FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_57.341000000065a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_57.341000000065a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252061
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGCG356.2292766E-638.57108729
AAGCCGT250.002104086835.999686
TATGCCG458.664738E-734.9996943
ACGAGAC458.664738E-734.9996922
AGGCGAA401.5558502E-533.74970231
GGCGAAT401.5558502E-533.74970232
GATATAG453.4709436E-530.0116421
CGAGACC453.4803634E-529.99973523
TATAGGC507.1370516E-526.9997623
GCCCACG608.158993E-626.24976718
CCCACGA608.158993E-626.24976719
TCGTATG608.158993E-626.24976740
ATATAGG450.001223961924.999782
GCGAATC551.36415E-424.54523833
AATCTCG551.36415E-424.54523836
TGCCGTC651.5177311E-524.23055645
AGCCCAC751.6890244E-623.99978617
CCACGAG751.6890244E-623.99978620
CGAATCT602.4602495E-422.499834
TAAATTA500.00225986122.499823
Produced by FastQC (version 0.11.2)