FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_61.341000000065ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_61.341000000065ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4705
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC1082.2954303931987248No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC781.6578108395324123No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC691.4665249734325185No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC611.2964930924548352No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC491.0414452709883104RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC390.8289054197662061RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC200.4250797024442083No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC160.34006376195536664No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC140.2975557917109458RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC120.255047821466525No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT110.23379383634431458No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC80.17003188097768332No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC50.10626992561105207No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50.10626992561105207No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC50.10626992561105207No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGATC253.3283784E-545.033
GTATGCC800.045.042
ACTAAGC253.3283784E-545.027
TGCCGTC800.045.045
GTGTATC253.3283784E-545.033
ATGATCT253.3283784E-545.034
TAAGCAT253.3283784E-545.029
TGATCTC253.3283784E-545.035
TATGCCG800.045.043
GTATCTC404.667527E-945.035
GATCGTG253.3283784E-545.029
TCGTGTA253.3283784E-545.031
TATCTCG501.2732926E-1145.036
AGACGAT253.3283784E-545.025
GATCTCG253.3283784E-545.036
ATGCCGT800.045.044
CGTATGC800.045.041
GACGATC253.3283784E-545.026
GCATGAT253.3283784E-545.032
CGTGTAT253.3283784E-545.032