FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_60.341000000065df.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_60.341000000065df.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC701.9536701088473347No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC401.1163829193413342No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC361.0047446274072007No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC350.9768350544236674RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC200.5581914596706671No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC200.5581914596706671No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC120.3349148758024002No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC110.30700530281886684No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC90.2511861568518002No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC80.22327658386826682No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC70.19536701088473346RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC60.1674574379012001No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT60.1674574379012001No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC60.1674574379012001No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGT50.13954786491766677No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC50.13954786491766677No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG50.13954786491766677No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC40.11163829193413341RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC40.11163829193413341No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC40.11163829193413341TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCGACGGCATCTCGTATGCCGTC40.11163829193413341No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT40.11163829193413341No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC40.11163829193413341No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)