.1820261769480567 2203 46 3.2741900027056303 2203 47 3.3742480609668064 2203 48 0.48065983495671105 2203 49 1.12293357683982 2203 50 -3.6137152777777644 2203 51 0.16994273088022993 1103 1 -0.25936992694806094 1103 2 -3.734641053391055 1103 3 -3.701877592893222 1103 4 -2.1148420589826884 1103 5 -1.3488213609307351 1103 6 -1.4094229121572894 1103 7 -1.8696558216089443 1103 8 -6.176750766594527 1103 9 -4.035923971861472 1103 10 -1.275387806637788 1103 11 -3.1974228896103867 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1209 24 2.790513392857136 1209 25 3.3017885326479046 1209 26 0.3087803255771959 1209 27 -0.47918132215007603 1209 28 -0.5450797032828305 1209 29 1.0120840097402706 1209 30 -0.9823891819985491 1209 31 -1.4689450306637823 1209 32 1.3471652913059202 1209 33 -3.7181592712842786 1209 34 -0.1016515602453012 1209 35 -4.6994752209595845 1209 36 -4.321978152056282 1209 37 -2.1901408053751794 1209 38 -4.115413622835504 1209 39 -2.2197364267676676 1209 40 0.15649125180374313 1209 41 -3.2892231466450284 1209 42 2.5426773313492106 1209 43 3.2762462797618923 1209 44 -1.426018556096679 1209 45 2.4320261769480567 1209 46 -4.22580999729437 1209 47 -7.519390331935938E-4 1209 48 -2.144340165043289 1209 49 -0.6270664231601799 1209 50 -0.48871527777776436 1209 51 0.7949427308802299 1214 1 1.883487215909085 1214 2 1.1225018037518026 1214 3 1.1552652642496355 1214 4 0.8851579410173116 1214 5 1.0797500676406955 1214 6 -0.028470531204909832 1214 7 1.892248940295822 1214 8 0.9661063762626156 1214 9 0.7735998376623385 1214 10 1.5817550505050662 1214 11 0.3978152056277082 1214 12 -1.3197009154040416 1214 13 0.25217239357862553 1214 14 1.8539062500000014 1214 15 1.6851844336219415 1214 16 1.5694709370490543 1214 17 2.836943655303031 1214 18 3.4865733225108215 1214 19 2.1639277822871534 1214 20 2.301878156565664 1214 21 3.3513866341991374 1214 22 1.835355226370858 1214 23 0.8175211940837102 1214 24 -0.37615327380952834 1214 25 -1.281544800685431 1214 26 1.5171136589105316 1214 27 1.9791520111832597 1214 28 2.371586963383834 1214 29 2.303750676406935 1214 30 2.184277484668115 1214 31 1.3643883026695534 1214 32 2.680498624639256 1214 33 3.115174062049057 1214 34 1.190015106421363 1214 35 3.21719144570708 1214 36 3.011355181277054 1214 37 3.476525861291485 1214 38 3.55125304383116 1214 39 2.4469302398989967 1214 40 -0.5935087481962569 1214 41 -0.7892231466450284 1214 42 1.4593439980158749 1214 43 1.609579613095228 1214 44 2.698981443903321 1214 45 2.1820261769480567 1214 46 1.357523336038966 1214 47 1.207581394300142 1214 48 1.4389931682900468 1214 49 3.4146002435064844 1214 50 -0.19704861111110006 1214 51 0.6699427308802299 1215 1 2.883487215909085 1215 2 3.1225018037518026 1215 3 2.1552652642496355 1215 4 -1.1148420589826884 1215 5 1.0797500676406955 1215 6 1.6381961354617616 1215 7 1.5589156069624863 1215 8 1.2994397095959513 1215 9 1.1069331709956742 1215 10 0.5817550505050662 1215 11 1.5644818722943796 1215 12 -0.15303424873737725 1215 13 0.9188390602452969 1215 14 -2.979427083333327 1215 15 0.6851844336219415 1215 16 0.23613760371572567 1215 17 2.170276988636367 1215 18 0.4865733225108215 1215 19 0.16392778228715343 1215 20 -1.5314551767676718 1215 21 -2.1486133658008626 1215 22 1.0020218930375293 1215 23 0.31752119408371016 1215 24 0.790513392857136 1215 25 -0.6148781340187597 1215 26 1.8504469922438602 1215 27 1.645818677849924 1215 28 -1.1284130366161662 1215 29 2.303750676406935 1215 30 0.017610818001450923 1215 31 1.0310549693362177 1215 32 0.8471652913059202 1215 33 3.115174062049057 1215 34 -2.309984893578637 1215 35 -2.9494752209595845 1215 36 3.3446885146103824 1215 37 3.8098591946248206 1215 38 2.8845863771644957 1215 39 1.4469302398989967 1215 40 2.239824585137079 1215 41 -3.455889813311696 1215 42 -3.3739893353174573 1215 43 -9.390420386904776 1215 44 0.8656481105699854 1215 45 0.18202617694805667 1215 46 0.5241900027056303 1215 47 -1.9590852723665293 1215 48 -0.061006831709953246 1215 49 -5.25206642316018 1215 50 -4.030381944444432 1215 51 2.8366093975468942 1212 1 -2.3165127840909143 1212 2 -0.4774981962481988 1212 3 -3.044734735750364 1212 4 -3.3148420589826877 1212 5 -0.3202499323593031 1212 6 1.7048628021284244 1212 7 -0.8410843930375123 1212 8 2.766106376262613 1212 9 1.5069331709956728 1212 10 0.781755050505069 1212 11 0.8311485389610453 1212 12 2.64696575126262 1212 13 2.3855057269119584 1212 14 0.7872395833333385 1212 15 2.8185177669552743 1212 16 0.7028042703823871 1212 17 -2.7630563446969703 1212 18 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-1.6148420589826884 1213 5 -4.6702499323593045 1213 6 0.30486280212842587 1213 7 2.0589156069624863 1213 8 2.4661063762626156 1213 9 0.10693317099567423 1213 10 1.0817550505050662 1213 11 -1.268851461038956 1213 12 -0.6530342487373773 1213 13 -0.9144942730880388 1213 14 -1.5627604166666629 1213 15 -1.7314822330447228 1213 16 0.40280427038239 1213 17 -0.6630563446969688 1213 18 0.8199066558441572 1213 19 -0.41940555104618227 1213 20 1.3018781565656639 1213 21 1.184719967532473 1213 22 3.1686885597041936 1213 23 2.81752119408371 1213 24 2.290513392857136 1213 25 3.0517885326479046 1213 26 2.683780325577196 1213 27 1.645818677849924 1213 28 -6.5450797032828305 1213 29 -0.3629159902597294 1213 30 1.017610818001451 1213 31 1.5310549693362177 1213 32 0.8471652913059202 1213 33 1.7818407287157214 1213 34 1.5233484397546988 1213 35 -2.4494752209595845 1213 36 -1.0719781520562819 1213 37 -4.940140805375179 1213 38 -1.3654136228355043 1213 39 -2.9697364267676676 1213 40 1.4064912518037431 1213 41 1.2107768533549716 1213 42 1.7926773313492106 1213 43 -1.4737537202381077 1213 44 -3.551018556096679 1213 45 -4.067973823051943 1213 46 -11.47580999729437 1213 47 -1.3757519390331936 1213 48 -6.394340165043289 1213 49 0.4979335768398201 1213 50 -1.6137152777777644 1213 51 0.9199427308802299 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 1.0 22 2.0 23 16.0 24 17.0 25 50.0 26 57.0 27 102.0 28 100.0 29 131.0 30 167.0 31 195.0 32 231.0 33 278.0 34 297.0 35 358.0 36 433.0 37 561.0 38 861.0 39 846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.4750265674814 16.259298618490966 11.370882040382572 38.89479277364506 2 12.77364505844846 19.596174282678003 44.93092454835282 22.69925611052072 3 14.771519659936239 23.294367693942615 39.192348565356006 22.741764080765144 4 12.114771519659936 27.20510095642933 33.156216790648244 27.523910733262486 5 12.964930924548353 31.200850159404887 42.975557917109455 12.858660998937301 6 25.44102019128587 45.78108395324123 15.876726886291179 12.901168969181724 7 23.69819341126461 28.076514346439957 21.275239107332624 26.950053134962804 8 20.382571732199786 44.50584484590861 18.873538788522847 16.238044633368755 9 21.59404888416578 15.96174282678002 18.46971307120085 43.974495217853345 10 13.41126461211477 38.3634431455898 28.5653560042508 19.659936238044633 11 28.395324123273113 21.764080765143465 32.4548352816153 17.38575982996812 12 19.149840595111584 22.76301806588735 27.863974495217853 30.22316684378321 13 23.719447396386823 20.680127523910734 34.24017003188098 21.360255047821468 14 18.46971307120085 19.319872476089266 25.334750265674817 36.87566418703507 15 20.57385759829968 26.546227417640804 21.317747077577046 31.562167906482465 16 30.58448459086079 25.9086078639745 23.50690754516472 20.0 17 20.892667375132838 36.47183846971307 24.6971307120085 17.93836344314559 18 31.094580233793835 24.803400637619553 25.015940488841657 19.08607863974495 19 21.211477151965994 24.14452709883103 23.08182784272051 31.562167906482465 20 20.297555791710945 24.399574920297557 23.018065887353877 32.28480340063762 21 19.128586609989373 25.58979808714134 23.846971307120086 31.4346439957492 22 19.46865037194474 36.06801275239107 24.05951115834219 20.403825717321997 23 20.063761955366633 24.739638682252924 23.379383634431456 31.817215727948987 24 30.712008501594052 25.54729011689692 24.293304994686505 19.44739638682253 25 21.402763018065887 33.77258235919235 23.52816153028693 21.296493092454835 26 31.0308182784272 25.483528161530288 23.931987247608927 19.55366631243358 27 20.488841657810838 36.47183846971307 23.12433581296493 19.91498405951116 28 19.53241232731137 24.760892667375135 24.6971307120085 31.00956429330499 29 25.16471838469713 25.185972369819343 28.055260361317746 21.59404888416578 30 22.592986184909673 29.373007438894795 27.906482465462272 20.127523910733263 31 21.190223166843783 27.842720510095642 29.11795961742827 21.849096705632306 32 21.04144527098831 25.759829968119025 29.394261424017003 23.804463336875664 33 23.188097768331563 25.674814027630184 29.415515409139214 21.721572794899043 34 19.95749202975558 26.907545164718382 32.41232731137088 20.722635494155153 35 25.015940488841657 25.228480340063765 28.629117959617428 21.126461211477153 36 21.679064824654624 26.695005313496278 30.988310308182783 20.637619553666312 37 18.660998937300743 34.261424017003186 26.35494155154091 20.722635494155153 38 18.06588735387885 21.105207226354942 38.34218916046759 22.48671625929862 39 17.662061636556857 20.106269925611052 29.11795961742827 33.11370882040382 40 17.279489904357067 21.08395324123273 39.46865037194474 22.16790648246546 41 18.02337938363443 20.276301806588734 29.075451647183847 32.624867162592984 42 28.862911795961743 20.935175345377257 28.71413390010627 21.487778958554728 43 16.068012752391073 21.08395324123273 40.191285866099896 22.6567481402763 44 14.962805526036133 32.94367693942614 29.67056323060574 22.422954303931988 45 14.133900106269925 22.231668437832095 40.99893730074389 22.635494155154092 46 25.54729011689692 21.91285866099894 29.543039319872477 22.996811902231666 47 14.048884165781084 20.99893730074389 30.797024442082893 34.155154091392134 48 14.622741764080766 22.06163655685441 28.24654622741764 35.06907545164719 49 23.846971307120086 21.934112646121147 29.11795961742827 25.100956429330502 50 14.070138150903293 22.48671625929862 40.255047821466526 23.188097768331563 51 13.36875664187035 22.6567481402763 27.672688629117957 36.30180658873539 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 2.0 3 1.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 1.0 11 1.0 12 1.0 13 2.5 14 4.0 15 3.0 16 2.0 17 3.0 18 4.0 19 2.5 20 1.0 21 2.0 22 3.0 23 4.0 24 5.0 25 5.0 26 13.0 27 21.0 28 36.5 29 52.0 30 66.5 31 81.0 32 120.0 33 159.0 34 201.0 35 243.0 36 265.0 37 287.0 38 308.0 39 329.0 40 345.0 41 361.0 42 383.0 43 405.0 44 415.0 45 425.0 46 407.5 47 390.0 48 465.0 49 540.0 50 508.0 51 476.0 52 424.5 53 373.0 54 275.0 55 177.0 56 147.5 57 118.0 58 96.5 59 75.0 60 68.0 61 61.0 62 48.0 63 35.0 64 31.0 65 27.0 66 22.5 67 18.0 68 11.0 69 4.0 70 7.5 71 11.0 72 7.0 73 3.0 74 3.0 75 2.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4705.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.90541976620617 #Duplication Level Percentage of deduplicated Percentage of total 1 99.09156108056419 88.09776833156216 2 0.3107817355964619 0.5526036131774709 3 0.14343772412144393 0.38257173219978746 4 0.09562514941429597 0.34006376195536664 5 0.07171886206072196 0.3188097768331562 6 0.0 0.0 7 0.0 0.0 8 0.02390628735357399 0.17003188097768332 9 0.0 0.0 >10 0.16734401147501793 3.4218916046758765 >50 0.07171886206072196 4.420828905419767 >100 0.02390628735357399 2.2954303931987248 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 108 2.2954303931987248 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 78 1.6578108395324123 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC 69 1.4665249734325185 No Hit CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 61 1.2964930924548352 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 49 1.0414452709883104 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 39 0.8289054197662061 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 20 0.4250797024442083 No Hit CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC 16 0.34006376195536664 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 14 0.2975557917109458 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 12 0.255047821466525 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT 11 0.23379383634431458 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 8 0.17003188097768332 No Hit CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC 5 0.10626992561105207 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 5 0.10626992561105207 No Hit CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC 5 0.10626992561105207 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.021253985122210415 2 0.0 0.0 0.021253985122210415 3 0.0 0.0 0.021253985122210415 4 0.0 0.0 0.021253985122210415 5 0.0 0.0 0.021253985122210415 6 0.0 0.0 0.021253985122210415 7 0.0 0.0 0.021253985122210415 8 0.0 0.0 0.021253985122210415 9 0.0 0.0 0.021253985122210415 10 0.0 0.0 0.021253985122210415 11 0.0 0.0 0.021253985122210415 12 0.0 0.0 0.021253985122210415 13 0.0 0.0 0.021253985122210415 14 0.0 0.0 0.021253985122210415 15 0.0 0.0 0.021253985122210415 16 0.0 0.0 0.021253985122210415 17 0.0 0.0 0.021253985122210415 18 0.0 0.0 0.04250797024442083 19 0.0 0.0 0.04250797024442083 20 0.0 0.0 0.04250797024442083 21 0.0 0.0 0.04250797024442083 22 0.0 0.0 0.06376195536663125 23 0.0 0.0 0.06376195536663125 24 0.0 0.0 0.10626992561105207 25 0.0 0.0 0.1275239107332625 26 0.0 0.0 0.17003188097768332 27 0.0 0.0 0.23379383634431455 28 0.0 0.0 0.5951115834218916 29 0.0 0.0 0.9776833156216791 30 0.0 0.0 1.6153028692879916 31 0.0 0.0 2.7417640807651433 32 0.0 0.0 3.8682252922422955 33 0.0 0.0 5.079702444208289 34 0.0 0.0 6.503719447396387 35 0.0 0.0 7.991498405951116 36 0.0 0.0 9.500531349628055 37 0.0 0.0 11.689691817215728 38 0.0 0.0 13.751328374070138 39 0.0 0.0 16.195536663124336 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGATC 25 3.3283784E-5 45.0 33 GTATGCC 80 0.0 45.0 42 ACTAAGC 25 3.3283784E-5 45.0 27 TGCCGTC 80 0.0 45.0 45 GTGTATC 25 3.3283784E-5 45.0 33 ATGATCT 25 3.3283784E-5 45.0 34 TAAGCAT 25 3.3283784E-5 45.0 29 TGATCTC 25 3.3283784E-5 45.0 35 TATGCCG 80 0.0 45.0 43 GTATCTC 40 4.667527E-9 45.0 35 GATCGTG 25 3.3283784E-5 45.0 29 TCGTGTA 25 3.3283784E-5 45.0 31 TATCTCG 50 1.2732926E-11 45.0 36 AGACGAT 25 3.3283784E-5 45.0 25 GATCTCG 25 3.3283784E-5 45.0 36 ATGCCGT 80 0.0 45.0 44 CGTATGC 80 0.0 45.0 41 GACGATC 25 3.3283784E-5 45.0 26 GCATGAT 25 3.3283784E-5 45.0 32 CGTGTAT 25 3.3283784E-5 45.0 32 >>END_MODULE