FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 24h_50.3410000000653f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 24h_50.3410000000653f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373703
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGATT250.002104524836.00267826
TGCCCGC250.002105907535.9978612
CTAGTCA300.005144361429.9982153
TAGTCAT300.005144361429.9982154
CGATCAA406.1556185E-428.1270910
GGTATCA4750.023.6986581
GCGTCAG500.002253770422.5137231
GTATCAA8900.021.5018711
CCTATCA754.5893357E-521.0015628
ACGGGTA754.5940717E-520.9987516
TAGGACA754.5940717E-520.998754
CCCTATC654.2287365E-420.7707757
TGGGCCG550.003929950820.4560665
GACTAAT1109.945143E-820.4533320
TCTCCGA5450.020.22815545
GCCCTAT706.9679174E-419.2871486
TACTGGA600.00649967618.7513942
AGTCGCC600.00649967618.75139427
TAACATC600.006504718.7488844
CGTTTTG600.006504718.74888433