FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_54.34100000006572.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_54.34100000006572.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536656
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA300.00514067730.0049691
TATGCCG602.415345E-729.99937843
CCCACGA751.6951417E-623.99950419
GTATCAA12600.023.2181321
CGGGGCC500.002262095722.5016317
TGCCGTC802.9679868E-622.50162945
TCGTATG855.0248636E-621.17603140
TCTAAAC550.003934317720.454123
CACGAGA908.239269E-619.99958621
GGTATCA6550.019.9269661
TACCGGC1251.7624188E-819.7995917
TGTAGGG1503.43789E-1019.4995982
CGAGACG706.977804E-419.28531523
CATCTCC23550.019.01411645
CTCCGAG4650.018.87233545
GTTTACG600.006499860418.7531051
TTTGCGT1454.7566573E-918.6203043
ACACTTT851.2031144E-418.5290268
TTGCGTT1608.1308826E-1018.280874
AACGCAG16050.018.0837386
Produced by FastQC (version 0.11.2)