FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_52.34100000006559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_52.34100000006559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293222
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCACGC401.5570646E-533.7485928
TATGCCG602.4094697E-729.9987543
CGTATGC654.854319E-727.69115441
GTAGGGC551.3651967E-424.5444323
CTCGTTT751.6910781E-623.9989998
CTCGTAT751.6910781E-623.99899939
ACGGGTT602.4621267E-422.49906316
TCGTTTA802.9633065E-622.4990629
CGGGGTG2100.022.49906217
CGTTTAG802.9633065E-622.49906210
GTATGCC855.0129456E-621.17558942
TCGTATG855.0129456E-621.17558940
ACGAGAC855.0129456E-621.17558922
TGCCGTC855.0129456E-621.17558945
TTAGCCG754.5891073E-520.99912513
GGAACAC654.2290526E-420.7683665
CTAATTA654.2290526E-420.76836633
GGACTAA1201.0972144E-820.62414219
TAGATCA550.003930603620.4536934
TGTAGGG550.003930603620.4536932
Produced by FastQC (version 0.11.2)