FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_55.3410000000658f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_55.3410000000658f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539884
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTAC300.005141814730.0036431
TCGTATA300.005144144430.0008642
TATGCCG852.0372681E-1029.11579143
GTATCAA9300.025.8902441
CGTATGC1001.3424142E-924.7484241
TCGTATG1052.3610482E-923.56992540
CGAGACG500.00226025222.50481823
CCACTAG500.002261469122.5027331
ACCGAGC500.002263905222.4985668
CGAACGT702.7008948E-522.4985644
TATCTCG702.7008948E-522.49856436
GGTATCA4250.021.1790431
CGTTTAG654.2333963E-420.7698313
TCGAACG654.236044E-420.7679063
TACGGGA4450.020.73093415
AACGCAG11700.020.5756116
AACCGTG550.003935328720.4532417
ATCTCCG8750.020.3129945
CAACGCA11900.020.0407375
CTCGTAT1251.7633283E-819.79873739
Produced by FastQC (version 0.11.2)