FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_53.34100000006565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_53.34100000006565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610681
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC352.813546E-432.1531561
TATCTCG708.1308826E-1032.14262436
CTCGTAT1250.026.99980539
TATGCCG1250.026.99980543
TCGTATG1300.025.9613540
CGAGACG1101.5097612E-1024.54527723
CGTATGC1450.023.27569441
GACGATA500.002263499722.49983626
TCTCGTA1551.8189894E-1221.77403538
GGGACGC754.5983543E-520.99984719
CGGGACG754.5983543E-520.99984718
ACGAGAC1750.020.5712822
CACGAGA1800.019.99985321
AATACGA807.556009E-519.6873572
ATCTCGT1850.019.45931837
CAATACG706.9633115E-419.2918931
ATGCCGT1757.2759576E-1219.28557644
TAAACTC600.006507356718.7498635
GCCGGCA600.006507356718.74986313
ACGATAA600.006507356718.74986327
Produced by FastQC (version 0.11.2)