FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_51.3410000000654c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_51.3410000000654c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528187
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT453.4861077E-530.00151416
AACCTAT300.00514588129.99867432
GCTCGCA406.1598775E-428.1264215
ATCGTTA450.001225992824.99889629
TATGCCG651.5222464E-524.22969843
GTCCTAT500.002256120822.5117931
ATCGGAA500.002262337822.50113713
TCGTATG702.7003423E-522.49900640
ACGTATT602.4657973E-422.49900617
TCTCGTA754.5981527E-520.99907138
CTACGTA654.2326096E-420.77027915
CGCAATG654.2353154E-420.76831218
ATCTCGC550.003934771320.4536426
TAATTCA807.5556876E-519.686635
ATCTCGT1151.5919977E-719.56435437
GCCCACG1151.5919977E-719.56435418
ACGAGAC1051.4418492E-619.28668822
CCACGAG951.3130613E-518.94832420
TATCTCG951.314134E-518.94653136
TGCTCGC600.00650404918.75094614
Produced by FastQC (version 0.11.2)