FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_58.341000000065b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_58.341000000065b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398970
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC6570.16467403564177757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.029.4709361
GTATCAA8750.025.2056871
ATAATAA450.001225329824.99937429
TATGCCG851.8876563E-723.82293143
CGGGCTC955.5077544E-721.31792817
ATCTCCG3550.020.91496845
GCCCACG1109.9325916E-820.45659618
TTAATCC550.00393267520.4540314
CCCACGA1109.94587E-820.45403119
GGGCCGC550.00393267520.4540316
CGGGGGG1009.015839E-720.25203117
TATCAAC10900.020.0224342
CGGGGAG807.5413875E-519.68947417
ATCAACG11400.019.341623
GGCGAAT1051.4410853E-619.2852332
TCAACGC11550.019.285234
TACGGGC2750.018.82006815
CTCGTAT1202.4871588E-718.74952939
CAACGCA11800.018.6859725
AACGCAG11850.018.607136
Produced by FastQC (version 0.11.2)