FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_59.341000000065c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_59.341000000065c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422312
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG401.558464E-533.7492940
TACTGTA300.00514116730.0029182
TATGCCG453.486171E-529.99936943
GGCGAAT406.160394E-428.12440732
GCGCAAT500.002262532722.49952721
GGTATCA6950.021.705611
ACGGGTC654.232718E-420.76879316
GTGTAGG1251.7455022E-819.813661
GTATCAA12200.019.7472211
ACGGGAC1850.019.4590516
CTCGTAT706.9744507E-419.28530739
ACGAGAC951.3128527E-518.9469722
TCTCCGA3350.018.80780245
CAAGGCG600.00650462718.74960529
ACAGCGC600.00650462718.7496058
CGCAATC600.00650462718.74960522
ATCTCCG5050.018.71469345
CACGAGA750.001108817.9996221
TCTCGTA750.001108817.9996238
AATCTCG750.001108817.9996236
Produced by FastQC (version 0.11.2)